Prenatal caloric restriction alters lipid metabolism but not hepatic Fasn gene expression and methylation profiles in rats
Abstract Background Undernutrition is an increasingly common problem. Insufficient calorie intake and nutrient deficiencies during pregnancy may have an impact not only on the mother, but may also alter metabolism in the infant. In this study, we have applied a calorie-restricted diet during gestati...
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| Format: | Article |
| Language: | English |
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BMC
2017-08-01
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| Series: | BMC Genetics |
| Subjects: | |
| Online Access: | http://link.springer.com/article/10.1186/s12863-017-0544-0 |
| _version_ | 1818007325521215488 |
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| author | Joanna Nowacka-Woszuk Zofia E. Madeja Agata Chmurzynska |
| author_facet | Joanna Nowacka-Woszuk Zofia E. Madeja Agata Chmurzynska |
| author_sort | Joanna Nowacka-Woszuk |
| collection | DOAJ |
| description | Abstract Background Undernutrition is an increasingly common problem. Insufficient calorie intake and nutrient deficiencies during pregnancy may have an impact not only on the mother, but may also alter metabolism in the infant. In this study, we have applied a calorie-restricted diet during gestation and examined its effect on hepatic Fasn mRNA and DNA methylation profiles in rats and their female progeny. The body composition and blood lipid profiles were also evaluated in both generations. Results The results showed that the investigated diet regimen exerted a greater effect on the dams than on the offspring. We found that, in the calorie-restricted group, the transcript level of the Fasn gene in the liver increased in the mothers, while in the progeny it was only slightly enhanced. The implemented diet altered lipid profile in the dams by decreasing total cholesterol, HDL, and TG levels. An increase in LDL was noted in the offspring. No change in DNA methylation profile was observed in response to the calorie-restricted diet. Conclusions Calorie restriction during pregnancy modified the hepatic Fasn mRNA transcript level and altered the blood cholesterol concentrations in dams, but there were no such effects in their four-week-old offspring. The examined dietary regimen had no effect on DNA methylation of the Fasn 5′-flanking region in the rat liver. |
| first_indexed | 2024-04-14T05:13:51Z |
| format | Article |
| id | doaj.art-a8ed27bc4ad748fe8be038ef6d13ea42 |
| institution | Directory Open Access Journal |
| issn | 1471-2156 |
| language | English |
| last_indexed | 2024-04-14T05:13:51Z |
| publishDate | 2017-08-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Genetics |
| spelling | doaj.art-a8ed27bc4ad748fe8be038ef6d13ea422022-12-22T02:10:27ZengBMCBMC Genetics1471-21562017-08-011811810.1186/s12863-017-0544-0Prenatal caloric restriction alters lipid metabolism but not hepatic Fasn gene expression and methylation profiles in ratsJoanna Nowacka-Woszuk0Zofia E. Madeja1Agata Chmurzynska2Department of Genetics and Animal Breeding, Poznań University of Life SciencesDepartment of Genetics and Animal Breeding, Poznań University of Life SciencesDepartment of Human Nutrition and Hygiene, Poznań University of Life SciencesAbstract Background Undernutrition is an increasingly common problem. Insufficient calorie intake and nutrient deficiencies during pregnancy may have an impact not only on the mother, but may also alter metabolism in the infant. In this study, we have applied a calorie-restricted diet during gestation and examined its effect on hepatic Fasn mRNA and DNA methylation profiles in rats and their female progeny. The body composition and blood lipid profiles were also evaluated in both generations. Results The results showed that the investigated diet regimen exerted a greater effect on the dams than on the offspring. We found that, in the calorie-restricted group, the transcript level of the Fasn gene in the liver increased in the mothers, while in the progeny it was only slightly enhanced. The implemented diet altered lipid profile in the dams by decreasing total cholesterol, HDL, and TG levels. An increase in LDL was noted in the offspring. No change in DNA methylation profile was observed in response to the calorie-restricted diet. Conclusions Calorie restriction during pregnancy modified the hepatic Fasn mRNA transcript level and altered the blood cholesterol concentrations in dams, but there were no such effects in their four-week-old offspring. The examined dietary regimen had no effect on DNA methylation of the Fasn 5′-flanking region in the rat liver.http://link.springer.com/article/10.1186/s12863-017-0544-0NutrigenomicsCalorie-restricted dietRatLiverFasnGene expression |
| spellingShingle | Joanna Nowacka-Woszuk Zofia E. Madeja Agata Chmurzynska Prenatal caloric restriction alters lipid metabolism but not hepatic Fasn gene expression and methylation profiles in rats BMC Genetics Nutrigenomics Calorie-restricted diet Rat Liver Fasn Gene expression |
| title | Prenatal caloric restriction alters lipid metabolism but not hepatic Fasn gene expression and methylation profiles in rats |
| title_full | Prenatal caloric restriction alters lipid metabolism but not hepatic Fasn gene expression and methylation profiles in rats |
| title_fullStr | Prenatal caloric restriction alters lipid metabolism but not hepatic Fasn gene expression and methylation profiles in rats |
| title_full_unstemmed | Prenatal caloric restriction alters lipid metabolism but not hepatic Fasn gene expression and methylation profiles in rats |
| title_short | Prenatal caloric restriction alters lipid metabolism but not hepatic Fasn gene expression and methylation profiles in rats |
| title_sort | prenatal caloric restriction alters lipid metabolism but not hepatic fasn gene expression and methylation profiles in rats |
| topic | Nutrigenomics Calorie-restricted diet Rat Liver Fasn Gene expression |
| url | http://link.springer.com/article/10.1186/s12863-017-0544-0 |
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