Akkermansia muciniphila-Derived Extracellular Vesicles as a Mucosal Delivery Vector for Amelioration of Obesity in Mice
Recent evidence suggests that probiotics can restore the mucosal barrier integrity, ameliorate inflammation, and promote homeostasis required for metabolism in obesity by affecting the gut microbiota composition. In this study, we investigated the effect of Akkermansia muciniphila and its extracellu...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2019-10-01
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| Series: | Frontiers in Microbiology |
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| Online Access: | https://www.frontiersin.org/article/10.3389/fmicb.2019.02155/full |
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| author | Fatemeh Ashrafian Fatemeh Ashrafian Arefeh Shahriary Arefeh Shahriary Ava Behrouzi Ava Behrouzi Hamid Reza Moradi Shahrbanoo Keshavarz Azizi Raftar Shahrbanoo Keshavarz Azizi Raftar Arezou Lari Shima Hadifar Shima Hadifar Rezvan Yaghoubfar Rezvan Yaghoubfar Sara Ahmadi Badi Sara Ahmadi Badi Shohre Khatami Farzam Vaziri Farzam Vaziri Seyed Davar Siadat Seyed Davar Siadat |
| author_facet | Fatemeh Ashrafian Fatemeh Ashrafian Arefeh Shahriary Arefeh Shahriary Ava Behrouzi Ava Behrouzi Hamid Reza Moradi Shahrbanoo Keshavarz Azizi Raftar Shahrbanoo Keshavarz Azizi Raftar Arezou Lari Shima Hadifar Shima Hadifar Rezvan Yaghoubfar Rezvan Yaghoubfar Sara Ahmadi Badi Sara Ahmadi Badi Shohre Khatami Farzam Vaziri Farzam Vaziri Seyed Davar Siadat Seyed Davar Siadat |
| author_sort | Fatemeh Ashrafian |
| collection | DOAJ |
| description | Recent evidence suggests that probiotics can restore the mucosal barrier integrity, ameliorate inflammation, and promote homeostasis required for metabolism in obesity by affecting the gut microbiota composition. In this study, we investigated the effect of Akkermansia muciniphila and its extracellular vesicles (EVs) on obesity-related genes in microarray datasets and evaluated the cell line and C57BL/6 mice by conducting RT-PCR and ELISA assays. A. muciniphila-derived EVs caused a more significant loss in body and fat weight of high-fat diet (HFD)-fed mice, compared with the bacterium itself. Moreover, treatment with A. muciniphila and EVs had significant effects on lipid metabolism and expression of inflammatory markers in adipose tissues. Both treatments improved the intestinal barrier integrity, inflammation, energy balance, and blood parameters (i.e., lipid profile and glucose level). Our findings showed that A. muciniphila-derived EVs contain various biomolecules, which can have a positive impact on obesity by affecting the involved genes. Also, our results showed that A. muciniphila and its EVs had a significant relationship with intestinal homeostasis, which highlights their positive role in obesity treatment. In conclusion, A. muciniphila-derived EVs can be used as new therapeutic strategies to ameliorate HFD-induced obesity by affecting various mechanisms. |
| first_indexed | 2024-12-10T06:25:56Z |
| format | Article |
| id | doaj.art-a8f8e768ece94e99b107fdcc3f69e071 |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-12-10T06:25:56Z |
| publishDate | 2019-10-01 |
| publisher | Frontiers Media S.A. |
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| series | Frontiers in Microbiology |
| spelling | doaj.art-a8f8e768ece94e99b107fdcc3f69e0712022-12-22T01:59:12ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2019-10-011010.3389/fmicb.2019.02155483128Akkermansia muciniphila-Derived Extracellular Vesicles as a Mucosal Delivery Vector for Amelioration of Obesity in MiceFatemeh Ashrafian0Fatemeh Ashrafian1Arefeh Shahriary2Arefeh Shahriary3Ava Behrouzi4Ava Behrouzi5Hamid Reza Moradi6Shahrbanoo Keshavarz Azizi Raftar7Shahrbanoo Keshavarz Azizi Raftar8Arezou Lari9Shima Hadifar10Shima Hadifar11Rezvan Yaghoubfar12Rezvan Yaghoubfar13Sara Ahmadi Badi14Sara Ahmadi Badi15Shohre Khatami16Farzam Vaziri17Farzam Vaziri18Seyed Davar Siadat19Seyed Davar Siadat20Department of Mycobacteriology and Pulmonary Research, Pasteur Institute of Iran, Tehran, IranMicrobiology Research Center, Pasteur Institute of Iran, Tehran, IranDepartment of Mycobacteriology and Pulmonary Research, Pasteur Institute of Iran, Tehran, IranMicrobiology Research Center, Pasteur Institute of Iran, Tehran, IranDepartment of Mycobacteriology and Pulmonary Research, Pasteur Institute of Iran, Tehran, IranMicrobiology Research Center, Pasteur Institute of Iran, Tehran, IranDepartment of Histology and Embryology Group, Basic