Single-Molecular Förster Resonance Energy Transfer Measurement on Structures and Interactions of Biomolecules
Single-molecule Förster resonance energy transfer (smFRET) inherits the strategy of measurement from the effective “spectroscopic ruler” FRET and can be utilized to observe molecular behaviors with relatively high throughput at nanometer scale. The simplicity in principle and configuration of smFRET...
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| Format: | Article |
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MDPI AG
2021-04-01
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| Series: | Micromachines |
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| Online Access: | https://www.mdpi.com/2072-666X/12/5/492 |
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| author | Yi Qiao Yuhan Luo Naiyun Long Yi Xing Jing Tu |
| author_facet | Yi Qiao Yuhan Luo Naiyun Long Yi Xing Jing Tu |
| author_sort | Yi Qiao |
| collection | DOAJ |
| description | Single-molecule Förster resonance energy transfer (smFRET) inherits the strategy of measurement from the effective “spectroscopic ruler” FRET and can be utilized to observe molecular behaviors with relatively high throughput at nanometer scale. The simplicity in principle and configuration of smFRET make it easy to apply and couple with other technologies to comprehensively understand single-molecule dynamics in various application scenarios. Despite its widespread application, smFRET is continuously developing and novel studies based on the advanced platforms have been done. Here, we summarize some representative examples of smFRET research of recent years to exhibit the versatility and note typical strategies to further improve the performance of smFRET measurement on different biomolecules. |
| first_indexed | 2024-03-10T11:56:11Z |
| format | Article |
| id | doaj.art-a8ff047137f642c6b823f1564c48bfe2 |
| institution | Directory Open Access Journal |
| issn | 2072-666X |
| language | English |
| last_indexed | 2024-03-10T11:56:11Z |
| publishDate | 2021-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Micromachines |
| spelling | doaj.art-a8ff047137f642c6b823f1564c48bfe22023-11-21T17:18:54ZengMDPI AGMicromachines2072-666X2021-04-0112549210.3390/mi12050492Single-Molecular Förster Resonance Energy Transfer Measurement on Structures and Interactions of BiomoleculesYi Qiao0Yuhan Luo1Naiyun Long2Yi Xing3Jing Tu4State Key Laboratory of Bioelectronics, School of Biological Science and Medical Engineering, Southeast University, Nanjing 210096, ChinaState Key Laboratory of Bioelectronics, School of Biological Science and Medical Engineering, Southeast University, Nanjing 210096, ChinaState Key Laboratory of Bioelectronics, School of Biological Science and Medical Engineering, Southeast University, Nanjing 210096, ChinaInstitute of Child and Adolescent Health, School of Public Health, Peking University, Beijing 100191, ChinaState Key Laboratory of Bioelectronics, School of Biological Science and Medical Engineering, Southeast University, Nanjing 210096, ChinaSingle-molecule Förster resonance energy transfer (smFRET) inherits the strategy of measurement from the effective “spectroscopic ruler” FRET and can be utilized to observe molecular behaviors with relatively high throughput at nanometer scale. The simplicity in principle and configuration of smFRET make it easy to apply and couple with other technologies to comprehensively understand single-molecule dynamics in various application scenarios. Despite its widespread application, smFRET is continuously developing and novel studies based on the advanced platforms have been done. Here, we summarize some representative examples of smFRET research of recent years to exhibit the versatility and note typical strategies to further improve the performance of smFRET measurement on different biomolecules.https://www.mdpi.com/2072-666X/12/5/492FRETsmFRETbiomoleculenucleic acid structureprotein |
| spellingShingle | Yi Qiao Yuhan Luo Naiyun Long Yi Xing Jing Tu Single-Molecular Förster Resonance Energy Transfer Measurement on Structures and Interactions of Biomolecules Micromachines FRET smFRET biomolecule nucleic acid structure protein |
| title | Single-Molecular Förster Resonance Energy Transfer Measurement on Structures and Interactions of Biomolecules |
| title_full | Single-Molecular Förster Resonance Energy Transfer Measurement on Structures and Interactions of Biomolecules |
| title_fullStr | Single-Molecular Förster Resonance Energy Transfer Measurement on Structures and Interactions of Biomolecules |
| title_full_unstemmed | Single-Molecular Förster Resonance Energy Transfer Measurement on Structures and Interactions of Biomolecules |
| title_short | Single-Molecular Förster Resonance Energy Transfer Measurement on Structures and Interactions of Biomolecules |
| title_sort | single molecular forster resonance energy transfer measurement on structures and interactions of biomolecules |
| topic | FRET smFRET biomolecule nucleic acid structure protein |
| url | https://www.mdpi.com/2072-666X/12/5/492 |
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