PEMURNIAN PARSIAL DAN KRISTALISASI PAPAIN DARI GETAH Carica papaya
Abstract This research has done partial purification by fractionation and optimization crystallization of papain from Carica papaya latex with the addition of ammonium sulphate. The enhancement of purification factor was determined by its specific activity. The fractionation results show that papai...
| Main Authors: | , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Universitas Airlangga
2019-12-01
|
| Series: | Jurnal Kimia Riset |
| Online Access: | https://e-journal.unair.ac.id/JKR/article/view/16902 |
| _version_ | 1811213515093966848 |
|---|---|
| author | Dwi Putri Mashfufatur Rohmah Sofijan Hadi Afaf Baktir |
| author_facet | Dwi Putri Mashfufatur Rohmah Sofijan Hadi Afaf Baktir |
| author_sort | Dwi Putri Mashfufatur Rohmah |
| collection | DOAJ |
| description | Abstract
This research has done partial purification by fractionation and optimization crystallization of papain from Carica papaya latex with the addition of ammonium sulphate. The enhancement of purification factor was determined by its specific activity. The fractionation results show that papain fraction of Carica papaya can be obtained by adding 40-80% saturated ammonium sulphate, with the highest specific activity, i.e. 2,0819 U/µg and the factor purification increase of 6,27 fold than papain extract. Meanwhile, the highest total activity, i.e. 10,7780 U of papain crystals can be obtained by presipitant agent addition of ammonium sulphate in the level / concentration 80% saturated at 15 °C. Microscopycally papain crystals profile in this condition have cube and tetragonal shape.
Key words: crystallization, fractionation, ammonium sulphate, papain |
| first_indexed | 2024-04-12T05:47:52Z |
| format | Article |
| id | doaj.art-a8ef4182c75b4d31bf51366e9bc589ef |
| institution | Directory Open Access Journal |
| issn | 2528-0414 2528-0422 |
| language | English |
| last_indexed | 2024-04-12T05:47:52Z |
| publishDate | 2019-12-01 |
| publisher | Universitas Airlangga |
| record_format | Article |
| series | Jurnal Kimia Riset |
| spelling | doaj.art-a8ef4182c75b4d31bf51366e9bc589ef2022-12-22T03:45:24ZengUniversitas AirlanggaJurnal Kimia Riset2528-04142528-04222019-12-014215216010.20473/jkr.v4i2.1690213806PEMURNIAN PARSIAL DAN KRISTALISASI PAPAIN DARI GETAH Carica papayaDwi Putri Mashfufatur Rohmah0Sofijan Hadi1Afaf Baktir2Departemen Kimia, Fakultas Sains dan Teknologi Universitas Airlangga, SurabayaDepartemen Kimia, Fakultas Sains dan Teknologi Universitas Airlangga, SurabayaDepartemen Kimia, Fakultas Sains dan Teknologi Universitas Airlangga, SurabayaAbstract This research has done partial purification by fractionation and optimization crystallization of papain from Carica papaya latex with the addition of ammonium sulphate. The enhancement of purification factor was determined by its specific activity. The fractionation results show that papain fraction of Carica papaya can be obtained by adding 40-80% saturated ammonium sulphate, with the highest specific activity, i.e. 2,0819 U/µg and the factor purification increase of 6,27 fold than papain extract. Meanwhile, the highest total activity, i.e. 10,7780 U of papain crystals can be obtained by presipitant agent addition of ammonium sulphate in the level / concentration 80% saturated at 15 °C. Microscopycally papain crystals profile in this condition have cube and tetragonal shape. Key words: crystallization, fractionation, ammonium sulphate, papainhttps://e-journal.unair.ac.id/JKR/article/view/16902 |
| spellingShingle | Dwi Putri Mashfufatur Rohmah Sofijan Hadi Afaf Baktir PEMURNIAN PARSIAL DAN KRISTALISASI PAPAIN DARI GETAH Carica papaya Jurnal Kimia Riset |
| title | PEMURNIAN PARSIAL DAN KRISTALISASI PAPAIN DARI GETAH Carica papaya |
| title_full | PEMURNIAN PARSIAL DAN KRISTALISASI PAPAIN DARI GETAH Carica papaya |
| title_fullStr | PEMURNIAN PARSIAL DAN KRISTALISASI PAPAIN DARI GETAH Carica papaya |
| title_full_unstemmed | PEMURNIAN PARSIAL DAN KRISTALISASI PAPAIN DARI GETAH Carica papaya |
| title_short | PEMURNIAN PARSIAL DAN KRISTALISASI PAPAIN DARI GETAH Carica papaya |
| title_sort | pemurnian parsial dan kristalisasi papain dari getah carica papaya |
| url | https://e-journal.unair.ac.id/JKR/article/view/16902 |
| work_keys_str_mv | AT dwiputrimashfufaturrohmah pemurnianparsialdankristalisasipapaindarigetahcaricapapaya AT sofijanhadi pemurnianparsialdankristalisasipapaindarigetahcaricapapaya AT afafbaktir pemurnianparsialdankristalisasipapaindarigetahcaricapapaya |