Establishment of ultrasound-responsive SonoBacteriaBot for targeted drug delivery and controlled release
Bacteria-driven biohybrid microbots have shown great potential in cancer treatment. However, how precisely controlling drug release at the tumor site is still an issue. To overcome the limitation of this system, we proposed the ultrasound-responsive SonoBacteriaBot (DOX-PFP-PLGA@EcM). Doxorubicin (D...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2023-02-01
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| Series: | Frontiers in Bioengineering and Biotechnology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fbioe.2023.1144963/full |
| _version_ | 1797895422674468864 |
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| author | Meng Du Meng Du Ting Wang Ting Wang Renjie Feng Renjie Feng Penghui Zeng Penghui Zeng Zhiyi Chen Zhiyi Chen Zhiyi Chen |
| author_facet | Meng Du Meng Du Ting Wang Ting Wang Renjie Feng Renjie Feng Penghui Zeng Penghui Zeng Zhiyi Chen Zhiyi Chen Zhiyi Chen |
| author_sort | Meng Du |
| collection | DOAJ |
| description | Bacteria-driven biohybrid microbots have shown great potential in cancer treatment. However, how precisely controlling drug release at the tumor site is still an issue. To overcome the limitation of this system, we proposed the ultrasound-responsive SonoBacteriaBot (DOX-PFP-PLGA@EcM). Doxorubicin (DOX) and perfluoro-n-pentane (PFP) were encapsulated in polylactic acid-glycolic acid (PLGA) to form ultrasound-responsive DOX-PFP-PLGA nanodroplets. Then, DOX-PFP-PLGA@EcM is created by DOX-PFP-PLGA amide-bonded to the surface of E. coli MG1655 (EcM). The DOX-PFP-PLGA@EcM was proved to have the characteristics of high tumor-targeting efficiency, controlled drug release capability, and ultrasound imaging. Based on the acoustic phase change function of nanodroplets, DOX-PFP-PLGA@EcM enhance the signal of US imaging after ultrasound irradiation. Meanwhile, the DOX loaded into DOX-PFP-PLGA@EcM can be released. After being intravenously injected, DOX-PFP-PLGA@EcM can efficiently accumulate in tumors without causing harm to critical organs. In conclusion, the SonoBacteriaBot has significant benefits in real-time monitoring and controlled drug release, which has significant potential applications for therapeutic drug delivery in clinical settings. |
| first_indexed | 2024-04-10T07:25:23Z |
| format | Article |
| id | doaj.art-a9010a8aa4e5425c803b4aa88129d0b3 |
| institution | Directory Open Access Journal |
| issn | 2296-4185 |
| language | English |
| last_indexed | 2024-04-10T07:25:23Z |
| publishDate | 2023-02-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Bioengineering and Biotechnology |
| spelling | doaj.art-a9010a8aa4e5425c803b4aa88129d0b32023-02-24T07:40:34ZengFrontiers Media S.A.Frontiers in Bioengineering and Biotechnology2296-41852023-02-011110.3389/fbioe.2023.11449631144963Establishment of ultrasound-responsive SonoBacteriaBot for targeted drug delivery and controlled releaseMeng Du0Meng Du1Ting Wang2Ting Wang3Renjie Feng4Renjie Feng5Penghui Zeng6Penghui Zeng7Zhiyi Chen8Zhiyi Chen9Zhiyi Chen10The First Affiliated Hospital, Medical Imaging Centre, Hengyang Medical School, University of South China, Hengyang, Hunan, ChinaInstitute of Medical Imaging, Hengyang Medical School, University of South China, Hengyang, ChinaThe First Affiliated Hospital, Medical Imaging Centre, Hengyang Medical School, University of South China, Hengyang, Hunan, ChinaInstitute of Medical Imaging, Hengyang Medical School, University of South China, Hengyang, ChinaInstitute of Medical Imaging, Hengyang Medical School, University of South China, Hengyang, ChinaThe Seventh Affiliated Hospital, Hunan Veterans Administration Hospital, Hengyang Medical School, University of South China, Changsha, Hunan, ChinaThe First Affiliated Hospital, Medical Imaging Centre, Hengyang Medical School, University of South China, Hengyang, Hunan, ChinaInstitute of Medical Imaging, Hengyang Medical School, University of South China, Hengyang, ChinaThe First Affiliated Hospital, Medical Imaging Centre, Hengyang Medical School, University of South China, Hengyang, Hunan, ChinaInstitute of Medical Imaging, Hengyang Medical School, University of South China, Hengyang, ChinaThe Seventh Affiliated Hospital, Hunan Veterans Administration Hospital, Hengyang Medical School, University of South China, Changsha, Hunan, ChinaBacteria-driven biohybrid microbots have shown great potential in cancer treatment. However, how precisely controlling drug release at the tumor site is still an issue. To overcome the limitation of this system, we proposed the ultrasound-responsive SonoBacteriaBot (DOX-PFP-PLGA@EcM). Doxorubicin (DOX) and perfluoro-n-pentane (PFP) were encapsulated in polylactic acid-glycolic acid (PLGA) to form ultrasound-responsive DOX-PFP-PLGA nanodroplets. Then, DOX-PFP-PLGA@EcM is created by DOX-PFP-PLGA amide-bonded to the surface of E. coli MG1655 (EcM). The DOX-PFP-PLGA@EcM was proved to have the characteristics of high tumor-targeting efficiency, controlled drug release capability, and ultrasound imaging. Based on the acoustic phase change function of nanodroplets, DOX-PFP-PLGA@EcM enhance the signal of US imaging after ultrasound irradiation. Meanwhile, the DOX loaded into DOX-PFP-PLGA@EcM can be released. After being intravenously injected, DOX-PFP-PLGA@EcM can efficiently accumulate in tumors without causing harm to critical organs. In conclusion, the SonoBacteriaBot has significant benefits in real-time monitoring and controlled drug release, which has significant potential applications for therapeutic drug delivery in clinical settings.https://www.frontiersin.org/articles/10.3389/fbioe.2023.1144963/fullultrasounddrug deliverycontrolled releasebiohybrid microbotsbacteria |
| spellingShingle | Meng Du Meng Du Ting Wang Ting Wang Renjie Feng Renjie Feng Penghui Zeng Penghui Zeng Zhiyi Chen Zhiyi Chen Zhiyi Chen Establishment of ultrasound-responsive SonoBacteriaBot for targeted drug delivery and controlled release Frontiers in Bioengineering and Biotechnology ultrasound drug delivery controlled release biohybrid microbots bacteria |
| title | Establishment of ultrasound-responsive SonoBacteriaBot for targeted drug delivery and controlled release |
| title_full | Establishment of ultrasound-responsive SonoBacteriaBot for targeted drug delivery and controlled release |
| title_fullStr | Establishment of ultrasound-responsive SonoBacteriaBot for targeted drug delivery and controlled release |
| title_full_unstemmed | Establishment of ultrasound-responsive SonoBacteriaBot for targeted drug delivery and controlled release |
| title_short | Establishment of ultrasound-responsive SonoBacteriaBot for targeted drug delivery and controlled release |
| title_sort | establishment of ultrasound responsive sonobacteriabot for targeted drug delivery and controlled release |
| topic | ultrasound drug delivery controlled release biohybrid microbots bacteria |
| url | https://www.frontiersin.org/articles/10.3389/fbioe.2023.1144963/full |
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