Monophosphoryl Lipid A and Poly I:C Combination Adjuvant Promoted Ovalbumin-Specific Cell Mediated Immunity in Mice Model
Induction of antigen-specific cell-mediated immunity (CMI), as well as humoral immunity, is critical for successful vaccination against various type of pathogens. Toll-like receptor (TLR) agonists have been developed as adjuvants to promote vaccine efficacy and induce appropriate immune responses. M...
| Main Authors: | , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2021-09-01
|
| Series: | Biology |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2079-7737/10/9/908 |
| _version_ | 1797520200939077632 |
|---|---|
| author | So Yeon Ahn Chau Thuy Tien Le Eun-Ju Ko |
| author_facet | So Yeon Ahn Chau Thuy Tien Le Eun-Ju Ko |
| author_sort | So Yeon Ahn |
| collection | DOAJ |
| description | Induction of antigen-specific cell-mediated immunity (CMI), as well as humoral immunity, is critical for successful vaccination against various type of pathogens. Toll-like receptor (TLR) agonists have been developed as adjuvants to promote vaccine efficacy and induce appropriate immune responses. Monophosphoryl lipid A (MPL); a TLR4 agonist, and Poly I:C; a TLR3 agonist, are known as a strong immuno-stimulator which induce Th1 response. Many studies proved and compared the efficacy of each adjuvant, but no study has investigated the combination of them. Using ovalbumin protein antigen, MPL+Poly I:C combination induced more effective antigen-specific CMI response than single adjuvants. Production of inflammatory cytokines, recruitment of innate immune cells and antigen-specific CD4/CD8 memory T cell at the immunized site had been significantly enhanced by MPL+Poly I:C combination. Moreover, MPL+Poly I:C combination enhanced ovalbumin-specific serum IgG, IgG1, and IgG2c production and proliferative function of CD4 and CD8 T cells after in vitro ovalbumin peptide stimulation. Taken together, these data suggest that the combination of MPL and Poly I:C has a potency as a CMI-inducing vaccine adjuvant with synergistically increased effects. |
| first_indexed | 2024-03-10T07:53:30Z |
| format | Article |
| id | doaj.art-a9052bc43f7740f590cec53f06b5a157 |
| institution | Directory Open Access Journal |
| issn | 2079-7737 |
| language | English |
| last_indexed | 2024-03-10T07:53:30Z |
| publishDate | 2021-09-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Biology |
| spelling | doaj.art-a9052bc43f7740f590cec53f06b5a1572023-11-22T12:06:04ZengMDPI AGBiology2079-77372021-09-0110990810.3390/biology10090908Monophosphoryl Lipid A and Poly I:C Combination Adjuvant Promoted Ovalbumin-Specific Cell Mediated Immunity in Mice ModelSo Yeon Ahn0Chau Thuy Tien Le1Eun-Ju Ko2Department of Veterinary Medicine, College of Veterinary Medicine, Jeju National University, Jeju 63243, KoreaInterdisciplinary Graduate Program in Advanced Convergence Technology & Science, Jeju National University, Jeju 63243, KoreaDepartment of Veterinary Medicine, College of Veterinary Medicine, Jeju National University, Jeju 63243, KoreaInduction of antigen-specific cell-mediated immunity (CMI), as well as humoral immunity, is critical for successful vaccination against various type of pathogens. Toll-like receptor (TLR) agonists have been developed as adjuvants to promote vaccine efficacy and induce appropriate immune responses. Monophosphoryl lipid A (MPL); a TLR4 agonist, and Poly I:C; a TLR3 agonist, are known as a strong immuno-stimulator which induce Th1 response. Many studies proved and compared the efficacy of each adjuvant, but no study has investigated the combination of them. Using ovalbumin protein antigen, MPL+Poly I:C combination induced more effective antigen-specific CMI response than single adjuvants. Production of inflammatory cytokines, recruitment of innate immune cells and antigen-specific CD4/CD8 memory T cell at the immunized site had been significantly enhanced by MPL+Poly I:C combination. Moreover, MPL+Poly I:C combination enhanced ovalbumin-specific serum IgG, IgG1, and IgG2c production and proliferative function of CD4 and CD8 T cells after in vitro ovalbumin peptide stimulation. Taken together, these data suggest that the combination of MPL and Poly I:C has a potency as a CMI-inducing vaccine adjuvant with synergistically increased effects.https://www.mdpi.com/2079-7737/10/9/908cell-mediated immunitymemory T celldendritic cellmonophosphoryl lipid APoly I:C |
| spellingShingle | So Yeon Ahn Chau Thuy Tien Le Eun-Ju Ko Monophosphoryl Lipid A and Poly I:C Combination Adjuvant Promoted Ovalbumin-Specific Cell Mediated Immunity in Mice Model Biology cell-mediated immunity memory T cell dendritic cell monophosphoryl lipid A Poly I:C |
| title | Monophosphoryl Lipid A and Poly I:C Combination Adjuvant Promoted Ovalbumin-Specific Cell Mediated Immunity in Mice Model |
| title_full | Monophosphoryl Lipid A and Poly I:C Combination Adjuvant Promoted Ovalbumin-Specific Cell Mediated Immunity in Mice Model |
| title_fullStr | Monophosphoryl Lipid A and Poly I:C Combination Adjuvant Promoted Ovalbumin-Specific Cell Mediated Immunity in Mice Model |
| title_full_unstemmed | Monophosphoryl Lipid A and Poly I:C Combination Adjuvant Promoted Ovalbumin-Specific Cell Mediated Immunity in Mice Model |
| title_short | Monophosphoryl Lipid A and Poly I:C Combination Adjuvant Promoted Ovalbumin-Specific Cell Mediated Immunity in Mice Model |
| title_sort | monophosphoryl lipid a and poly i c combination adjuvant promoted ovalbumin specific cell mediated immunity in mice model |
| topic | cell-mediated immunity memory T cell dendritic cell monophosphoryl lipid A Poly I:C |
| url | https://www.mdpi.com/2079-7737/10/9/908 |
| work_keys_str_mv | AT soyeonahn monophosphoryllipidaandpolyiccombinationadjuvantpromotedovalbuminspecificcellmediatedimmunityinmicemodel AT chauthuytienle monophosphoryllipidaandpolyiccombinationadjuvantpromotedovalbuminspecificcellmediatedimmunityinmicemodel AT eunjuko monophosphoryllipidaandpolyiccombinationadjuvantpromotedovalbuminspecificcellmediatedimmunityinmicemodel |