New data analysis for BioSAXS at the ESRF
The second phase of the ESRF upgrade program did not only provide a new storage ring (Extremely Brilliant Source, EBS) but also allowed several beamlines to be refurbished. The BioSAXS beamline (located on port BM29) was upgraded with a new wiggler source and a larger detector. All analysis software...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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International Union of Crystallography
2022-09-01
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| Series: | Journal of Synchrotron Radiation |
| Subjects: | |
| Online Access: | http://scripts.iucr.org/cgi-bin/paper?S1600577522007238 |
| _version_ | 1828176324059987968 |
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| author | Jérôme Kieffer Martha Brennich Jean-Baptiste Florial Marcus Oscarsson Alejandro De Maria Antolinos Mark Tully Petra Pernot |
| author_facet | Jérôme Kieffer Martha Brennich Jean-Baptiste Florial Marcus Oscarsson Alejandro De Maria Antolinos Mark Tully Petra Pernot |
| author_sort | Jérôme Kieffer |
| collection | DOAJ |
| description | The second phase of the ESRF upgrade program did not only provide a new storage ring (Extremely Brilliant Source, EBS) but also allowed several beamlines to be refurbished. The BioSAXS beamline (located on port BM29) was upgraded with a new wiggler source and a larger detector. All analysis software has been rewritten to cope with the increased data flux and continues to provide beamline users with reduced and pre-processed data in real time. This article describes FreeSAS, an open-source collection of various small-angle scattering analysis algorithms needed to reduce and analyze BioSAXS data, and Dahu, the tool used to interface data analysis with beamline control. It further presents the data-processing pipelines for the different data acquisitions modes of the beamline, using either a sample changer for individual homogeneous samples or an inline size-exclusion chromatography setup. |
| first_indexed | 2024-04-12T04:41:32Z |
| format | Article |
| id | doaj.art-a8e8ef0ebc8b469c94217d5c4bc683b3 |
| institution | Directory Open Access Journal |
| issn | 1600-5775 |
| language | English |
| last_indexed | 2024-04-12T04:41:32Z |
| publishDate | 2022-09-01 |
| publisher | International Union of Crystallography |
| record_format | Article |
| series | Journal of Synchrotron Radiation |
| spelling | doaj.art-a8e8ef0ebc8b469c94217d5c4bc683b32022-12-22T03:47:37ZengInternational Union of CrystallographyJournal of Synchrotron Radiation1600-57752022-09-012951318132810.1107/S1600577522007238ju5045New data analysis for BioSAXS at the ESRFJérôme Kieffer0Martha Brennich1Jean-Baptiste Florial2Marcus Oscarsson3Alejandro De Maria Antolinos4Mark Tully5Petra Pernot6ESRF – The European Synchrotron, 71 Avenue des Martyrs, 38000 Grenoble, FranceEuropean Molecular Biology Laboratory, 71 Avenue des Martyrs, 38000 Grenoble, FranceEuropean Molecular Biology Laboratory, 71 Avenue des Martyrs, 38000 Grenoble, FranceESRF – The European Synchrotron, 71 Avenue des Martyrs, 38000 Grenoble, FranceESRF – The European Synchrotron, 71 Avenue des Martyrs, 38000 Grenoble, FranceESRF – The European Synchrotron, 71 Avenue des Martyrs, 38000 Grenoble, FranceESRF – The European Synchrotron, 71 Avenue des Martyrs, 38000 Grenoble, FranceThe second phase of the ESRF upgrade program did not only provide a new storage ring (Extremely Brilliant Source, EBS) but also allowed several beamlines to be refurbished. The BioSAXS beamline (located on port BM29) was upgraded with a new wiggler source and a larger detector. All analysis software has been rewritten to cope with the increased data flux and continues to provide beamline users with reduced and pre-processed data in real time. This article describes FreeSAS, an open-source collection of various small-angle scattering analysis algorithms needed to reduce and analyze BioSAXS data, and Dahu, the tool used to interface data analysis with beamline control. It further presents the data-processing pipelines for the different data acquisitions modes of the beamline, using either a sample changer for individual homogeneous samples or an inline size-exclusion chromatography setup.http://scripts.iucr.org/cgi-bin/paper?S1600577522007238biosaxsonline data analysissolution scatteringproteinsbiological small-angle x-ray scatteringautomationhigh brilliancestructural biologyhigh-throughput saxssize-exclusion chromatographyonline purification |
| spellingShingle | Jérôme Kieffer Martha Brennich Jean-Baptiste Florial Marcus Oscarsson Alejandro De Maria Antolinos Mark Tully Petra Pernot New data analysis for BioSAXS at the ESRF Journal of Synchrotron Radiation biosaxs online data analysis solution scattering proteins biological small-angle x-ray scattering automation high brilliance structural biology high-throughput saxs size-exclusion chromatography online purification |
| title | New data analysis for BioSAXS at the ESRF |
| title_full | New data analysis for BioSAXS at the ESRF |
| title_fullStr | New data analysis for BioSAXS at the ESRF |
| title_full_unstemmed | New data analysis for BioSAXS at the ESRF |
| title_short | New data analysis for BioSAXS at the ESRF |
| title_sort | new data analysis for biosaxs at the esrf |
| topic | biosaxs online data analysis solution scattering proteins biological small-angle x-ray scattering automation high brilliance structural biology high-throughput saxs size-exclusion chromatography online purification |
| url | http://scripts.iucr.org/cgi-bin/paper?S1600577522007238 |
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