Macrophages derived from pluripotent stem cells: prospective applications and research gaps
Abstract Induced pluripotent stem cells (iPSCs) represent a valuable cell source able to give rise to different cell types of the body. Among the various pathways of iPSC differentiation, the differentiation into macrophages is a recently developed and rapidly growing technique. Macrophages play a k...
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| Format: | Article |
| Language: | English |
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BMC
2022-06-01
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| Series: | Cell & Bioscience |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s13578-022-00824-4 |
| _version_ | 1811230009340198912 |
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| author | Irina Lyadova Andrei Vasiliev |
| author_facet | Irina Lyadova Andrei Vasiliev |
| author_sort | Irina Lyadova |
| collection | DOAJ |
| description | Abstract Induced pluripotent stem cells (iPSCs) represent a valuable cell source able to give rise to different cell types of the body. Among the various pathways of iPSC differentiation, the differentiation into macrophages is a recently developed and rapidly growing technique. Macrophages play a key role in the control of host homeostasis. Their dysfunction underlies many diseases, including hereditary, infectious, oncological, metabolic and other disorders. Targeting macrophage activity and developing macrophage-based cell therapy represent promising tools for the treatment of many pathological conditions. Macrophages generated from human iPSCs (iMphs) provide great opportunities in these areas. The generation of iMphs is based on a step-wise differentiation of iPSCs into mesoderm, hematopoietic progenitors, myeloid monocyte-like cells and macrophages. The technique allows to obtain standardizable populations of human macrophages from any individual, scale up macrophage production and introduce genetic modifications, which gives significant advantages over the standard source of human macrophages, monocyte-derived macrophages. The spectrum of iMph applications is rapidly growing. iMphs have been successfully used to model hereditary diseases and macrophage-pathogen interactions, as well as to test drugs. iMph use for cell therapy is another promising and rapidly developing area of research. The principles and the details of iMph generation have recently been reviewed. This review systemizes current and prospective iMph applications and discusses the problem of iMph safety and other issues that need to be explored before iMphs become clinically applicable. |
| first_indexed | 2024-04-12T10:21:44Z |
| format | Article |
| id | doaj.art-a8e582b156eb4f769d4d915c5e5a1c4d |
| institution | Directory Open Access Journal |
| issn | 2045-3701 |
| language | English |
| last_indexed | 2024-04-12T10:21:44Z |
| publishDate | 2022-06-01 |
| publisher | BMC |
| record_format | Article |
| series | Cell & Bioscience |
| spelling | doaj.art-a8e582b156eb4f769d4d915c5e5a1c4d2022-12-22T03:37:04ZengBMCCell & Bioscience2045-37012022-06-0112112510.1186/s13578-022-00824-4Macrophages derived from pluripotent stem cells: prospective applications and research gapsIrina Lyadova0Andrei Vasiliev1Koltzov Institute of Developmental Biology of RASKoltzov Institute of Developmental Biology of RASAbstract Induced pluripotent stem cells (iPSCs) represent a valuable cell source able to give rise to different cell types of the body. Among the various pathways of iPSC differentiation, the differentiation into macrophages is a recently developed and rapidly growing technique. Macrophages play a key role in the control of host homeostasis. Their dysfunction underlies many diseases, including hereditary, infectious, oncological, metabolic and other disorders. Targeting macrophage activity and developing macrophage-based cell therapy represent promising tools for the treatment of many pathological conditions. Macrophages generated from human iPSCs (iMphs) provide great opportunities in these areas. The generation of iMphs is based on a step-wise differentiation of iPSCs into mesoderm, hematopoietic progenitors, myeloid monocyte-like cells and macrophages. The technique allows to obtain standardizable populations of human macrophages from any individual, scale up macrophage production and introduce genetic modifications, which gives significant advantages over the standard source of human macrophages, monocyte-derived macrophages. The spectrum of iMph applications is rapidly growing. iMphs have been successfully used to model hereditary diseases and macrophage-pathogen interactions, as well as to test drugs. iMph use for cell therapy is another promising and rapidly developing area of research. The principles and the details of iMph generation have recently been reviewed. This review systemizes current and prospective iMph applications and discusses the problem of iMph safety and other issues that need to be explored before iMphs become clinically applicable.https://doi.org/10.1186/s13578-022-00824-4Induced pluripotent stem cellsMacrophagesMacrophages derived from induced pluripotent stem cellsDisease modelingCell therapyHost–pathogen interactions |
| spellingShingle | Irina Lyadova Andrei Vasiliev Macrophages derived from pluripotent stem cells: prospective applications and research gaps Cell & Bioscience Induced pluripotent stem cells Macrophages Macrophages derived from induced pluripotent stem cells Disease modeling Cell therapy Host–pathogen interactions |
| title | Macrophages derived from pluripotent stem cells: prospective applications and research gaps |
| title_full | Macrophages derived from pluripotent stem cells: prospective applications and research gaps |
| title_fullStr | Macrophages derived from pluripotent stem cells: prospective applications and research gaps |
| title_full_unstemmed | Macrophages derived from pluripotent stem cells: prospective applications and research gaps |
| title_short | Macrophages derived from pluripotent stem cells: prospective applications and research gaps |
| title_sort | macrophages derived from pluripotent stem cells prospective applications and research gaps |
| topic | Induced pluripotent stem cells Macrophages Macrophages derived from induced pluripotent stem cells Disease modeling Cell therapy Host–pathogen interactions |
| url | https://doi.org/10.1186/s13578-022-00824-4 |
| work_keys_str_mv | AT irinalyadova macrophagesderivedfrompluripotentstemcellsprospectiveapplicationsandresearchgaps AT andreivasiliev macrophagesderivedfrompluripotentstemcellsprospectiveapplicationsandresearchgaps |