Comparative analysis of hepatic transcriptomes and metabolomes of Changshun green-shell laying hens based on different green eggshell color intensities
ABSTRACT: The eggshell color of avian species is an important trait that is predominantly determined by the pigments biliverdin and protoporphyrin. Various factors affect eggshell pigment deposition and coloration; however, the underlying mechanisms remain unclear. We analyzed the hepatic transcript...
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| Format: | Article |
| Language: | English |
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Elsevier
2024-01-01
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| Series: | Poultry Science |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S0032579123007393 |
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| author | Wenbin Xu Ren Mu Tuya Gegen Jiaxiang Luo Yang Xiao Shunnian Ou Qi Wu Yongsong Zuo Zhi Chen Fangwei Li |
| author_facet | Wenbin Xu Ren Mu Tuya Gegen Jiaxiang Luo Yang Xiao Shunnian Ou Qi Wu Yongsong Zuo Zhi Chen Fangwei Li |
| author_sort | Wenbin Xu |
| collection | DOAJ |
| description | ABSTRACT: The eggshell color of avian species is an important trait that is predominantly determined by the pigments biliverdin and protoporphyrin. Various factors affect eggshell pigment deposition and coloration; however, the underlying mechanisms remain unclear. We analyzed the hepatic transcriptomes and metabolomes of Changshun green-shell hens laying dark green and light green eggs to investigate the potential role of the liver in regulating the intensity of the green eggshell color. In total, 350 differentially expressed genes and 211 differentially altered metabolites were identified. Gene set enrichment analysis revealed that the enriched pathways and Gene Ontology (GO) terms were mainly associated with energy, immunity, and nutrient metabolism. Metabolite set enrichment analysis revealed that the enriched pathways were mainly associated with amino acid, vitamin, bile acid, and lipid metabolism. Moreover, gene–metabolite interaction network analysis revealed 1 subnetwork. Most genes and metabolites in this subnetwork were determined to be related to melanin metabolism and transport. In conclusion, our results suggest that hepatic melanin metabolism and transport are critical for eggshell coloration. Six candidate genes (CDKN2B, DDC, PYCR1, ABCG5, SLC3A1, and P2RX2) and 7 candidate metabolites (serotonin, 5-hydroxyindoleacetic acid, ornithine, acetylcholine, L-tryptophan, D-ornithine, and ADP) were suggested to play important roles in this process. Meanwhile, this study suggests that changes in hepatic energy metabolism, immune status, antioxidation activity, nutrient availability, and bile acid synthesis can impair eggshell coloration. |
| first_indexed | 2024-03-08T17:07:54Z |
| format | Article |
| id | doaj.art-a8ecd82ba40943de8064b368185c5032 |
| institution | Directory Open Access Journal |
| issn | 0032-5791 |
| language | English |
| last_indexed | 2024-03-08T17:07:54Z |
| publishDate | 2024-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Poultry Science |
| spelling | doaj.art-a8ecd82ba40943de8064b368185c50322024-01-04T04:35:40ZengElsevierPoultry Science0032-57912024-01-011031103220Comparative analysis of hepatic transcriptomes and metabolomes of Changshun green-shell laying hens based on different green eggshell color intensitiesWenbin Xu0Ren Mu1Tuya Gegen2Jiaxiang Luo3Yang Xiao4Shunnian Ou5Qi Wu6Yongsong Zuo7Zhi Chen8Fangwei Li9College of Biological Science and Agriculture, Qiannan Normal University for Nationalities, Duyun 558000, China; Qiannan Key Laboratory of Applied Biotechnology for Livestock and Poultry, Duyun 558000, China; College of Animal Sciences, Zhejiang University, Hangzhou 310058, ChinaCollege of Biological Science and Agriculture, Qiannan Normal University for Nationalities, Duyun 558000, China; Qiannan Key Laboratory of Applied Biotechnology for Livestock and Poultry, Duyun 558000, China; Corresponding author:Qiannan Key Laboratory of Applied Biotechnology for Livestock and Poultry, Duyun 558000, China; Library, Qiannan Normal University for Nationalities, Duyun 558000, ChinaCollege of Biological Science and Agriculture, Qiannan Normal University for Nationalities, Duyun 558000, ChinaCollege of Biological Science and Agriculture, Qiannan Normal