Genistein Promotes Anti-Heat Stress and Antioxidant Effects via the Coordinated Regulation of IIS, HSP, MAPK, DR, and Mitochondrial Pathways in <i>Caenorhabditis elegans</i>
To determine the anti-heat stress and antioxidant effects of genistein and the underlying mechanisms, lipofuscin, reactive oxygen species (ROS), and survival under stress were first detected in <i>Caenorhabditis elegans (C. elegans</i>); then the localization and quantification of the fl...
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-01-01
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| Series: | Antioxidants |
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| Online Access: | https://www.mdpi.com/2076-3921/12/1/125 |
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| author | Sai-Ya Zhang Zi-Chen Qin Yi-Yang Sun Yu-Si Chen Wen-Bo Chen Hong-Gang Wang Di An Dan Sun Yan-Qiang Liu |
| author_facet | Sai-Ya Zhang Zi-Chen Qin Yi-Yang Sun Yu-Si Chen Wen-Bo Chen Hong-Gang Wang Di An Dan Sun Yan-Qiang Liu |
| author_sort | Sai-Ya Zhang |
| collection | DOAJ |
| description | To determine the anti-heat stress and antioxidant effects of genistein and the underlying mechanisms, lipofuscin, reactive oxygen species (ROS), and survival under stress were first detected in <i>Caenorhabditis elegans (C. elegans</i>); then the localization and quantification of the fluorescent protein was determined by detecting the fluorescently labeled protein mutant strain; in addition, the aging-related mRNAs were detected by applying real-time fluorescent quantitative PCR in <i>C. elegans</i>. The results indicate that genistein substantially extended the lifespan of <i>C. elegans</i> under oxidative stress and heat conditions; and remarkably reduced the accumulation of lipofuscin in <i>C. elegans</i> under hydrogen peroxide (H<sub>2</sub>O<sub>2</sub>) and 35 °C stress conditions; in addition, it reduced the generation of ROS caused by H<sub>2</sub>O<sub>2</sub> and upregulated the expression of <i>daf-16</i>, <i>ctl-1</i>, <i>hsf-1</i>, <i>hsp-16.2</i>, <i>sip-1</i>, <i>sek-1</i>, <i>pmk-1</i>, and <i>eat-2</i>, whereas it downregulated the expression of <i>age-1</i> and <i>daf-2</i> in <i>C. elegans;</i> similarly, it upregulated the expression of <i>daf-16</i>, <i>sod-3</i>, <i>ctl-1</i>, <i>hsf-1</i>, <i>hsp-16.2</i>, <i>sip-1</i>, <i>sek-1</i>, <i>pmk-1</i>, <i>jnk-1 skn-1</i>, and <i>eat-2</i>, whereas it downregulated the expression of <i>age-1</i>, <i>daf-2</i>, <i>gst-4</i>, and <i>hsp-12.6</i> in <i>C. elegans</i> at 35 °C; moreover, it increased the accumulation of HSP-16.2 and SKN-1 proteins in nematodes under 35 °C and H<sub>2</sub>O<sub>2</sub> conditions; however, it failed to prolong the survival time in the deleted mutant MQ130 nematodes under 35 °C and H<sub>2</sub>O<sub>2</sub> conditions. These results suggest that genistein promote anti-heat stress and antioxidant effects in <i>C. elegans</i> via insulin/-insulin-like growth factor signaling (IIS), heat shock protein (HSP), mitogen-activated protein kinase (MAPK), dietary restriction (DR), and mitochondrial pathways. |
| first_indexed | 2024-03-09T13:46:25Z |
| format | Article |
| id | doaj.art-a8f60aaeb1ca4255889152384469717d |
| institution | Directory Open Access Journal |
| issn | 2076-3921 |
| language | English |
| last_indexed | 2024-03-09T13:46:25Z |
| publishDate | 2023-01-01 |
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| record_format | Article |
| series | Antioxidants |
| spelling | doaj.art-a8f60aaeb1ca4255889152384469717d2023-11-30T20:58:40ZengMDPI AGAntioxidants2076-39212023-01-0112112510.3390/antiox12010125Genistein Promotes Anti-Heat Stress and Antioxidant Effects via the Coordinated Regulation of IIS, HSP, MAPK, DR, and Mitochondrial Pathways in <i>Caenorhabditis elegans</i>Sai-Ya Zhang0Zi-Chen Qin1Yi-Yang Sun2Yu-Si Chen3Wen-Bo Chen4Hong-Gang Wang5Di An6Dan Sun7Yan-Qiang Liu8College of Life Sciences, Nankai University, Tianjin 300071, ChinaCollege of Life Sciences, Nankai University, Tianjin 300071, ChinaCollege of Life Sciences, Nankai University, Tianjin 300071, ChinaCollege of Life Sciences, Nankai University, Tianjin 300071, ChinaCollege