Optimization of Callus Induction and Cell Suspension in Catharanthus roseus
Periwinkle (<em>Catharanthus roseus</em>) has been considered as an important medicinal plant because it contains many alkaloids such as vincristine and vinblastine. <em>In vitro</em> culture of periwinkle provides new tissue sources such as callus, cell suspension and seedli...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | fas |
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Shahid Bahonar University of Kerman
2012-08-01
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| Series: | مجله بیوتکنولوژی کشاورزی |
| Subjects: | |
| Online Access: | https://jab.uk.ac.ir/article_464_28945cd483460db3af24b640ffa9b722.pdf |
| _version_ | 1819018951979433984 |
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| author | Jafar Ahmadi Razieh Mohammadi GHasemali Garoosi Ramin Hossini |
| author_facet | Jafar Ahmadi Razieh Mohammadi GHasemali Garoosi Ramin Hossini |
| author_sort | Jafar Ahmadi |
| collection | DOAJ |
| description | Periwinkle (<em>Catharanthus roseus</em>) has been considered as an important medicinal plant because it contains many alkaloids such as vincristine and vinblastine. <em>In vitro</em> culture of periwinkle provides new tissue sources such as callus, cell suspension and seedlings to produce secondary metabolites. As for the mass production of callus and cell suspension in order to optimize the extraction of secondary metabolites have been few studies, the present study describes two callus production optimization procedures and one cell suspension experiment. The first trail was a factorial experiment with three explants (roots, shoots and leaves) on Murashige and Skoog (MS) medium supplemented with different concentrations of BAP and 2,4-D. The second trial was a factorial experiment with six explants (roots, shoots, <em>in-vitro</em> grown leaves, <em>ex-vitro</em> grown leaves, seeds and nodes) and ten different hormonal combinations. The cell suspension experiment was conducted with six hormonal and one control (without hormone) treatments based on completely randomized design. Comparison of means showed that the maximum callus production was obtained from leaf explants, the root and shoot explants were in the second orders. In overall, 0.5 mg /l BAP + 1 mg/l 2,4-D and 0.5 mg /l KN + 1 mg/l 2,4-D concentrations proved to be optimal for the production of maximum callus. The best combination for suspension culture was 1 mg/l 2,4-D and/or 2 mg/l 2,4-D+1 mg /l BAP according to the dried cell weight |
| first_indexed | 2024-12-21T03:27:35Z |
| format | Article |
| id | doaj.art-a8e6b7a1e14b44bb85077e103340a15e |
| institution | Directory Open Access Journal |
| issn | 2228-6705 2228-6500 |
| language | fas |
| last_indexed | 2024-12-21T03:27:35Z |
| publishDate | 2012-08-01 |
| publisher | Shahid Bahonar University of Kerman |
| record_format | Article |
| series | مجله بیوتکنولوژی کشاورزی |
| spelling | doaj.art-a8e6b7a1e14b44bb85077e103340a15e2022-12-21T19:17:34ZfasShahid Bahonar University of Kermanمجله بیوتکنولوژی کشاورزی2228-67052228-65002012-08-014111810.22103/jab.2012.464464Optimization of Callus Induction and Cell Suspension in Catharanthus roseusJafar Ahmadi0Razieh Mohammadi1GHasemali Garoosi2Ramin Hossini3دانشیار دانشگاه بین المللی امام خمینی (ره) قزوینکارشناس ارشد دانشگاه بین المللی امام خمینی (ره) قزویناستادیار دانشگاه بین المللی امام خمینی (ره) قزویناستادیار دانشگاه بین المللی امام خمینی (ره) قزوینPeriwinkle (<em>Catharanthus roseus</em>) has been considered as an important medicinal plant because it contains many alkaloids such as vincristine and vinblastine. <em>In vitro</em> culture of periwinkle provides new tissue sources such as callus, cell suspension and seedlings to produce secondary metabolites. As for the mass production of callus and cell suspension in order to optimize the extraction of secondary metabolites have been few studies, the present study describes two callus production optimization procedures and one cell suspension experiment. The first trail was a factorial experiment with three explants (roots, shoots and leaves) on Murashige and Skoog (MS) medium supplemented with different concentrations of BAP and 2,4-D. The second trial was a factorial experiment with six explants (roots, shoots, <em>in-vitro</em> grown leaves, <em>ex-vitro</em> grown leaves, seeds and nodes) and ten different hormonal combinations. The cell suspension experiment was conducted with six hormonal and one control (without hormone) treatments based on completely randomized design. Comparison of means showed that the maximum callus production was obtained from leaf explants, the root and shoot explants were in the second orders. In overall, 0.5 mg /l BAP + 1 mg/l 2,4-D and 0.5 mg /l KN + 1 mg/l 2,4-D concentrations proved to be optimal for the production of maximum callus. The best combination for suspension culture was 1 mg/l 2,4-D and/or 2 mg/l 2,4-D+1 mg /l BAP according to the dried cell weighthttps://jab.uk.ac.ir/article_464_28945cd483460db3af24b640ffa9b722.pdfperiwinklecallus. suspension culturein vitro |
| spellingShingle | Jafar Ahmadi Razieh Mohammadi GHasemali Garoosi Ramin Hossini Optimization of Callus Induction and Cell Suspension in Catharanthus roseus مجله بیوتکنولوژی کشاورزی periwinkle callus. suspension culture in vitro |
| title | Optimization of Callus Induction and Cell Suspension in Catharanthus roseus |
| title_full | Optimization of Callus Induction and Cell Suspension in Catharanthus roseus |
| title_fullStr | Optimization of Callus Induction and Cell Suspension in Catharanthus roseus |
| title_full_unstemmed | Optimization of Callus Induction and Cell Suspension in Catharanthus roseus |
| title_short | Optimization of Callus Induction and Cell Suspension in Catharanthus roseus |
| title_sort | optimization of callus induction and cell suspension in catharanthus roseus |
| topic | periwinkle callus. suspension culture in vitro |
| url | https://jab.uk.ac.ir/article_464_28945cd483460db3af24b640ffa9b722.pdf |
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