Sciences, Faculty of Veterinary Medicine, Shiraz University, Shiraz, IranDepartment of Mycobacteriology and Pulmonary Research, Pasteur Institute of Iran, Tehran, IranMicrobiology Research Center, Pasteur Institute of Iran, Tehran, IranSystems Biomedicine Unit, Pasteur Institute of Iran, Tehran, IranDepartment of Mycobacteriology and Pulmonary Research, Pasteur Institute of Iran, Tehran, IranMicrobiology Research Center, Pasteur Institute of Iran, Tehran, IranDepartment of Mycobacteriology and Pulmonary Research, Pasteur Institute of Iran, Tehran, IranMicrobiology Research Center, Pasteur Institute of Iran, Tehran, IranDepartment of Mycobacteriology and Pulmonary Research, Pasteur Institute of Iran, Tehran, IranMicrobiology Research Center, Pasteur Institute of Iran, Tehran, IranDepartment of Biochemistry, Pasteur Institute of Iran, Tehran, IranDepartment of Mycobacteriology and Pulmonary Research, Pasteur Institute of Iran, Tehran, IranMicrobiology Research Center, Pasteur Institute of Iran, Tehran, IranDepartment of Mycobacteriology and Pulmonary Research, Pasteur Institute of Iran, Tehran, IranMicrobiology Research Center, Pasteur Institute of Iran, Tehran, IranRecent evidence suggests that probiotics can restore the mucosal barrier integrity, ameliorate inflammation, and promote homeostasis required for metabolism in obesity by affecting the gut microbiota composition. In this study, we investigated the effect of Akkermansia muciniphila and its extracellular vesicles (EVs) on obesity-related genes in microarray datasets and evaluated the cell line and C57BL/6 mice by conducting RT-PCR and ELISA assays. A. muciniphila-derived EVs caused a more significant loss in body and fat weight of high-fat diet (HFD)-fed mice, compared with the bacterium itself. Moreover, treatment with A. muciniphila and EVs had significant effects on lipid metabolism and expression of inflammatory markers in adipose tissues. Both treatments improved the intestinal barrier integrity, inflammation, energy balance, and blood parameters (i.e., lipid profile and glucose level). Our findings showed that A. muciniphila-derived EVs contain various biomolecules, which can have a positive impact on obesity by affecting the involved genes. Also, our results showed that A. muciniphila and its EVs had a significant relationship with intestinal homeostasis, which highlights their positive role in obesity treatment. In conclusion, A. muciniphila-derived EVs can be used as new therapeutic strategies to ameliorate HFD-induced obesity by affecting various mechanisms.https://www.frontiersin.org/article/10.3389/fmicb.2019.02155/fullgut microbiotaAkkermansia muciniphilaextracellular vesiclestoll-like receptorstight junctionperoxisome proliferator-activated receptors |
| spellingShingle | Fatemeh Ashrafian Fatemeh Ashrafian Arefeh Shahriary Arefeh Shahriary Ava Behrouzi Ava Behrouzi Hamid Reza Moradi Shahrbanoo Keshavarz Azizi Raftar Shahrbanoo Keshavarz Azizi Raftar Arezou Lari Shima Hadifar Shima Hadifar Rezvan Yaghoubfar Rezvan Yaghoubfar Sara Ahmadi Badi Sara Ahmadi Badi Shohre Khatami Farzam Vaziri Farzam Vaziri Seyed Davar Siadat Seyed Davar Siadat Akkermansia muciniphila-Derived Extracellular Vesicles as a Mucosal Delivery Vector for Amelioration of Obesity in Mice Frontiers in Microbiology gut microbiota Akkermansia muciniphila extracellular vesicles toll-like receptors tight junction peroxisome proliferator-activated receptors |
| title | Akkermansia muciniphila-Derived Extracellular Vesicles as a Mucosal Delivery Vector for Amelioration of Obesity in Mice |
| title_full | Akkermansia muciniphila-Derived Extracellular Vesicles as a Mucosal Delivery Vector for Amelioration of Obesity in Mice |
| title_fullStr | Akkermansia muciniphila-Derived Extracellular Vesicles as a Mucosal Delivery Vector for Amelioration of Obesity in Mice |
| title_full_unstemmed | Akkermansia muciniphila-Derived Extracellular Vesicles as a Mucosal Delivery Vector for Amelioration of Obesity in Mice |
| title_short | Akkermansia muciniphila-Derived Extracellular Vesicles as a Mucosal Delivery Vector for Amelioration of Obesity in Mice |
| title_sort | akkermansia muciniphila derived extracellular vesicles as a mucosal delivery vector for amelioration of obesity in mice |
| topic | gut microbiota Akkermansia muciniphila extracellular vesicles toll-like receptors tight junction peroxisome proliferator-activated receptors |
| url | https://www.frontiersin.org/article/10.3389/fmicb.2019.02155/full |
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