Combination Interactive Effects of Gongronema latifolium Leaves and Picralima nitida Seeds Extracts on Glucose Tolerance
This study evaluated the combination interactive effects of G. latifolium leaves and P. nitida seed extracts using a metabolic glucose tolerance test. The plant samples were extracted separately using cold maceration and their acute toxicities were determined. Dose-response glucose tolerant tests of...
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| Format: | Article |
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State Islamic University Sunan Kalijaga
2023-03-01
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| Series: | Biology, Medicine & Natural Product Chemistry |
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| Online Access: | https://sciencebiology.org/index.php/BIOMEDICH/article/view/291 |
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| author | Maureen Ifeyinwa Egbuniwe Harrison Anezichukwu Ozoani Amara Anwuchaepe Ajaghaku Ikechukwu Sonne Mbagwu Uchechukwu Harrison Orji Daniel Lotanna Ajaghaku |
| author_facet | Maureen Ifeyinwa Egbuniwe Harrison Anezichukwu Ozoani Amara Anwuchaepe Ajaghaku Ikechukwu Sonne Mbagwu Uchechukwu Harrison Orji Daniel Lotanna Ajaghaku |
| author_sort | Maureen Ifeyinwa Egbuniwe |
| collection | DOAJ |
| description | This study evaluated the combination interactive effects of G. latifolium leaves and P. nitida seed extracts using a metabolic glucose tolerance test. The plant samples were extracted separately using cold maceration and their acute toxicities were determined. Dose-response glucose tolerant tests of both plants were done using a 2 g/kg glucose load monitored over 0 – 1h. A 41% effect isobologram was used to determine the needed dose combinations according to the principle of Loewe’s additivity model. The glucose tolerant tests of dose pairs of the combined extracts were evaluated and their combination indices were calculated to determine the nature of their interaction. The ED41 of G. latifolium (GL) and P. nitida (PN) were 180 mg/kg and 254 mg/kg respectively. The percentage reductions of GL:PN (50:160); GL:PN (100:90); and GL:PN (150:30) dose pairs were 48.23, 50.76 and 42.99 % respectively. Their combination index were calculated to be 0.91, 0.91 and 0.95 respectively - an indication of synergistic interaction. Findings from this study validate the combined use of G. latifolium leaves and P. nitida seeds in folkloric medicine. However, combining the extracts of G. latifolium: P. nitida in the dose ratios of 50:160, 100:90 and 150:30 mg/kg gave the best dose pairs with synergistic outcome. |
| first_indexed | 2024-03-12T15:06:37Z |
| format | Article |
| id | doaj.art-a9033d10cccc443888d4cd88d6fd2612 |
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| issn | 2089-6514 2540-9328 |
| language | English |
| last_indexed | 2024-03-12T15:06:37Z |
| publishDate | 2023-03-01 |
| publisher | State Islamic University Sunan Kalijaga |
| record_format | Article |
| series | Biology, Medicine & Natural Product Chemistry |
| spelling | doaj.art-a9033d10cccc443888d4cd88d6fd26122023-08-12T13:06:44ZengState Islamic University Sunan KalijagaBiology, Medicine & Natural Product Chemistry2089-65142540-93282023-03-0112125926610.14421/biomedich.2023.121.259-266177Combination Interactive Effects of Gongronema latifolium Leaves and Picralima nitida Seeds Extracts on Glucose ToleranceMaureen Ifeyinwa Egbuniwe0Harrison Anezichukwu Ozoani1Amara Anwuchaepe Ajaghaku2Ikechukwu Sonne Mbagwu3Uchechukwu Harrison Orji4Daniel Lotanna Ajaghaku5Enugu State University of Science and TechnologyEnugu State University of Science and TechnologyChukwuemeka Odumegwu Ojukwu UniversityNnamdi Azikiwe University AwkaNnamdi Azikiwe University AwkaEnugu State University of Science and TechnologyThis study evaluated the combination interactive effects of G. latifolium leaves and P. nitida seed extracts using a metabolic glucose tolerance test. The plant samples were extracted separately using cold maceration and their acute toxicities were determined. Dose-response glucose tolerant tests of both plants were done using a 2 g/kg glucose load monitored over 0 – 1h. A 41% effect isobologram was used to determine the needed dose combinations according to the principle of Loewe’s additivity model. The glucose tolerant tests of dose pairs of the combined extracts were evaluated and their combination indices were calculated to determine the nature of their interaction. The ED41 of G. latifolium (GL) and P. nitida (PN) were 180 mg/kg and 254 mg/kg respectively. The percentage reductions of GL:PN (50:160); GL:PN (100:90); and GL:PN (150:30) dose pairs were 48.23, 50.76 and 42.99 % respectively. Their combination index were calculated to be 0.91, 0.91 and 0.95 respectively - an indication of synergistic interaction. Findings from this study validate the combined use of G. latifolium leaves and P. nitida seeds in folkloric medicine. However, combining the extracts of G. latifolium: P. nitida in the dose ratios of 50:160, 100:90 and 150:30 mg/kg gave the best dose pairs with synergistic outcome.https://sciencebiology.org/index.php/BIOMEDICH/article/view/291combination studiesgongronema latifoliumglucose tolerancepicralima nitida |
| spellingShingle | Maureen Ifeyinwa Egbuniwe Harrison Anezichukwu Ozoani Amara Anwuchaepe Ajaghaku Ikechukwu Sonne Mbagwu Uchechukwu Harrison Orji Daniel Lotanna Ajaghaku Combination Interactive Effects of Gongronema latifolium Leaves and Picralima nitida Seeds Extracts on Glucose Tolerance Biology, Medicine & Natural Product Chemistry combination studies gongronema latifolium glucose tolerance picralima nitida |
| title | Combination Interactive Effects of Gongronema latifolium Leaves and Picralima nitida Seeds Extracts on Glucose Tolerance |
| title_full | Combination Interactive Effects of Gongronema latifolium Leaves and Picralima nitida Seeds Extracts on Glucose Tolerance |
| title_fullStr | Combination Interactive Effects of Gongronema latifolium Leaves and Picralima nitida Seeds Extracts on Glucose Tolerance |
| title_full_unstemmed | Combination Interactive Effects of Gongronema latifolium Leaves and Picralima nitida Seeds Extracts on Glucose Tolerance |
| title_short | Combination Interactive Effects of Gongronema latifolium Leaves and Picralima nitida Seeds Extracts on Glucose Tolerance |
| title_sort | combination interactive effects of gongronema latifolium leaves and picralima nitida seeds extracts on glucose tolerance |
| topic | combination studies gongronema latifolium glucose tolerance picralima nitida |
| url | https://sciencebiology.org/index.php/BIOMEDICH/article/view/291 |
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