Structural Analysis and Anti-Complement Activity of Polysaccharides from Kjellmaniella crsaaifolia
Two polysaccharides, named KCA and KCW, were extracted from Kjellmaniella crassifolia using dilute hydrochloric acid and water, respectively. Composition analysis showed that these polysaccharides predominantly consisted of fucose, with galactose, mannose and glucuronic acid as minor components. Aft...
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2015-03-01
|
| Series: | Marine Drugs |
| Subjects: | |
| Online Access: | http://www.mdpi.com/1660-3397/13/3/1360 |
| _version_ | 1811278840737038336 |
|---|---|
| author | Wenjing Zhang Weihua Jin Delin Sun Luyu Zhao Jing Wang Delin Duan Quanbin Zhang |
| author_facet | Wenjing Zhang Weihua Jin Delin Sun Luyu Zhao Jing Wang Delin Duan Quanbin Zhang |
| author_sort | Wenjing Zhang |
| collection | DOAJ |
| description | Two polysaccharides, named KCA and KCW, were extracted from Kjellmaniella crassifolia using dilute hydrochloric acid and water, respectively. Composition analysis showed that these polysaccharides predominantly consisted of fucose, with galactose, mannose and glucuronic acid as minor components. After degradation and partial desulfation, electrospray ionization mass spectrometry (ESI-MS) was performed, which showed that the polysaccharides consisted of sulfated fucooligosaccharides, sulfated galactofucooligosaccharides and methyl glycosides of mono-sulfated/multi-sulfated fucooligosaccharides. The structures of the oligomeric fragments were further characterized by electrospray ionization collision-induced dissociation tandem mass spectrometry (ESI-CID-MS2 and ESI-CID-MS3). Moreover, the activity of KCA and KCW against the hemolytic activity of both the classical and alternative complement pathways was determined. The activity of KCA was found to be similar to KCW, suggesting that the method of extraction did not influence the activity. In addition, the degraded polysaccharides (DKCA and DKCW) displayed lower activity levels than the crude polysaccharides (KCA and KCW), indicating that molecular weight had an effect on activity. Moreover, the desulfated fractions (ds-DKCA and ds-DKCW) showed less or no activity, which confirmed that sulfate was important for activity. In conclusion, polysaccharides from K. crassifolia may be good candidates for the treatment of diseases involving the complement pathway. |
| first_indexed | 2024-04-13T00:43:34Z |
| format | Article |
| id | doaj.art-a8e1b8ee41ed438dbafa9788b3bf1158 |
| institution | Directory Open Access Journal |
| issn | 1660-3397 |
| language | English |
| last_indexed | 2024-04-13T00:43:34Z |
| publishDate | 2015-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Marine Drugs |
| spelling | doaj.art-a8e1b8ee41ed438dbafa9788b3bf11582022-12-22T03:10:05ZengMDPI AGMarine Drugs1660-33972015-03-011331360137410.3390/md13031360md13031360Structural Analysis and Anti-Complement Activity of Polysaccharides from Kjellmaniella crsaaifoliaWenjing Zhang0Weihua Jin1Delin Sun2Luyu Zhao3Jing Wang4Delin Duan5Quanbin Zhang6Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, ChinaInstitute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, ChinaHeze Juxinyuan Food Co. Ltd., Heze 274400, ChinaHeze Juxinyuan Food Co. Ltd., Heze 274400, ChinaInstitute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, ChinaInstitute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, ChinaInstitute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, ChinaTwo polysaccharides, named KCA and KCW, were extracted from Kjellmaniella crassifolia using dilute hydrochloric acid and water, respectively. Composition analysis showed that these polysaccharides predominantly consisted of fucose, with galactose, mannose and glucuronic acid as minor components. After degradation and partial desulfation, electrospray ionization mass spectrometry (ESI-MS) was performed, which showed that the polysaccharides consisted of sulfated fucooligosaccharides, sulfated galactofucooligosaccharides and methyl glycosides of mono-sulfated/multi-sulfated fucooligosaccharides. The structures of the oligomeric fragments were further characterized by electrospray ionization collision-induced dissociation tandem mass spectrometry (ESI-CID-MS2 and ESI-CID-MS3). Moreover, the activity of KCA and KCW against the hemolytic activity of both the classical and alternative complement pathways was determined. The activity of KCA was found to be similar to KCW, suggesting that the method of extraction did not influence the activity. In addition, the degraded polysaccharides (DKCA and DKCW) displayed lower activity levels than the crude polysaccharides (KCA and KCW), indicating that molecular weight had an effect on activity. Moreover, the desulfated fractions (ds-DKCA and ds-DKCW) showed less or no activity, which confirmed that sulfate was important for activity. In conclusion, polysaccharides from K. crassifolia may be good candidates for the treatment of diseases involving the complement pathway.http://www.mdpi.com/1660-3397/13/3/1360polysaccharideKjellmaniella crassifoliaanti-complement |
| spellingShingle | Wenjing Zhang Weihua Jin Delin Sun Luyu Zhao Jing Wang Delin Duan Quanbin Zhang Structural Analysis and Anti-Complement Activity of Polysaccharides from Kjellmaniella crsaaifolia Marine Drugs polysaccharide Kjellmaniella crassifolia anti-complement |
| title | Structural Analysis and Anti-Complement Activity of Polysaccharides from Kjellmaniella crsaaifolia |
| title_full | Structural Analysis and Anti-Complement Activity of Polysaccharides from Kjellmaniella crsaaifolia |
| title_fullStr | Structural Analysis and Anti-Complement Activity of Polysaccharides from Kjellmaniella crsaaifolia |
| title_full_unstemmed | Structural Analysis and Anti-Complement Activity of Polysaccharides from Kjellmaniella crsaaifolia |
| title_short | Structural Analysis and Anti-Complement Activity of Polysaccharides from Kjellmaniella crsaaifolia |
| title_sort | structural analysis and anti complement activity of polysaccharides from kjellmaniella crsaaifolia |
| topic | polysaccharide Kjellmaniella crassifolia anti-complement |
| url | http://www.mdpi.com/1660-3397/13/3/1360 |
| work_keys_str_mv | AT wenjingzhang structuralanalysisandanticomplementactivityofpolysaccharidesfromkjellmaniellacrsaaifolia AT weihuajin structuralanalysisandanticomplementactivityofpolysaccharidesfromkjellmaniellacrsaaifolia AT delinsun structuralanalysisandanticomplementactivityofpolysaccharidesfromkjellmaniellacrsaaifolia AT luyuzhao structuralanalysisandanticomplementactivityofpolysaccharidesfromkjellmaniellacrsaaifolia AT jingwang structuralanalysisandanticomplementactivityofpolysaccharidesfromkjellmaniellacrsaaifolia AT delinduan structuralanalysisandanticomplementactivityofpolysaccharidesfromkjellmaniellacrsaaifolia AT quanbinzhang structuralanalysisandanticomplementactivityofpolysaccharidesfromkjellmaniellacrsaaifolia |