In-frame cDNA library combined with protein complementation assay identifies ARL11-binding partners.
The cDNA expression libraries that produce correct proteins are essential in facilitating the identification of protein-protein interactions. The 5'-untranslated regions (UTRs) that are present in the majority of mammalian and non-mammalian genes are predicted to alter the expression of correct...
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2012-01-01
|
| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC3525598?pdf=render |
| _version_ | 1818500183137189888 |
|---|---|
| author | Sangkyou Lee Ilkyun Lee Yoonsuh Jung David McConkey Bogdan Czerniak |
| author_facet | Sangkyou Lee Ilkyun Lee Yoonsuh Jung David McConkey Bogdan Czerniak |
| author_sort | Sangkyou Lee |
| collection | DOAJ |
| description | The cDNA expression libraries that produce correct proteins are essential in facilitating the identification of protein-protein interactions. The 5'-untranslated regions (UTRs) that are present in the majority of mammalian and non-mammalian genes are predicted to alter the expression of correct proteins from cDNA libraries. We developed a novel cDNA expression library from which 5'-UTRs were removed using a mixture of polymerase chain reaction primers that complement the Kozak sequences we refer to as an "in-frame cDNA library." We used this library with the protein complementation assay to identify two novel binding partners for ras-related ADP-ribosylation factor-like 11 (ARL11), cellular retinoic acid binding protein 2 (CRABP2), and phosphoglycerate mutase 1 (PGAM1). Thus, the in-frame cDNA library without 5'-UTRs we describe here increases the chance of correctly identifying protein interactions and will have wide applications in both mammalian and non-mammalian detection systems. |
| first_indexed | 2024-12-10T20:39:23Z |
| format | Article |
| id | doaj.art-a8fe1f20778c4e088de8fb223ff334ce |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-10T20:39:23Z |
| publishDate | 2012-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-a8fe1f20778c4e088de8fb223ff334ce2022-12-22T01:34:25ZengPublic Library of Science (PLoS)PLoS ONE1932-62032012-01-01712e5229010.1371/journal.pone.0052290In-frame cDNA library combined with protein complementation assay identifies ARL11-binding partners.Sangkyou LeeIlkyun LeeYoonsuh JungDavid McConkeyBogdan CzerniakThe cDNA expression libraries that produce correct proteins are essential in facilitating the identification of protein-protein interactions. The 5'-untranslated regions (UTRs) that are present in the majority of mammalian and non-mammalian genes are predicted to alter the expression of correct proteins from cDNA libraries. We developed a novel cDNA expression library from which 5'-UTRs were removed using a mixture of polymerase chain reaction primers that complement the Kozak sequences we refer to as an "in-frame cDNA library." We used this library with the protein complementation assay to identify two novel binding partners for ras-related ADP-ribosylation factor-like 11 (ARL11), cellular retinoic acid binding protein 2 (CRABP2), and phosphoglycerate mutase 1 (PGAM1). Thus, the in-frame cDNA library without 5'-UTRs we describe here increases the chance of correctly identifying protein interactions and will have wide applications in both mammalian and non-mammalian detection systems.http://europepmc.org/articles/PMC3525598?pdf=render |
| spellingShingle | Sangkyou Lee Ilkyun Lee Yoonsuh Jung David McConkey Bogdan Czerniak In-frame cDNA library combined with protein complementation assay identifies ARL11-binding partners. PLoS ONE |
| title | In-frame cDNA library combined with protein complementation assay identifies ARL11-binding partners. |
| title_full | In-frame cDNA library combined with protein complementation assay identifies ARL11-binding partners. |
| title_fullStr | In-frame cDNA library combined with protein complementation assay identifies ARL11-binding partners. |
| title_full_unstemmed | In-frame cDNA library combined with protein complementation assay identifies ARL11-binding partners. |
| title_short | In-frame cDNA library combined with protein complementation assay identifies ARL11-binding partners. |
| title_sort | in frame cdna library combined with protein complementation assay identifies arl11 binding partners |
| url | http://europepmc.org/articles/PMC3525598?pdf=render |
| work_keys_str_mv | AT sangkyoulee inframecdnalibrarycombinedwithproteincomplementationassayidentifiesarl11bindingpartners AT ilkyunlee inframecdnalibrarycombinedwithproteincomplementationassayidentifiesarl11bindingpartners AT yoonsuhjung inframecdnalibrarycombinedwithproteincomplementationassayidentifiesarl11bindingpartners AT davidmcconkey inframecdnalibrarycombinedwithproteincomplementationassayidentifiesarl11bindingpartners AT bogdanczerniak inframecdnalibrarycombinedwithproteincomplementationassayidentifiesarl11bindingpartners |