Genetic characterization of the human relapsing fever spirochete Borrelia miyamotoi in vectors and animal reservoirs of Lyme disease spirochetes in France

Abstract Background In France as elsewhere in Europe the most prevalent TBD in humans is Lyme borreliosis, caused by different bacterial species belonging to Borrelia burgdorferi sensu lato complex and transmitted by the most important tick species in France, Ixodes ricinus. However, the diagnosis o...

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Main Authors: Jean-François Cosson, Lorraine Michelet, Julien Chotte, Evelyne Le Naour, Martine Cote, Elodie Devillers, Marie-Lazarine Poulle, Dominique Huet, Maxime Galan, Julia Geller, Sara Moutailler, Muriel Vayssier-Taussat
Format: Article
Language:English
Published: BMC 2014-05-01
Series:Parasites & Vectors
Subjects:
Online Access:https://doi.org/10.1186/1756-3305-7-233
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author Jean-François Cosson
Lorraine Michelet
Julien Chotte
Evelyne Le Naour
Martine Cote
Elodie Devillers
Marie-Lazarine Poulle
Dominique Huet
Maxime Galan
Julia Geller
Sara Moutailler
Muriel Vayssier-Taussat
author_facet Jean-François Cosson
Lorraine Michelet
Julien Chotte
Evelyne Le Naour
Martine Cote
Elodie Devillers
Marie-Lazarine Poulle
Dominique Huet
Maxime Galan
Julia Geller
Sara Moutailler
Muriel Vayssier-Taussat
author_sort Jean-François Cosson
collection DOAJ
description Abstract Background In France as elsewhere in Europe the most prevalent TBD in humans is Lyme borreliosis, caused by different bacterial species belonging to Borrelia burgdorferi sensu lato complex and transmitted by the most important tick species in France, Ixodes ricinus. However, the diagnosis of Lyme disease is not always confirmed and unexplained syndromes occurring after tick bites have become an important issue. Recently, B. miyamotoi belonging to the relapsing fever group and transmitted by the same Ixodes species has been involved in human disease in Russia, the USA and the Netherlands. In the present study, we investigate the presence of B. miyamotoi along with other Lyme Borreliosis spirochetes, in ticks and possible animal reservoirs collected in France. Methods We analyzed 268 ticks (Ixodes ricinus) and 72 bank voles (Myodes glareolus) collected and trapped in France for the presence of DNA from B. miyamotoi as well as from Lyme spirochetes using q-PCR and specific primers and probes. We then compared the French genotypes with those found in other European countries. Results We found that 3% of ticks and 5.55% of bank voles were found infected by the same B. miyamotoi genotype, while co-infection with other Lyme spirochetes (B. garinii) was identified in 12% of B. miyamotoi infected ticks. Sequencing showed that ticks and rodents carried the same genotype as those recently characterized in a sick person in the Netherlands. Conclusions The genotype of B. miyamotoi circulating in ticks and bank voles in France is identical to those already described in ticks from Western Europe and to the genotype isolated from a sick person in The Netherlands. This results suggests that even though no human cases have been reported in France, surveillance has to be improved. Moreover, we showed that ticks could simultaneously carry B. miyamotoi and Lyme disease spirochetes, increasing the problem of co-infection in humans.
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spelling doaj.art-a8f85b2a23b44428ba413a1c00dd6c8f2023-06-04T11:15:18ZengBMCParasites & Vectors1756-33052014-05-01711510.1186/1756-3305-7-233Genetic characterization of the human relapsing fever spirochete Borrelia miyamotoi in vectors and animal reservoirs of Lyme disease spirochetes in FranceJean-François Cosson0Lorraine Michelet1Julien Chotte2Evelyne Le Naour3Martine Cote4Elodie Devillers5Marie-Lazarine Poulle6Dominique Huet7Maxime Galan8Julia Geller9Sara Moutailler10Muriel Vayssier-Taussat11INRA, UMR1062, CBGPINRA, USC BiparINRA, USC BiparINRA, USC BiparINRA, USC BiparINRA, USC BiparURCA, SFR Cap Santé, PROTALINRA, USC BiparINRA, UMR1062, CBGPNational Institute for Health DevelopmentINRA, USC BiparINRA, USC BiparAbstract Background In France as elsewhere in Europe the most prevalent TBD in humans is Lyme borreliosis, caused by different bacterial species belonging to Borrelia burgdorferi sensu lato complex and transmitted by the most important tick species in France, Ixodes ricinus. However, the diagnosis of Lyme disease is not always confirmed and unexplained syndromes occurring after tick bites have become an important issue. Recently, B. miyamotoi belonging to the relapsing fever group and transmitted by the same Ixodes species has been involved in human disease in Russia, the USA and the Netherlands. In the present study, we investigate the presence of B. miyamotoi along with other Lyme Borreliosis spirochetes, in ticks and possible animal reservoirs collected in France. Methods We analyzed 268 ticks (Ixodes ricinus) and 72 bank voles (Myodes glareolus) collected and trapped in France for the presence of DNA from B. miyamotoi as well as from Lyme spirochetes using q-PCR and specific primers and probes. We then compared the French genotypes with those found in other European countries. Results We found that 3% of ticks and 5.55% of bank voles were found infected by the same B. miyamotoi genotype, while co-infection with other Lyme spirochetes (B. garinii) was identified in 12% of B. miyamotoi infected ticks. Sequencing showed that ticks and rodents carried the same genotype as those recently characterized in a sick person in the Netherlands. Conclusions The genotype of B. miyamotoi circulating in ticks and bank voles in France is identical to those already described in ticks from Western Europe and to the genotype isolated from a sick person in The Netherlands. This results suggests that even though no human cases have been reported in France, surveillance has to be improved. Moreover, we showed that ticks could simultaneously carry B. miyamotoi and Lyme disease spirochetes, increasing the problem of co-infection in humans.https://doi.org/10.1186/1756-3305-7-233Co-infectionLyme diseaseBank volesTicks
spellingShingle Jean-François Cosson
Lorraine Michelet
Julien Chotte
Evelyne Le Naour
Martine Cote
Elodie Devillers
Marie-Lazarine Poulle
Dominique Huet
Maxime Galan
Julia Geller
Sara Moutailler
Muriel Vayssier-Taussat
Genetic characterization of the human relapsing fever spirochete Borrelia miyamotoi in vectors and animal reservoirs of Lyme disease spirochetes in France
Parasites & Vectors
Co-infection
Lyme disease
Bank voles
Ticks
title Genetic characterization of the human relapsing fever spirochete Borrelia miyamotoi in vectors and animal reservoirs of Lyme disease spirochetes in France
title_full Genetic characterization of the human relapsing fever spirochete Borrelia miyamotoi in vectors and animal reservoirs of Lyme disease spirochetes in France
title_fullStr Genetic characterization of the human relapsing fever spirochete Borrelia miyamotoi in vectors and animal reservoirs of Lyme disease spirochetes in France
title_full_unstemmed Genetic characterization of the human relapsing fever spirochete Borrelia miyamotoi in vectors and animal reservoirs of Lyme disease spirochetes in France
title_short Genetic characterization of the human relapsing fever spirochete Borrelia miyamotoi in vectors and animal reservoirs of Lyme disease spirochetes in France
title_sort genetic characterization of the human relapsing fever spirochete borrelia miyamotoi in vectors and animal reservoirs of lyme disease spirochetes in france
topic Co-infection
Lyme disease
Bank voles
Ticks
url https://doi.org/10.1186/1756-3305-7-233
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