University for Nationalities, Duyun 558000, ChinaCollege of Biological Science and Agriculture, Qiannan Normal University for Nationalities, Duyun 558000, ChinaCollege of Biological Science and Agriculture, Qiannan Normal University for Nationalities, Duyun 558000, China; Qiannan Key Laboratory of Applied Biotechnology for Livestock and Poultry, Duyun 558000, ChinaCollege of Biological Science and Agriculture, Qiannan Normal University for Nationalities, Duyun 558000, China; Qiannan Key Laboratory of Applied Biotechnology for Livestock and Poultry, Duyun 558000, ChinaCollege of Biological Science and Agriculture, Qiannan Normal University for Nationalities, Duyun 558000, China; Qiannan Key Laboratory of Applied Biotechnology for Livestock and Poultry, Duyun 558000, ChinaGuizhou Changshun Tiannong Green Shell Laying Hen Industrial Co. Ltd., Chang Shun 550700, ChinaABSTRACT: The eggshell color of avian species is an important trait that is predominantly determined by the pigments biliverdin and protoporphyrin. Various factors affect eggshell pigment deposition and coloration; however, the underlying mechanisms remain unclear. We analyzed the hepatic transcriptomes and metabolomes of Changshun green-shell hens laying dark green and light green eggs to investigate the potential role of the liver in regulating the intensity of the green eggshell color. In total, 350 differentially expressed genes and 211 differentially altered metabolites were identified. Gene set enrichment analysis revealed that the enriched pathways and Gene Ontology (GO) terms were mainly associated with energy, immunity, and nutrient metabolism. Metabolite set enrichment analysis revealed that the enriched pathways were mainly associated with amino acid, vitamin, bile acid, and lipid metabolism. Moreover, gene–metabolite interaction network analysis revealed 1 subnetwork. Most genes and metabolites in this subnetwork were determined to be related to melanin metabolism and transport. In conclusion, our results suggest that hepatic melanin metabolism and transport are critical for eggshell coloration. Six candidate genes (CDKN2B, DDC, PYCR1, ABCG5, SLC3A1, and P2RX2) and 7 candidate metabolites (serotonin, 5-hydroxyindoleacetic acid, ornithine, acetylcholine, L-tryptophan, D-ornithine, and ADP) were suggested to play important roles in this process. Meanwhile, this study suggests that changes in hepatic energy metabolism, immune status, antioxidation activity, nutrient availability, and bile acid synthesis can impair eggshell coloration.http://www.sciencedirect.com/science/article/pii/S0032579123007393Changshun green-shell laying henseggshell colorationbiliverdintranscriptome analysismetabolome analysis |
| spellingShingle | Wenbin Xu Ren Mu Tuya Gegen Jiaxiang Luo Yang Xiao Shunnian Ou Qi Wu Yongsong Zuo Zhi Chen Fangwei Li Comparative analysis of hepatic transcriptomes and metabolomes of Changshun green-shell laying hens based on different green eggshell color intensities Poultry Science Changshun green-shell laying hens eggshell coloration biliverdin transcriptome analysis metabolome analysis |
| title | Comparative analysis of hepatic transcriptomes and metabolomes of Changshun green-shell laying hens based on different green eggshell color intensities |
| title_full | Comparative analysis of hepatic transcriptomes and metabolomes of Changshun green-shell laying hens based on different green eggshell color intensities |
| title_fullStr | Comparative analysis of hepatic transcriptomes and metabolomes of Changshun green-shell laying hens based on different green eggshell color intensities |
| title_full_unstemmed | Comparative analysis of hepatic transcriptomes and metabolomes of Changshun green-shell laying hens based on different green eggshell color intensities |
| title_short | Comparative analysis of hepatic transcriptomes and metabolomes of Changshun green-shell laying hens based on different green eggshell color intensities |
| title_sort | comparative analysis of hepatic transcriptomes and metabolomes of changshun green shell laying hens based on different green eggshell color intensities |
| topic | Changshun green-shell laying hens eggshell coloration biliverdin transcriptome analysis metabolome analysis |
| url | http://www.sciencedirect.com/science/article/pii/S0032579123007393 |
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