Three-Dimensional Porous Scaffolds Derived from Bovine Cancellous Bone Matrix Promote Osteoinduction, Osteoconduction, and Osteogenesis
The use of three-dimensional porous scaffolds derived from decellularized extracellular matrix (ECM) is increasing for functional repair and regeneration of injured bone tissue. Because these scaffolds retain their native structures and bioactive molecules, in addition to showing low immunogenicity...
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| Format: | Article |
| Language: | English |
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MDPI AG
2021-12-01
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| Series: | Polymers |
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| Online Access: | https://www.mdpi.com/2073-4360/13/24/4390 |
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| author | Alda Malagón-Escandón Mathieu Hautefeuille Edgar Jimenez-Díaz Jesus Arenas-Alatorre José Manuel Saniger Isidro Badillo-Ramírez Nadia Vazquez Gabriela Piñón-Zarate Andrés Castell-Rodríguez |
| author_facet | Alda Malagón-Escandón Mathieu Hautefeuille Edgar Jimenez-Díaz Jesus Arenas-Alatorre José Manuel Saniger Isidro Badillo-Ramírez Nadia Vazquez Gabriela Piñón-Zarate Andrés Castell-Rodríguez |
| author_sort | Alda Malagón-Escandón |
| collection | DOAJ |
| description | The use of three-dimensional porous scaffolds derived from decellularized extracellular matrix (ECM) is increasing for functional repair and regeneration of injured bone tissue. Because these scaffolds retain their native structures and bioactive molecules, in addition to showing low immunogenicity and good biodegradability, they can promote tissue repair and regeneration. Nonetheless, imitating these features in synthetic materials represents a challenging task. Furthermore, due to the complexity of bone tissue, different processes are necessary to maintain these characteristics. We present a novel approach using decellularized ECM material derived from bovine cancellous bone by demineralization, decellularization, and hydrolysis of collagen to obtain a three-dimensional porous scaffold. This study demonstrates that the three-dimensional porous scaffold obtained from bovine bone retained its osteoconductive and osteoinductive properties and presented osteogenic potential when seeded with human Wharton’s jelly mesenchymal stromal cells (hWJ-MSCs). Based on its characteristics, the scaffold described in this work potentially represents a therapeutic strategy for bone repair. |
| first_indexed | 2024-03-10T03:15:48Z |
| format | Article |
| id | doaj.art-a8fa88ba336e4f7aa4da87b84ae8f067 |
| institution | Directory Open Access Journal |
| issn | 2073-4360 |
| language | English |
| last_indexed | 2024-03-10T03:15:48Z |
| publishDate | 2021-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Polymers |
| spelling | doaj.art-a8fa88ba336e4f7aa4da87b84ae8f0672023-11-23T10:15:41ZengMDPI AGPolymers2073-43602021-12-011324439010.3390/polym13244390Three-Dimensional Porous Scaffolds Derived from Bovine Cancellous Bone Matrix Promote Osteoinduction, Osteoconduction, and OsteogenesisAlda Malagón-Escandón0Mathieu Hautefeuille1Edgar Jimenez-Díaz2Jesus Arenas-Alatorre3José Manuel Saniger4Isidro Badillo-Ramírez5Nadia Vazquez6Gabriela Piñón-Zarate7Andrés Castell-Rodríguez8Facultad de Medicina, UNAM, Mexico City C.P. 04510, MexicoFacultad de Ciencias, UNAM, Mexico City C.P. 04510, MexicoFacultad de Ciencias, UNAM, Mexico City C.P. 04510, MexicoInstituto de Física, UNAM, Mexico City C.P. 04510, MexicoInstituto de Ciencias Aplicadas y Tecnología (ICAT), UNAM, Mexico City C.P. 04510, MexicoInstituto de Ciencias Aplicadas y Tecnología (ICAT), UNAM, Mexico City C.P. 04510, MexicoFacultad de Medicina, UNAM, Mexico City C.P. 04510, MexicoFacultad de Medicina, UNAM, Mexico City C.P. 04510, MexicoFacultad de Medicina, UNAM, Mexico City C.P. 04510, MexicoThe use of three-dimensional porous scaffolds derived from decellularized extracellular matrix (ECM) is increasing for functional repair and regeneration of injured bone tissue. Because these scaffolds retain their native structures and bioactive molecules, in addition to showing low immunogenicity and good biodegradability, they can promote tissue repair and regeneration. Nonetheless, imitating these features in synthetic materials represents a challenging task. Furthermore, due to the complexity of bone tissue, different processes are necessary to maintain these characteristics. We present a novel approach using decellularized ECM material derived from bovine cancellous bone by demineralization, decellularization, and hydrolysis of collagen to obtain a three-dimensional porous scaffold. This study demonstrates that the three-dimensional porous scaffold obtained from bovine bone retained its osteoconductive and osteoinductive properties and presented osteogenic potential when seeded with human Wharton’s jelly mesenchymal stromal cells (hWJ-MSCs). Based on its characteristics, the scaffold described in this work potentially represents a therapeutic strategy for bone repair.https://www.mdpi.com/2073-4360/13/24/4390three-dimensional porous scaffoldsbone extracellular matrixbone regenerationtissue engineeringbiomaterials |
| spellingShingle | Alda Malagón-Escandón Mathieu Hautefeuille Edgar Jimenez-Díaz Jesus Arenas-Alatorre José Manuel Saniger Isidro Badillo-Ramírez Nadia Vazquez Gabriela Piñón-Zarate Andrés Castell-Rodríguez Three-Dimensional Porous Scaffolds Derived from Bovine Cancellous Bone Matrix Promote Osteoinduction, Osteoconduction, and Osteogenesis Polymers three-dimensional porous scaffolds bone extracellular matrix bone regeneration tissue engineering biomaterials |
| title | Three-Dimensional Porous Scaffolds Derived from Bovine Cancellous Bone Matrix Promote Osteoinduction, Osteoconduction, and Osteogenesis |
| title_full | Three-Dimensional Porous Scaffolds Derived from Bovine Cancellous Bone Matrix Promote Osteoinduction, Osteoconduction, and Osteogenesis |
| title_fullStr | Three-Dimensional Porous Scaffolds Derived from Bovine Cancellous Bone Matrix Promote Osteoinduction, Osteoconduction, and Osteogenesis |
| title_full_unstemmed | Three-Dimensional Porous Scaffolds Derived from Bovine Cancellous Bone Matrix Promote Osteoinduction, Osteoconduction, and Osteogenesis |
| title_short | Three-Dimensional Porous Scaffolds Derived from Bovine Cancellous Bone Matrix Promote Osteoinduction, Osteoconduction, and Osteogenesis |
| title_sort | three dimensional porous scaffolds derived from bovine cancellous bone matrix promote osteoinduction osteoconduction and osteogenesis |
| topic | three-dimensional porous scaffolds bone extracellular matrix bone regeneration tissue engineering biomaterials |
| url | https://www.mdpi.com/2073-4360/13/24/4390 |
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