Modulation of Type I Interferon System by African Swine Fever Virus
African Swine Fever Virus (ASFV) has tropism for macrophages, which seems to play a crucial role in disease pathogenesis and viral dissemination. Previous studies showed that ASFV developed mechanisms to evade type I interferon (IFN) responses. Hence, we analyzed the ability of ASFV strains of diver...
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MDPI AG
2020-05-01
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| Series: | Pathogens |
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| Online Access: | https://www.mdpi.com/2076-0817/9/5/361 |
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| author | Elisabetta Razzuoli Giulia Franzoni Tania Carta Susanna Zinellu Massimo Amadori Paola Modesto Annalisa Oggiano |
| author_facet | Elisabetta Razzuoli Giulia Franzoni Tania Carta Susanna Zinellu Massimo Amadori Paola Modesto Annalisa Oggiano |
| author_sort | Elisabetta Razzuoli |
| collection | DOAJ |
| description | African Swine Fever Virus (ASFV) has tropism for macrophages, which seems to play a crucial role in disease pathogenesis and viral dissemination. Previous studies showed that ASFV developed mechanisms to evade type I interferon (IFN) responses. Hence, we analyzed the ability of ASFV strains of diverse virulence to modulate IFN-β and IFN-α responses. Porcine monocyte-derived macrophages un-activated (moMΦ) or activated with IFN-α (moMΦ + FN-α) were infected with virulent (22653/14) or attenuated (NH/P68) ASFV strains, and expressions of IFN-β and of 17 IFN-α subtypes genes were monitored over time. ASFV strains of diverse virulence induced different panels of IFN genes: infection of moMΦ with either strains caused statistically significant up-regulation of IFN-α3, -α7/11, whereas only attenuated NH/P68 determined statistically significant up-regulation of IFN-α10, -α12, -α13, -α15, -α17, and IFN-β. Infection of activated moMΦ with either strains resulted in up-regulation of IFN-β and many IFN-α subtypes, but statistical significance was found only for IFN-α1, -α10, -α15, -α16, -α17 in response to NH/P68-infection only. These data revealed differences in type I IFNs expression patterns, with differences between strains of diverse virulence. In addition, virulent 22653/14 ASFV seems to have developed mechanisms to suppress the induction of several type I IFN genes. |
| first_indexed | 2024-03-10T19:56:41Z |
| format | Article |
| id | doaj.art-a8fbb6823f97455eac83bd9ebd5e8863 |
| institution | Directory Open Access Journal |
| issn | 2076-0817 |
| language | English |
| last_indexed | 2024-03-10T19:56:41Z |
| publishDate | 2020-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Pathogens |
| spelling | doaj.art-a8fbb6823f97455eac83bd9ebd5e88632023-11-19T23:53:02ZengMDPI AGPathogens2076-08172020-05-019536110.3390/pathogens9050361Modulation of Type I Interferon System by African Swine Fever VirusElisabetta Razzuoli0Giulia Franzoni1Tania Carta2Susanna Zinellu3Massimo Amadori4Paola Modesto5Annalisa Oggiano6Department of Genoa, Istituto Zooprofilattico Sperimentale del Piemonte, Liguria e Valle D’Aosta, 16129 Genova, ItalyDepartment of Animal Health, Istituto Zooprofilattico Sperimentale della Sardegna, 07100 Sassari, ItalyDepartment of Animal Health, Istituto Zooprofilattico Sperimentale della Sardegna, 07100 Sassari, ItalyDepartment of Animal Health, Istituto Zooprofilattico Sperimentale della Sardegna, 07100 Sassari, ItalyLaboratory of Cellular Immunology, Istituto Zooprofilattico della Lombardia e dell’Emilia Romagna (IZSLER), 25124 Brescia, ItalyDepartment of Genoa, Istituto Zooprofilattico Sperimentale del Piemonte, Liguria e Valle D’Aosta, 16129 Genova, ItalyDepartment of Animal Health, Istituto Zooprofilattico Sperimentale della Sardegna, 07100 Sassari, ItalyAfrican Swine Fever Virus (ASFV) has tropism for macrophages, which seems to play a crucial role in disease pathogenesis and viral dissemination. Previous studies showed that ASFV developed mechanisms to evade type I interferon (IFN) responses. Hence, we analyzed the ability of ASFV strains of diverse virulence to modulate IFN-β and IFN-α responses. Porcine monocyte-derived macrophages un-activated (moMΦ) or activated with IFN-α (moMΦ + FN-α) were infected with virulent (22653/14) or attenuated (NH/P68) ASFV strains, and expressions of IFN-β and of 17 IFN-α subtypes genes were monitored over time. ASFV strains of diverse virulence induced different panels of IFN genes: infection of moMΦ with either strains caused statistically significant up-regulation of IFN-α3, -α7/11, whereas only attenuated NH/P68 determined statistically significant up-regulation of IFN-α10, -α12, -α13, -α15, -α17, and IFN-β. Infection of activated moMΦ with either strains resulted in up-regulation of IFN-β and many IFN-α subtypes, but statistical significance was found only for IFN-α1, -α10, -α15, -α16, -α17 in response to NH/P68-infection only. These data revealed differences in type I IFNs expression patterns, with differences between strains of diverse virulence. In addition, virulent 22653/14 ASFV seems to have developed mechanisms to suppress the induction of several type I IFN genes.https://www.mdpi.com/2076-0817/9/5/361African swine feverIFN-α subtypesIFN-βmonocyte-derived macrophagesINF-α activation |
| spellingShingle | Elisabetta Razzuoli Giulia Franzoni Tania Carta Susanna Zinellu Massimo Amadori Paola Modesto Annalisa Oggiano Modulation of Type I Interferon System by African Swine Fever Virus Pathogens African swine fever IFN-α subtypes IFN-β monocyte-derived macrophages INF-α activation |
| title | Modulation of Type I Interferon System by African Swine Fever Virus |
| title_full | Modulation of Type I Interferon System by African Swine Fever Virus |
| title_fullStr | Modulation of Type I Interferon System by African Swine Fever Virus |
| title_full_unstemmed | Modulation of Type I Interferon System by African Swine Fever Virus |
| title_short | Modulation of Type I Interferon System by African Swine Fever Virus |
| title_sort | modulation of type i interferon system by african swine fever virus |
| topic | African swine fever IFN-α subtypes IFN-β monocyte-derived macrophages INF-α activation |
| url | https://www.mdpi.com/2076-0817/9/5/361 |
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