In vitro antimalarial activity of Calophyllum bicolor and hemozoin crystals observed by Transmission Electron Microscope (TEM)
Objective : In continuation of our antimalarial candidate drug discovery program on Indonesia medicinal plants especially from stem bark of Calophyllum bicolor. Metode : We extracted of bioactive crude extract with hexane, acetone and methanol from stem bark of Calophyllum bicolor and evaluated thei...
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| Format: | Article |
| Language: | English |
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EDP Sciences
2018-01-01
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| Series: | MATEC Web of Conferences |
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| Online Access: | https://doi.org/10.1051/matecconf/201815404004 |
| _version_ | 1818338613982658560 |
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| author | Abbas Jamilah |
| author_facet | Abbas Jamilah |
| author_sort | Abbas Jamilah |
| collection | DOAJ |
| description | Objective : In continuation of our antimalarial candidate drug discovery program on Indonesia medicinal plants especially from stem bark of Calophyllum bicolor. Metode : We extracted of bioactive crude extract with hexane, acetone and methanol from stem bark of Calophyllum bicolor and evaluated their antimalarial activity by using parasite Plasmodium falciparum in vitro. Results: Methanol fraction showed most active and potent antimalarial activity dose dependent in in vitro experiments with IC50 = 0.52 ppb, and hexane fraction showed moderate antimalarial activity, but dose not dependent, while acetone fraction have not antimalarial activity. TEM (Trasmission Electron Microscopy) analysis confirmed a remarkable reduction of hemozoin in the presence of the bioactive fraction. Conclusion: The results suggest that the antimalarial activity of Calophyllum bicolor is due, methanol fraction showed the best in vitro antimalarial activity then another fraction. |
| first_indexed | 2024-12-13T15:13:54Z |
| format | Article |
| id | doaj.art-a8ebc4650883436587f4fb3d0c8a9999 |
| institution | Directory Open Access Journal |
| issn | 2261-236X |
| language | English |
| last_indexed | 2024-12-13T15:13:54Z |
| publishDate | 2018-01-01 |
| publisher | EDP Sciences |
| record_format | Article |
| series | MATEC Web of Conferences |
| spelling | doaj.art-a8ebc4650883436587f4fb3d0c8a99992022-12-21T23:40:46ZengEDP SciencesMATEC Web of Conferences2261-236X2018-01-011540400410.1051/matecconf/201815404004matecconf_icet4sd2018_04004In vitro antimalarial activity of Calophyllum bicolor and hemozoin crystals observed by Transmission Electron Microscope (TEM)Abbas JamilahObjective : In continuation of our antimalarial candidate drug discovery program on Indonesia medicinal plants especially from stem bark of Calophyllum bicolor. Metode : We extracted of bioactive crude extract with hexane, acetone and methanol from stem bark of Calophyllum bicolor and evaluated their antimalarial activity by using parasite Plasmodium falciparum in vitro. Results: Methanol fraction showed most active and potent antimalarial activity dose dependent in in vitro experiments with IC50 = 0.52 ppb, and hexane fraction showed moderate antimalarial activity, but dose not dependent, while acetone fraction have not antimalarial activity. TEM (Trasmission Electron Microscopy) analysis confirmed a remarkable reduction of hemozoin in the presence of the bioactive fraction. Conclusion: The results suggest that the antimalarial activity of Calophyllum bicolor is due, methanol fraction showed the best in vitro antimalarial activity then another fraction.https://doi.org/10.1051/matecconf/201815404004Calophyllum bicolorPlasmodium falciparumin-vitroTEM (Transmition Electron Mecroscope) |
| spellingShingle | Abbas Jamilah In vitro antimalarial activity of Calophyllum bicolor and hemozoin crystals observed by Transmission Electron Microscope (TEM) MATEC Web of Conferences Calophyllum bicolor Plasmodium falciparum in-vitro TEM (Transmition Electron Mecroscope) |
| title | In vitro antimalarial activity of Calophyllum bicolor and hemozoin crystals observed by Transmission Electron Microscope (TEM) |
| title_full | In vitro antimalarial activity of Calophyllum bicolor and hemozoin crystals observed by Transmission Electron Microscope (TEM) |
| title_fullStr | In vitro antimalarial activity of Calophyllum bicolor and hemozoin crystals observed by Transmission Electron Microscope (TEM) |
| title_full_unstemmed | In vitro antimalarial activity of Calophyllum bicolor and hemozoin crystals observed by Transmission Electron Microscope (TEM) |
| title_short | In vitro antimalarial activity of Calophyllum bicolor and hemozoin crystals observed by Transmission Electron Microscope (TEM) |
| title_sort | in vitro antimalarial activity of calophyllum bicolor and hemozoin crystals observed by transmission electron microscope tem |
| topic | Calophyllum bicolor Plasmodium falciparum in-vitro TEM (Transmition Electron Mecroscope) |
| url | https://doi.org/10.1051/matecconf/201815404004 |
| work_keys_str_mv | AT abbasjamilah invitroantimalarialactivityofcalophyllumbicolorandhemozoincrystalsobservedbytransmissionelectronmicroscopetem |