Salivary miR-31-5p, miR-345-3p, and miR-424-3p Are Reliable Biomarkers in Patients with Oral Squamous Cell Carcinoma
If not detected early, oral squamous cell carcinoma (OSCC) has very poor prognosis, emphasizing the need for reliable early diagnostics. Saliva is considered a promising surrogate biosample for OSCC detection, because it comes into contact with many cells of the tumor mass, providing a comprehensive...
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-02-01
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| Series: | Pathogens |
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| Online Access: | https://www.mdpi.com/2076-0817/11/2/229 |
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| author | Beáta Scholtz József Horváth Ildikó Tar Csongor Kiss Ildikó J. Márton |
| author_facet | Beáta Scholtz József Horváth Ildikó Tar Csongor Kiss Ildikó J. Márton |
| author_sort | Beáta Scholtz |
| collection | DOAJ |
| description | If not detected early, oral squamous cell carcinoma (OSCC) has very poor prognosis, emphasizing the need for reliable early diagnostics. Saliva is considered a promising surrogate biosample for OSCC detection, because it comes into contact with many cells of the tumor mass, providing a comprehensive sampling of tumor-specific biomolecules. Although several protein- and RNA-based salivary biomarkers have been proposed for the detection of OSCC, the results of the studies show large differences. Our goal was to clarify which salivary microRNAs (miRNA) show reliably high expression in the saliva of OSCC patients, to be used as cancer-specific biomarkers, and potentially as early diagnostic biomarkers. Based on a detailed literature search, we selected six miRNAs commonly overexpressed in OSCC, and analyzed their expression in saliva samples of cancer patients and controls by real-time quantitative PCR. Our results suggest that miR-345 and miR-31-5p are consistently upregulated salivary biomarkers for OSCC, and a three-miRNA panel of miR-345, miR-31-5p, and miR-424-3p can distinguish cancer and control patients with high sensitivity. |
| first_indexed | 2024-03-09T21:17:09Z |
| format | Article |
| id | doaj.art-a8f001a78b174722b7d8062eaffc1757 |
| institution | Directory Open Access Journal |
| issn | 2076-0817 |
| language | English |
| last_indexed | 2024-03-09T21:17:09Z |
| publishDate | 2022-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Pathogens |
| spelling | doaj.art-a8f001a78b174722b7d8062eaffc17572023-11-23T21:32:22ZengMDPI AGPathogens2076-08172022-02-0111222910.3390/pathogens11020229Salivary miR-31-5p, miR-345-3p, and miR-424-3p Are Reliable Biomarkers in Patients with Oral Squamous Cell CarcinomaBeáta Scholtz0József Horváth1Ildikó Tar2Csongor Kiss3Ildikó J. Márton4Department of Biochemistry and Molecular Biology, Faculty of Medicine, University of Debrecen, 4032 Debrecen, HungaryDoctoral School of Molecular Cell and Immune Biology, University of Debrecen, 4032 Debrecen, HungaryDepartment of Oral Medicine, Faculty of Dentistry, University of Debrecen, 4032 Debrecen, HungaryDepartment of Pediatrics, Faculty of Medicine, University of Debrecen, 4032 Debrecen, HungaryDepartment of Restorative Dentistry, Faculty of Dentistry, University of Debrecen, 4032 Debrecen, HungaryIf not detected early, oral squamous cell carcinoma (OSCC) has very poor prognosis, emphasizing the need for reliable early diagnostics. Saliva is considered a promising surrogate biosample for OSCC detection, because it comes into contact with many cells of the tumor mass, providing a comprehensive sampling of tumor-specific biomolecules. Although several protein- and RNA-based salivary biomarkers have been proposed for the detection of OSCC, the results of the studies show large differences. Our goal was to clarify which salivary microRNAs (miRNA) show reliably high expression in the saliva of OSCC patients, to be used as cancer-specific biomarkers, and potentially as early diagnostic biomarkers. Based on a detailed literature search, we selected six miRNAs commonly overexpressed in OSCC, and analyzed their expression in saliva samples of cancer patients and controls by real-time quantitative PCR. Our results suggest that miR-345 and miR-31-5p are consistently upregulated salivary biomarkers for OSCC, and a three-miRNA panel of miR-345, miR-31-5p, and miR-424-3p can distinguish cancer and control patients with high sensitivity.https://www.mdpi.com/2076-0817/11/2/229oral squamous cell carcinomasalivamiR-345miR-31miR-424real-time qPCR |
| spellingShingle | Beáta Scholtz József Horváth Ildikó Tar Csongor Kiss Ildikó J. Márton Salivary miR-31-5p, miR-345-3p, and miR-424-3p Are Reliable Biomarkers in Patients with Oral Squamous Cell Carcinoma Pathogens oral squamous cell carcinoma saliva miR-345 miR-31 miR-424 real-time qPCR |
| title | Salivary miR-31-5p, miR-345-3p, and miR-424-3p Are Reliable Biomarkers in Patients with Oral Squamous Cell Carcinoma |
| title_full | Salivary miR-31-5p, miR-345-3p, and miR-424-3p Are Reliable Biomarkers in Patients with Oral Squamous Cell Carcinoma |
| title_fullStr | Salivary miR-31-5p, miR-345-3p, and miR-424-3p Are Reliable Biomarkers in Patients with Oral Squamous Cell Carcinoma |
| title_full_unstemmed | Salivary miR-31-5p, miR-345-3p, and miR-424-3p Are Reliable Biomarkers in Patients with Oral Squamous Cell Carcinoma |
| title_short | Salivary miR-31-5p, miR-345-3p, and miR-424-3p Are Reliable Biomarkers in Patients with Oral Squamous Cell Carcinoma |
| title_sort | salivary mir 31 5p mir 345 3p and mir 424 3p are reliable biomarkers in patients with oral squamous cell carcinoma |
| topic | oral squamous cell carcinoma saliva miR-345 miR-31 miR-424 real-time qPCR |
| url | https://www.mdpi.com/2076-0817/11/2/229 |
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