The effect of ketamine on the immature granulocyte and C-reactive protein concentration in rat models of sepsis
Background: Sepsis directly affects the immune system, leads tissue damage, and forms system organ dysfunction. The immunological biomarker of sepsis has a possibility to become an immunotherapy target of sepsis. This study was conducted to determine the effect of ketamine on the number of immature...
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| Format: | Article |
| Language: | English |
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Wolters Kluwer Medknow Publications
2019-01-01
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| Series: | Bali Journal of Anesthesiology |
| Subjects: | |
| Online Access: | http://www.bjoaonline.com/article.asp?issn=2549-2276;year=2019;volume=3;issue=3;spage=174;epage=177;aulast=Asmoro |
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| author | Aswoco Andyk Asmoro Isngadi Isngadi Ristiawan Muji Laksono Ibnu Firdiansayah Agus Supriyanto |
| author_facet | Aswoco Andyk Asmoro Isngadi Isngadi Ristiawan Muji Laksono Ibnu Firdiansayah Agus Supriyanto |
| author_sort | Aswoco Andyk Asmoro |
| collection | DOAJ |
| description | Background: Sepsis directly affects the immune system, leads tissue damage, and forms system organ dysfunction. The immunological biomarker of sepsis has a possibility to become an immunotherapy target of sepsis. This study was conducted to determine the effect of ketamine on the number of immature granulocyte and C-reactive protein (CRP) concentration in rat models of sepsis. Materials and Methods: This study used thirty white mice (Rattus norvegicus) divided into six treatment groups. The negative control group received nothing, and the positive control (sepsis) group was fecal-induced peritonitis (FIP) by fecal administration (i. p). The treatment groups (A, B, C, and D) treat with ketamine 5 mg/kg body weight (i. p.) right after FIP, 3 h after FIP, 5 h after FIP, and intermittent every 2 h. The peripheral mononuclear blood cell was isolated 6 h after FIP. The immature granulocytes counted using a hematology analyzer while CRP concentration analyzed using kit enzyme-linked immunosorbent assay. Data were analyzed statistically using the one-way ANOVA test using SPSS version 20 software (P < 0.05). Results: Sepsis induction with FIP increases the number of immature granulocytes in animal models from 0.48% to 9.12% (P < 0.05) but did not affect CRP concentration (P > 0.05). The ketamine administration significantly decreases the immature granulocytes in Groups C (1.04%) and D (1.58%). Ketamine administration did not have a significant effect on CRP concentration. Conclusion: The ketamine administration at 5 h after FIP and intermittently every 2 h can be an alternative to be sepsis immunotherapy with immature granulocyte as the potential target. |
| first_indexed | 2024-12-22T15:45:31Z |
| format | Article |
| id | doaj.art-a8e271a4770d4d33b70b9e7bde42b221 |
| institution | Directory Open Access Journal |
| issn | 2549-2276 |
| language | English |
| last_indexed | 2024-12-22T15:45:31Z |
| publishDate | 2019-01-01 |
| publisher | Wolters Kluwer Medknow Publications |
| record_format | Article |
| series | Bali Journal of Anesthesiology |
| spelling | doaj.art-a8e271a4770d4d33b70b9e7bde42b2212022-12-21T18:21:02ZengWolters Kluwer Medknow PublicationsBali Journal of Anesthesiology2549-22762019-01-013317417710.4103/BJOA.BJOA_5_19The effect of ketamine on the immature granulocyte and C-reactive protein concentration in rat models of sepsisAswoco Andyk AsmoroIsngadi IsngadiRistiawan Muji LaksonoIbnu FirdiansayahAgus SupriyantoBackground: Sepsis directly affects the immune system, leads tissue damage, and forms system organ dysfunction. The immunological biomarker of sepsis has a possibility to become an immunotherapy target of sepsis. This study was conducted to determine the effect of ketamine on the number of immature granulocyte and C-reactive protein (CRP) concentration in rat models of sepsis. Materials and Methods: This study used thirty white mice (Rattus norvegicus) divided into six treatment groups. The negative control group received nothing, and the positive control (sepsis) group was fecal-induced peritonitis (FIP) by fecal administration (i. p). The treatment groups (A, B, C, and D) treat with ketamine 5 mg/kg body weight (i. p.) right after FIP, 3 h after FIP, 5 h after FIP, and intermittent every 2 h. The peripheral mononuclear blood cell was isolated 6 h after FIP. The immature granulocytes counted using a hematology analyzer while CRP concentration analyzed using kit enzyme-linked immunosorbent assay. Data were analyzed statistically using the one-way ANOVA test using SPSS version 20 software (P < 0.05). Results: Sepsis induction with FIP increases the number of immature granulocytes in animal models from 0.48% to 9.12% (P < 0.05) but did not affect CRP concentration (P > 0.05). The ketamine administration significantly decreases the immature granulocytes in Groups C (1.04%) and D (1.58%). Ketamine administration did not have a significant effect on CRP concentration. Conclusion: The ketamine administration at 5 h after FIP and intermittently every 2 h can be an alternative to be sepsis immunotherapy with immature granulocyte as the potential target.http://www.bjoaonline.com/article.asp?issn=2549-2276;year=2019;volume=3;issue=3;spage=174;epage=177;aulast=Asmoroc-reactive proteinimmature granulocyteketaminesepsis |
| spellingShingle | Aswoco Andyk Asmoro Isngadi Isngadi Ristiawan Muji Laksono Ibnu Firdiansayah Agus Supriyanto The effect of ketamine on the immature granulocyte and C-reactive protein concentration in rat models of sepsis Bali Journal of Anesthesiology c-reactive protein immature granulocyte ketamine sepsis |
| title | The effect of ketamine on the immature granulocyte and C-reactive protein concentration in rat models of sepsis |
| title_full | The effect of ketamine on the immature granulocyte and C-reactive protein concentration in rat models of sepsis |
| title_fullStr | The effect of ketamine on the immature granulocyte and C-reactive protein concentration in rat models of sepsis |
| title_full_unstemmed | The effect of ketamine on the immature granulocyte and C-reactive protein concentration in rat models of sepsis |
| title_short | The effect of ketamine on the immature granulocyte and C-reactive protein concentration in rat models of sepsis |
| title_sort | effect of ketamine on the immature granulocyte and c reactive protein concentration in rat models of sepsis |
| topic | c-reactive protein immature granulocyte ketamine sepsis |
| url | http://www.bjoaonline.com/article.asp?issn=2549-2276;year=2019;volume=3;issue=3;spage=174;epage=177;aulast=Asmoro |
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