Surface Modification Techniques for Endothelial Cell Seeding in PDMS Microfluidic Devices
Microfluidic lab-on-a-chip cell culture techniques have been gaining popularity by offering the possibility of reducing the amount of samples and reagents and greater control over cellular microenvironment. Polydimethylsiloxane (PDMS) is the commonly used polymer for microfluidic cell culture device...
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| Format: | Article |
| Language: | English |
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MDPI AG
2020-11-01
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| Series: | Biosensors |
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| Online Access: | https://www.mdpi.com/2079-6374/10/11/182 |
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| author | Fahima Akther Shazwani Binte Yakob Nam-Trung Nguyen Hang T. Ta |
| author_facet | Fahima Akther Shazwani Binte Yakob Nam-Trung Nguyen Hang T. Ta |
| author_sort | Fahima Akther |
| collection | DOAJ |
| description | Microfluidic lab-on-a-chip cell culture techniques have been gaining popularity by offering the possibility of reducing the amount of samples and reagents and greater control over cellular microenvironment. Polydimethylsiloxane (PDMS) is the commonly used polymer for microfluidic cell culture devices because of the cheap and easy fabrication techniques, non-toxicity, biocompatibility, high gas permeability, and optical transparency. However, the intrinsic hydrophobic nature of PDMS makes cell seeding challenging when applied on PDMS surface. The hydrophobicity of the PDMS surface also allows the non-specific absorption/adsorption of small molecules and biomolecules that might affect the cellular behaviour and functions. Hydrophilic modification of PDMS surface is indispensable for successful cell seeding. This review collates different techniques with their advantages and disadvantages that have been used to improve PDMS hydrophilicity to facilitate endothelial cells seeding in PDMS devices. |
| first_indexed | 2024-03-10T14:44:06Z |
| format | Article |
| id | doaj.art-a8ea67936ef04bf88d92b7853a62e332 |
| institution | Directory Open Access Journal |
| issn | 2079-6374 |
| language | English |
| last_indexed | 2024-03-10T14:44:06Z |
| publishDate | 2020-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Biosensors |
| spelling | doaj.art-a8ea67936ef04bf88d92b7853a62e3322023-11-20T21:32:00ZengMDPI AGBiosensors2079-63742020-11-01101118210.3390/bios10110182Surface Modification Techniques for Endothelial Cell Seeding in PDMS Microfluidic DevicesFahima Akther0Shazwani Binte Yakob1Nam-Trung Nguyen2Hang T. Ta3Australian Institute for Bioengineering and Nanotechnology, the University of Queensland, Brisbane, QLD 4072, AustraliaSchool of Pharmacy, the University of Queensland, Brisbane, QLD 4102, AustraliaQueensland Micro-and Nanotechnology Centre, Griffith University, Brisbane, QLD 4111, AustraliaAustralian Institute for Bioengineering and Nanotechnology, the University of Queensland, Brisbane, QLD 4072, AustraliaMicrofluidic lab-on-a-chip cell culture techniques have been gaining popularity by offering the possibility of reducing the amount of samples and reagents and greater control over cellular microenvironment. Polydimethylsiloxane (PDMS) is the commonly used polymer for microfluidic cell culture devices because of the cheap and easy fabrication techniques, non-toxicity, biocompatibility, high gas permeability, and optical transparency. However, the intrinsic hydrophobic nature of PDMS makes cell seeding challenging when applied on PDMS surface. The hydrophobicity of the PDMS surface also allows the non-specific absorption/adsorption of small molecules and biomolecules that might affect the cellular behaviour and functions. Hydrophilic modification of PDMS surface is indispensable for successful cell seeding. This review collates different techniques with their advantages and disadvantages that have been used to improve PDMS hydrophilicity to facilitate endothelial cells seeding in PDMS devices.https://www.mdpi.com/2079-6374/10/11/182PDMSmicrofluidicsendothelial cellssurface treatmenthydrophobicity |
| spellingShingle | Fahima Akther Shazwani Binte Yakob Nam-Trung Nguyen Hang T. Ta Surface Modification Techniques for Endothelial Cell Seeding in PDMS Microfluidic Devices Biosensors PDMS microfluidics endothelial cells surface treatment hydrophobicity |
| title | Surface Modification Techniques for Endothelial Cell Seeding in PDMS Microfluidic Devices |
| title_full | Surface Modification Techniques for Endothelial Cell Seeding in PDMS Microfluidic Devices |
| title_fullStr | Surface Modification Techniques for Endothelial Cell Seeding in PDMS Microfluidic Devices |
| title_full_unstemmed | Surface Modification Techniques for Endothelial Cell Seeding in PDMS Microfluidic Devices |
| title_short | Surface Modification Techniques for Endothelial Cell Seeding in PDMS Microfluidic Devices |
| title_sort | surface modification techniques for endothelial cell seeding in pdms microfluidic devices |
| topic | PDMS microfluidics endothelial cells surface treatment hydrophobicity |
| url | https://www.mdpi.com/2079-6374/10/11/182 |
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