IFN-β-inducing, unusual viral RNA species produced by paramyxovirus infection accumulated into distinct cytoplasmic structures in an RNA-type-dependent manner
The interferon (IFN) system is one of the most important defensive responses of mammals against viruses, and is rapidly evoked when the pathogen-associated molecular patterns (PAMPs) of viruses are sensed. Non-self, virus-derived RNA species have been identified as the PAMPs of RNA viruses. In the p...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2015-08-01
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| Series: | Frontiers in Microbiology |
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| Online Access: | http://journal.frontiersin.org/Journal/10.3389/fmicb.2015.00804/full |
| _version_ | 1831669807472181248 |
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| author | Asuka eYoshida Ryoko eKawabata Tomoyuki eHonda Keizo eTomonaga Takemasa eSakaguchi Takashi eIrie |
| author_facet | Asuka eYoshida Ryoko eKawabata Tomoyuki eHonda Keizo eTomonaga Takemasa eSakaguchi Takashi eIrie |
| author_sort | Asuka eYoshida |
| collection | DOAJ |
| description | The interferon (IFN) system is one of the most important defensive responses of mammals against viruses, and is rapidly evoked when the pathogen-associated molecular patterns (PAMPs) of viruses are sensed. Non-self, virus-derived RNA species have been identified as the PAMPs of RNA viruses. In the present study, we compared different types of IFN-β-inducing and -non-inducing viruses in the context of Sendai virus (SeV) infection. We found that some types of unusual viral RNA species were produced by infections with IFN-β-inducing viruses and accumulated into distinct cytoplasmic structures in an RNA-type-dependent manner. One of these structures was similar to the so-called antiviral stress granules (avSGs) formed by an infection with IFN-inducing viruses whose C proteins were knocked-out or mutated. Non-encapsidated, unusual viral RNA harboring the 5’-terminal region of the viral genome as well as RIG-I and typical SG markers accumulated in these granules. Another was a non-SG-like inclusion formed by an infection with the Cantell strain; a copyback-type DI genome, but not an authentic viral genome, specifically accumulated in the inclusion, whereas RIG-I and SG markers did not. The induction of IFN-β was closely associated with the production of these unusual RNAs as well as the formation of the cytoplasmic structures. |
| first_indexed | 2024-12-19T23:06:14Z |
| format | Article |
| id | doaj.art-a8df9fdb5a574482b2b0c3d15cc65d8c |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-12-19T23:06:14Z |
| publishDate | 2015-08-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj.art-a8df9fdb5a574482b2b0c3d15cc65d8c2022-12-21T20:02:22ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2015-08-01610.3389/fmicb.2015.00804155383IFN-β-inducing, unusual viral RNA species produced by paramyxovirus infection accumulated into distinct cytoplasmic structures in an RNA-type-dependent mannerAsuka eYoshida0Ryoko eKawabata1Tomoyuki eHonda2Keizo eTomonaga3Takemasa eSakaguchi4Takashi eIrie5Institute of Biomedical & Health Sciences, Hiroshima UniversityInstitute of Biomedical & Health Sciences, Hiroshima UniversityInstitute for Virus Research, Kyoto UniversityInstitute for Virus Research, Kyoto UniversityInstitute of Biomedical & Health Sciences, Hiroshima UniversityInstitute of Biomedical & Health Sciences, Hiroshima UniversityThe interferon (IFN) system is one of the most important defensive responses of mammals against viruses, and is rapidly evoked when the pathogen-associated molecular patterns (PAMPs) of viruses are sensed. Non-self, virus-derived RNA species have been identified as the PAMPs of RNA viruses. In the present study, we compared different types of IFN-β-inducing and -non-inducing viruses in the context of Sendai virus (SeV) infection. We found that some types of unusual viral RNA species were produced by infections with IFN-β-inducing viruses and accumulated into distinct cytoplasmic structures in an RNA-type-dependent manner. One of these structures was similar to the so-called antiviral stress granules (avSGs) formed by an infection with IFN-inducing viruses whose C proteins were knocked-out or mutated. Non-encapsidated, unusual viral RNA harboring the 5’-terminal region of the viral genome as well as RIG-I and typical SG markers accumulated in these granules. Another was a non-SG-like inclusion formed by an infection with the Cantell strain; a copyback-type DI genome, but not an authentic viral genome, specifically accumulated in the inclusion, whereas RIG-I and SG markers did not. The induction of IFN-β was closely associated with the production of these unusual RNAs as well as the formation of the cytoplasmic structures.http://journal.frontiersin.org/Journal/10.3389/fmicb.2015.00804/fullRNA VirusesSendai virus (SeV)innate immunity and responsesPAMPs (Pathogen-associated molecular patterns)stress granules |
| spellingShingle | Asuka eYoshida Ryoko eKawabata Tomoyuki eHonda Keizo eTomonaga Takemasa eSakaguchi Takashi eIrie IFN-β-inducing, unusual viral RNA species produced by paramyxovirus infection accumulated into distinct cytoplasmic structures in an RNA-type-dependent manner Frontiers in Microbiology RNA Viruses Sendai virus (SeV) innate immunity and responses PAMPs (Pathogen-associated molecular patterns) stress granules |
| title | IFN-β-inducing, unusual viral RNA species produced by paramyxovirus infection accumulated into distinct cytoplasmic structures in an RNA-type-dependent manner |
| title_full | IFN-β-inducing, unusual viral RNA species produced by paramyxovirus infection accumulated into distinct cytoplasmic structures in an RNA-type-dependent manner |
| title_fullStr | IFN-β-inducing, unusual viral RNA species produced by paramyxovirus infection accumulated into distinct cytoplasmic structures in an RNA-type-dependent manner |
| title_full_unstemmed | IFN-β-inducing, unusual viral RNA species produced by paramyxovirus infection accumulated into distinct cytoplasmic structures in an RNA-type-dependent manner |
| title_short | IFN-β-inducing, unusual viral RNA species produced by paramyxovirus infection accumulated into distinct cytoplasmic structures in an RNA-type-dependent manner |
| title_sort | ifn β inducing unusual viral rna species produced by paramyxovirus infection accumulated into distinct cytoplasmic structures in an rna type dependent manner |
| topic | RNA Viruses Sendai virus (SeV) innate immunity and responses PAMPs (Pathogen-associated molecular patterns) stress granules |
| url | http://journal.frontiersin.org/Journal/10.3389/fmicb.2015.00804/full |
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