TRPC3 is a major contributor to functional heterogeneity of cerebellar Purkinje cells
Despite the canonical homogeneous character of its organization, the cerebellum plays differential computational roles in distinct sensorimotor behaviors. Previously, we showed that Purkinje cell (PC) activity differs between zebrin-negative (Z–) and zebrin-positive (Z+) modules (Zhou et al., 2014)....
| Main Authors: | , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
eLife Sciences Publications Ltd
2019-09-01
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| Series: | eLife |
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| Online Access: | https://elifesciences.org/articles/45590 |
| _version_ | 1818023967863078912 |
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| author | Bin Wu François GC Blot Aaron Benson Wong Catarina Osório Youri Adolfs R Jeroen Pasterkamp Jana Hartmann Esther BE Becker Henk-Jan Boele Chris I De Zeeuw Martijn Schonewille |
| author_facet | Bin Wu François GC Blot Aaron Benson Wong Catarina Osório Youri Adolfs R Jeroen Pasterkamp Jana Hartmann Esther BE Becker Henk-Jan Boele Chris I De Zeeuw Martijn Schonewille |
| author_sort | Bin Wu |
| collection | DOAJ |
| description | Despite the canonical homogeneous character of its organization, the cerebellum plays differential computational roles in distinct sensorimotor behaviors. Previously, we showed that Purkinje cell (PC) activity differs between zebrin-negative (Z–) and zebrin-positive (Z+) modules (Zhou et al., 2014). Here, using gain-of-function and loss-of-function mouse models, we show that transient receptor potential cation channel C3 (TRPC3) controls the simple spike activity of Z–, but not Z+ PCs. In addition, TRPC3 regulates complex spike rate and their interaction with simple spikes, exclusively in Z– PCs. At the behavioral level, TRPC3 loss-of-function mice show impaired eyeblink conditioning, which is related to Z– modules, whereas compensatory eye movement adaptation, linked to Z+ modules, is intact. Together, our results indicate that TRPC3 is a major contributor to the cellular heterogeneity that introduces distinct physiological properties in PCs, conjuring functional heterogeneity in cerebellar sensorimotor integration. |
| first_indexed | 2024-12-10T03:52:44Z |
| format | Article |
| id | doaj.art-a9026aa4734a4afca2461feffd8a1e97 |
| institution | Directory Open Access Journal |
| issn | 2050-084X |
| language | English |
| last_indexed | 2024-12-10T03:52:44Z |
| publishDate | 2019-09-01 |
| publisher | eLife Sciences Publications Ltd |
| record_format | Article |
| series | eLife |
| spelling | doaj.art-a9026aa4734a4afca2461feffd8a1e972022-12-22T02:03:12ZengeLife Sciences Publications LtdeLife2050-084X2019-09-01810.7554/eLife.45590TRPC3 is a major contributor to functional heterogeneity of cerebellar Purkinje cellsBin Wu0https://orcid.org/0000-0003-4198-1661François GC Blot1Aaron Benson Wong2https://orcid.org/0000-0003-1650-2710Catarina Osório3Youri Adolfs4R Jeroen Pasterkamp5https://orcid.org/0000-0003-1631-6440Jana Hartmann6Esther BE Becker7https://orcid.org/0000-0002-5238-4902Henk-Jan Boele8Chris I De Zeeuw9https://orcid.org/0000-0001-5628-8187Martijn Schonewille10https://orcid.org/0000-0002-2675-1393Department of Neuroscience, Erasmus Medical Center, Rotterdam, NetherlandsDepartment of Neuroscience, Erasmus Medical Center, Rotterdam, NetherlandsDepartment of Neuroscience, Erasmus Medical Center, Rotterdam, NetherlandsDepartment of Neuroscience, Erasmus Medical Center, Rotterdam, NetherlandsDepartment of Translational Neuroscience, University Medical Center Utrecht, Utrecht University, Utrecht, NetherlandsDepartment of Translational Neuroscience, University Medical Center Utrecht, Utrecht University, Utrecht, NetherlandsInstitute of Neuroscience, Technical University Munich, Munich, GermanyDepartment of Physiology, Anatomy and Genetics, University of Oxford, Oxford, United KingdomDepartment of Neuroscience, Erasmus Medical Center, Rotterdam, NetherlandsDepartment of Neuroscience, Erasmus Medical Center, Rotterdam, Netherlands; Netherlands Institute for Neuroscience, Royal Dutch Academy for Arts and Sciences, Amsterdam, NetherlandsDepartment of Neuroscience, Erasmus Medical Center, Rotterdam, NetherlandsDespite the canonical homogeneous character of its organization, the cerebellum plays differential computational roles in distinct sensorimotor behaviors. Previously, we showed that Purkinje cell (PC) activity differs between zebrin-negative (Z–) and zebrin-positive (Z+) modules (Zhou et al., 2014). Here, using gain-of-function and loss-of-function mouse models, we show that transient receptor potential cation channel C3 (TRPC3) controls the simple spike activity of Z–, but not Z+ PCs. In addition, TRPC3 regulates complex spike rate and their interaction with simple spikes, exclusively in Z– PCs. At the behavioral level, TRPC3 loss-of-function mice show impaired eyeblink conditioning, which is related to Z– modules, whereas compensatory eye movement adaptation, linked to Z+ modules, is intact. Together, our results indicate that TRPC3 is a major contributor to the cellular heterogeneity that introduces distinct physiological properties in PCs, conjuring functional heterogeneity in cerebellar sensorimotor integration.https://elifesciences.org/articles/45590cerebellar functionPurkinje cellzebrin/aldolase CTRPC3cellular heterogeneityspiking activity |
| spellingShingle | Bin Wu François GC Blot Aaron Benson Wong Catarina Osório Youri Adolfs R Jeroen Pasterkamp Jana Hartmann Esther BE Becker Henk-Jan Boele Chris I De Zeeuw Martijn Schonewille TRPC3 is a major contributor to functional heterogeneity of cerebellar Purkinje cells eLife cerebellar function Purkinje cell zebrin/aldolase C TRPC3 cellular heterogeneity spiking activity |
| title | TRPC3 is a major contributor to functional heterogeneity of cerebellar Purkinje cells |
| title_full | TRPC3 is a major contributor to functional heterogeneity of cerebellar Purkinje cells |
| title_fullStr | TRPC3 is a major contributor to functional heterogeneity of cerebellar Purkinje cells |
| title_full_unstemmed | TRPC3 is a major contributor to functional heterogeneity of cerebellar Purkinje cells |
| title_short | TRPC3 is a major contributor to functional heterogeneity of cerebellar Purkinje cells |
| title_sort | trpc3 is a major contributor to functional heterogeneity of cerebellar purkinje cells |
| topic | cerebellar function Purkinje cell zebrin/aldolase C TRPC3 cellular heterogeneity spiking activity |
| url | https://elifesciences.org/articles/45590 |
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