The Establishment of Quantitatively Regulating Expression Cassette with sgRNA Targeting BIRC5 to Elucidate the Synergistic Pathway of Survivin with P-Glycoprotein in Cancer Multi-Drug Resistance

The Establishment of Quantitatively Regulating Expression Cassette with sgRNA Targeting BIRC5 to Elucidate the Synergistic Pathway of Survivin with P-Glycoprotein in Cancer Multi-Drug Resistance

Quantitative analysis and regulating gene expression in cancer cells is an innovative method to study key genes in tumors, which conduces to analyze the biological function of the specific gene. In this study, we found the expression levels of Survivin protein (BIRC5) and P-glycoprotein (MDR1) in MC...

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Bibliographic Details
Main Authors: Changping Deng, Fabiao Hu, Zhangting Zhao, Yiwen Zhou, Yuping Liu, Tong Zhang, Shihui Li, Wenyun Zheng, Wenliang Zhang, Tianwen Wang, Xingyuan Ma
Format: Article
Language:English
Published: Frontiers Media S.A. 2022-01-01
Series:Frontiers in Cell and Developmental Biology
Subjects:
survivin
P-Glycoprotein
CRISPR/Cas9-based Tet-off system
PI3K/Akt/mTOR pathway
MCF-7 cells
multi-drug resistance
Online Access:https://www.frontiersin.org/articles/10.3389/fcell.2021.797005/full

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