Transcription factor 3 (TCF3) combined with histone deacetylase 3 (HDAC3) down-regulates microRNA-101 to promote Burkitt lymphoma cell proliferation and inhibit apoptosis
To explore the function of transcription factor 3 (TCF3) on the proliferation and apoptosis of Burkitt lymphoma cells and its mechanism. qRT-PCR was performed to determine the expression of TCF3, histone deacetylase 3 (HDAC3), and microRNA-101 (miR-101) in the Burkitt lymphoma (BL) tumor tissues and...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2021-01-01
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| Series: | Bioengineered |
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| Online Access: | http://dx.doi.org/10.1080/21655979.2021.1977557 |
| _version_ | 1798033994264084480 |
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| author | Lihua Dong Jingjing Huang Peng Zu Jing Liu Xue Gao Jianwei Du Yufu Li |
| author_facet | Lihua Dong Jingjing Huang Peng Zu Jing Liu Xue Gao Jianwei Du Yufu Li |
| author_sort | Lihua Dong |
| collection | DOAJ |
| description | To explore the function of transcription factor 3 (TCF3) on the proliferation and apoptosis of Burkitt lymphoma cells and its mechanism. qRT-PCR was performed to determine the expression of TCF3, histone deacetylase 3 (HDAC3), and microRNA-101 (miR-101) in the Burkitt lymphoma (BL) tumor tissues and lymph node tissues with reactive lymph node hyperplasia (RLNH). We found that the expression of TCF3 and HDAC3 was up-regulated in BL tumor tissues and lymphoma cells, and the miR-101 expression was down-regulated. And TCF3 and HDAC3 were negatively correlated with the expression of miR-101, respectively. In addition, knockdown of TCF3 can inhibit BL cell proliferation, reduce cell viability and promote cell apoptosis, retain the cell cycle in the G0/G1 phase, and inhibit the expression of Akt/mTOR pathway-related proteins (p-Akt and p-mTOR). When miR-101 was overexpressed, the results were the same as when TCF3 was knocked down. Moreover, we used Co-immunoprecipitation (Co-IP) to detect the interaction between TCF3 and HDAC3, and performed the Chromatin immunoprecipitation (ChIP) experiment to detect the enrichment of TCF3 and HDAC3 in the promoter region of miR-101. We found that TCF3 can interact with HDAC3 and is enriched in the miR-101 promoter region. In conclusion, TCF3 combined with HDAC3 down-regulates the expression of miR-101, thereby promoting the proliferation of BL cells and inhibiting their apoptosis. |
| first_indexed | 2024-04-11T20:38:06Z |
| format | Article |
| id | doaj.art-a900043f2353427cb3affe2db540c2ab |
| institution | Directory Open Access Journal |
| issn | 2165-5979 2165-5987 |
| language | English |
| last_indexed | 2024-04-11T20:38:06Z |
| publishDate | 2021-01-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Bioengineered |
| spelling | doaj.art-a900043f2353427cb3affe2db540c2ab2022-12-22T04:04:18ZengTaylor & Francis GroupBioengineered2165-59792165-59872021-01-011217995800510.1080/21655979.2021.19775571977557Transcription factor 3 (TCF3) combined with histone deacetylase 3 (HDAC3) down-regulates microRNA-101 to promote Burkitt lymphoma cell proliferation and inhibit apoptosisLihua Dong0Jingjing Huang1Peng Zu2Jing Liu3Xue Gao4Jianwei Du5Yufu Li6Henan Institute of Hematology, the Affiliated Cancer Hospital of Zhengzhou UniversityHenan Institute of Hematology, the Affiliated Cancer Hospital of Zhengzhou UniversityHenan Institute of Hematology, the Affiliated Cancer Hospital of Zhengzhou UniversityHenan Institute of Hematology, the Affiliated Cancer Hospital of Zhengzhou UniversityHenan Institute of Hematology, the Affiliated Cancer Hospital of Zhengzhou UniversityHenan Institute of Hematology, the Affiliated Cancer Hospital of Zhengzhou UniversityHenan Institute of Hematology, the Affiliated Cancer Hospital of Zhengzhou UniversityTo explore the function of transcription factor 3 (TCF3) on the proliferation and apoptosis of Burkitt lymphoma cells and its mechanism. qRT-PCR was performed to determine the expression of TCF3, histone deacetylase 3 (HDAC3), and microRNA-101 (miR-101) in the Burkitt lymphoma (BL) tumor tissues and lymph node tissues with reactive lymph node hyperplasia (RLNH). We found that the expression of TCF3 and HDAC3 was up-regulated in BL tumor tissues and lymphoma cells, and the miR-101 expression was down-regulated. And TCF3 and HDAC3 were negatively correlated with the expression of miR-101, respectively. In addition, knockdown of TCF3 can inhibit BL cell proliferation, reduce cell viability and promote cell apoptosis, retain the cell cycle in the G0/G1 phase, and inhibit the expression of Akt/mTOR pathway-related proteins (p-Akt and p-mTOR). When miR-101 was overexpressed, the results were the same as when TCF3 was knocked down. Moreover, we used Co-immunoprecipitation (Co-IP) to detect the interaction between TCF3 and HDAC3, and performed the Chromatin immunoprecipitation (ChIP) experiment to detect the enrichment of TCF3 and HDAC3 in the promoter region of miR-101. We found that TCF3 can interact with HDAC3 and is enriched in the miR-101 promoter region. In conclusion, TCF3 combined with HDAC3 down-regulates the expression of miR-101, thereby promoting the proliferation of BL cells and inhibiting their apoptosis.http://dx.doi.org/10.1080/21655979.2021.1977557burkitt lymphomatranscription factor tcf3hdac3mir-101 |
| spellingShingle | Lihua Dong Jingjing Huang Peng Zu Jing Liu Xue Gao Jianwei Du Yufu Li Transcription factor 3 (TCF3) combined with histone deacetylase 3 (HDAC3) down-regulates microRNA-101 to promote Burkitt lymphoma cell proliferation and inhibit apoptosis Bioengineered burkitt lymphoma transcription factor tcf3 hdac3 mir-101 |
| title | Transcription factor 3 (TCF3) combined with histone deacetylase 3 (HDAC3) down-regulates microRNA-101 to promote Burkitt lymphoma cell proliferation and inhibit apoptosis |
| title_full | Transcription factor 3 (TCF3) combined with histone deacetylase 3 (HDAC3) down-regulates microRNA-101 to promote Burkitt lymphoma cell proliferation and inhibit apoptosis |
| title_fullStr | Transcription factor 3 (TCF3) combined with histone deacetylase 3 (HDAC3) down-regulates microRNA-101 to promote Burkitt lymphoma cell proliferation and inhibit apoptosis |
| title_full_unstemmed | Transcription factor 3 (TCF3) combined with histone deacetylase 3 (HDAC3) down-regulates microRNA-101 to promote Burkitt lymphoma cell proliferation and inhibit apoptosis |
| title_short | Transcription factor 3 (TCF3) combined with histone deacetylase 3 (HDAC3) down-regulates microRNA-101 to promote Burkitt lymphoma cell proliferation and inhibit apoptosis |
| title_sort | transcription factor 3 tcf3 combined with histone deacetylase 3 hdac3 down regulates microrna 101 to promote burkitt lymphoma cell proliferation and inhibit apoptosis |
| topic | burkitt lymphoma transcription factor tcf3 hdac3 mir-101 |
| url | http://dx.doi.org/10.1080/21655979.2021.1977557 |
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