Detection, Purification and Elucidation of Chemical Structure and Antiproliferative Activity of Taxol Produced by <em>Penicillium chrysogenum</em>
<i>Penicillium chrysogenum</i> has been reported as a potent taxol producer based on quantitative analysis by TLC and HPLC. The biosynthetic potency of taxol has been validated from PCR detection of rate-limiting genes of taxol synthesis such as taxadienesynthase and 10-de-acetylbaccatin...
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MDPI AG
2020-10-01
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| Series: | Molecules |
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| Online Access: | https://www.mdpi.com/1420-3049/25/20/4822 |
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| author | Ashraf El-Sayed Gamal Enan Abdul-Raouf Al-Mohammadi Ahmed H. Moustafa Nashwa El-Gazzar |
| author_facet | Ashraf El-Sayed Gamal Enan Abdul-Raouf Al-Mohammadi Ahmed H. Moustafa Nashwa El-Gazzar |
| author_sort | Ashraf El-Sayed |
| collection | DOAJ |
| description | <i>Penicillium chrysogenum</i> has been reported as a potent taxol producer based on quantitative analysis by TLC and HPLC. The biosynthetic potency of taxol has been validated from PCR detection of rate-limiting genes of taxol synthesis such as taxadienesynthase and 10-de-acetylbaccatin III-O-acetyltransferase (DBAT), which catalyzes the immediate diterpenoid precursor of the taxol substance, as detected by PCR. Taxol production by <i>P. chrysogenum</i> was assessed by growing the fungus on different media. Potato dextrose broth (PDB) was shown to be the best medium for obtaining the higher amount of taxol (170 µg/L). A stepwise optimization of culture conditions necessary for production of higher amounts of taxol was investigated. The substance taxol was produced optimally after 18 d of incubation at 30 °C in PDB adjusted initially at pH 8.0 with shaking (120 rpm) (250 µg/L). The <i>P. chrysogenum</i> taxol was purified successfully by HPLC. Instrumental analyzes such as Fourier transform infrared spectroscopy (FTIR), ultraviolet (UV) spectroscopy, <sup>1</sup>HNMR and <sup>13</sup>C NMR approved the structural formula of taxol (C<sub>47</sub>H<sub>51</sub>NO<sub>14</sub>), as constructed by ChemDraw. The <i>P. chrysogenum</i> taxol showed promising anticancer activity. |
| first_indexed | 2024-03-10T15:28:50Z |
| format | Article |
| id | doaj.art-a8e86c72469b4f109f9078f080120800 |
| institution | Directory Open Access Journal |
| issn | 1420-3049 |
| language | English |
| last_indexed | 2024-03-10T15:28:50Z |
| publishDate | 2020-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Molecules |
| spelling | doaj.art-a8e86c72469b4f109f9078f0801208002023-11-20T17:52:05ZengMDPI AGMolecules1420-30492020-10-012520482210.3390/molecules25204822Detection, Purification and Elucidation of Chemical Structure and Antiproliferative Activity of Taxol Produced by <em>Penicillium chrysogenum</em>Ashraf El-Sayed0Gamal Enan1Abdul-Raouf Al-Mohammadi2Ahmed H. Moustafa3Nashwa El-Gazzar4Botany and Microbiology Department, Faculty of Science, Zagazig University, Zagazig 44519, EgyptBotany and Microbiology Department, Faculty of Science, Zagazig University, Zagazig 44519, EgyptDepartment of Sciences, King Khalid Military Academy, Riyadh 11495, P.O. Box 22140, Saudi ArabiaChemistry Department, Faculty of Science, Zagazig University, Zagazig 44519, EgyptBotany and Microbiology Department, Faculty of Science, Zagazig University, Zagazig 44519, Egypt<i>Penicillium chrysogenum</i> has been reported as a potent taxol producer based on quantitative analysis by TLC and HPLC. The biosynthetic potency of taxol has been validated from PCR detection of rate-limiting genes of taxol synthesis such as taxadienesynthase and 10-de-acetylbaccatin III-O-acetyltransferase (DBAT), which catalyzes the immediate diterpenoid precursor of the taxol substance, as detected by PCR. Taxol production by <i>P. chrysogenum</i> was assessed by growing the fungus on different media. Potato dextrose broth (PDB) was shown to be the best medium for obtaining the higher amount of taxol (170 µg/L). A stepwise optimization of culture conditions necessary for production of higher amounts of taxol was investigated. The substance taxol was produced optimally after 18 d of incubation at 30 °C in PDB adjusted initially at pH 8.0 with shaking (120 rpm) (250 µg/L). The <i>P. chrysogenum</i> taxol was purified successfully by HPLC. Instrumental analyzes such as Fourier transform infrared spectroscopy (FTIR), ultraviolet (UV) spectroscopy, <sup>1</sup>HNMR and <sup>13</sup>C NMR approved the structural formula of taxol (C<sub>47</sub>H<sub>51</sub>NO<sub>14</sub>), as constructed by ChemDraw. The <i>P. chrysogenum</i> taxol showed promising anticancer activity.https://www.mdpi.com/1420-3049/25/20/4822<i>Penicillium chrysogenum</i>taxolinstrumental analysisantiproliferative activity |
| spellingShingle | Ashraf El-Sayed Gamal Enan Abdul-Raouf Al-Mohammadi Ahmed H. Moustafa Nashwa El-Gazzar Detection, Purification and Elucidation of Chemical Structure and Antiproliferative Activity of Taxol Produced by <em>Penicillium chrysogenum</em> Molecules <i>Penicillium chrysogenum</i> taxol instrumental analysis antiproliferative activity |
| title | Detection, Purification and Elucidation of Chemical Structure and Antiproliferative Activity of Taxol Produced by <em>Penicillium chrysogenum</em> |
| title_full | Detection, Purification and Elucidation of Chemical Structure and Antiproliferative Activity of Taxol Produced by <em>Penicillium chrysogenum</em> |
| title_fullStr | Detection, Purification and Elucidation of Chemical Structure and Antiproliferative Activity of Taxol Produced by <em>Penicillium chrysogenum</em> |
| title_full_unstemmed | Detection, Purification and Elucidation of Chemical Structure and Antiproliferative Activity of Taxol Produced by <em>Penicillium chrysogenum</em> |
| title_short | Detection, Purification and Elucidation of Chemical Structure and Antiproliferative Activity of Taxol Produced by <em>Penicillium chrysogenum</em> |
| title_sort | detection purification and elucidation of chemical structure and antiproliferative activity of taxol produced by em penicillium chrysogenum em |
| topic | <i>Penicillium chrysogenum</i> taxol instrumental analysis antiproliferative activity |
| url | https://www.mdpi.com/1420-3049/25/20/4822 |
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