of Life Sciences, Nankai University, Tianjin 300071, ChinaCollege of Life Sciences, Nankai University, Tianjin 300071, ChinaCollege of Life Sciences, Nankai University, Tianjin 300071, ChinaCollege of Life Sciences, Nankai University, Tianjin 300071, ChinaCollege of Life Sciences, Nankai University, Tianjin 300071, ChinaTo determine the anti-heat stress and antioxidant effects of genistein and the underlying mechanisms, lipofuscin, reactive oxygen species (ROS), and survival under stress were first detected in <i>Caenorhabditis elegans (C. elegans</i>); then the localization and quantification of the fluorescent protein was determined by detecting the fluorescently labeled protein mutant strain; in addition, the aging-related mRNAs were detected by applying real-time fluorescent quantitative PCR in <i>C. elegans</i>. The results indicate that genistein substantially extended the lifespan of <i>C. elegans</i> under oxidative stress and heat conditions; and remarkably reduced the accumulation of lipofuscin in <i>C. elegans</i> under hydrogen peroxide (H<sub>2</sub>O<sub>2</sub>) and 35 °C stress conditions; in addition, it reduced the generation of ROS caused by H<sub>2</sub>O<sub>2</sub> and upregulated the expression of <i>daf-16</i>, <i>ctl-1</i>, <i>hsf-1</i>, <i>hsp-16.2</i>, <i>sip-1</i>, <i>sek-1</i>, <i>pmk-1</i>, and <i>eat-2</i>, whereas it downregulated the expression of <i>age-1</i> and <i>daf-2</i> in <i>C. elegans;</i> similarly, it upregulated the expression of <i>daf-16</i>, <i>sod-3</i>, <i>ctl-1</i>, <i>hsf-1</i>, <i>hsp-16.2</i>, <i>sip-1</i>, <i>sek-1</i>, <i>pmk-1</i>, <i>jnk-1 skn-1</i>, and <i>eat-2</i>, whereas it downregulated the expression of <i>age-1</i>, <i>daf-2</i>, <i>gst-4</i>, and <i>hsp-12.6</i> in <i>C. elegans</i> at 35 °C; moreover, it increased the accumulation of HSP-16.2 and SKN-1 proteins in nematodes under 35 °C and H<sub>2</sub>O<sub>2</sub> conditions; however, it failed to prolong the survival time in the deleted mutant MQ130 nematodes under 35 °C and H<sub>2</sub>O<sub>2</sub> conditions. These results suggest that genistein promote anti-heat stress and antioxidant effects in <i>C. elegans</i> via insulin/-insulin-like growth factor signaling (IIS), heat shock protein (HSP), mitogen-activated protein kinase (MAPK), dietary restriction (DR), and mitochondrial pathways.https://www.mdpi.com/2076-3921/12/1/125genisteinanti-heat stressantioxidant<i>Caenorhabditis elegans</i> |
| spellingShingle | Sai-Ya Zhang Zi-Chen Qin Yi-Yang Sun Yu-Si Chen Wen-Bo Chen Hong-Gang Wang Di An Dan Sun Yan-Qiang Liu Genistein Promotes Anti-Heat Stress and Antioxidant Effects via the Coordinated Regulation of IIS, HSP, MAPK, DR, and Mitochondrial Pathways in <i>Caenorhabditis elegans</i> Antioxidants genistein anti-heat stress antioxidant <i>Caenorhabditis elegans</i> |
| title | Genistein Promotes Anti-Heat Stress and Antioxidant Effects via the Coordinated Regulation of IIS, HSP, MAPK, DR, and Mitochondrial Pathways in <i>Caenorhabditis elegans</i> |
| title_full | Genistein Promotes Anti-Heat Stress and Antioxidant Effects via the Coordinated Regulation of IIS, HSP, MAPK, DR, and Mitochondrial Pathways in <i>Caenorhabditis elegans</i> |
| title_fullStr | Genistein Promotes Anti-Heat Stress and Antioxidant Effects via the Coordinated Regulation of IIS, HSP, MAPK, DR, and Mitochondrial Pathways in <i>Caenorhabditis elegans</i> |
| title_full_unstemmed | Genistein Promotes Anti-Heat Stress and Antioxidant Effects via the Coordinated Regulation of IIS, HSP, MAPK, DR, and Mitochondrial Pathways in <i>Caenorhabditis elegans</i> |
| title_short | Genistein Promotes Anti-Heat Stress and Antioxidant Effects via the Coordinated Regulation of IIS, HSP, MAPK, DR, and Mitochondrial Pathways in <i>Caenorhabditis elegans</i> |
| title_sort | genistein promotes anti heat stress and antioxidant effects via the coordinated regulation of iis hsp mapk dr and mitochondrial pathways in i caenorhabditis elegans i |
| topic | genistein anti-heat stress antioxidant <i>Caenorhabditis elegans</i> |
| url | https://www.mdpi.com/2076-3921/12/1/125 |
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