Leaf gel from several Aloe species shows anti-inflammatory properties through the inhibition of lipopolysaccharide (LPS) mediated activation of Toll-like receptor 4 (TLR4) signaling
Background: Gel extracted from the succulent inner leaf mesophyll of species from the plant genus Aloe have been used in traditional medicine for centuries as a topical treatment for a wide range of skin conditions. Aim of the study: The aim of this study was to investigate the potential anti-inflam...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2023-02-01
|
| Series: | Phytomedicine Plus |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2667031322001762 |
| _version_ | 1828041966208679936 |
|---|---|
| author | Louise Isager Ahl Mia Prehn Schoeneburg Lisa Harth Christopher J. Barnes Anders Woetmann Nina Rønsted |
| author_facet | Louise Isager Ahl Mia Prehn Schoeneburg Lisa Harth Christopher J. Barnes Anders Woetmann Nina Rønsted |
| author_sort | Louise Isager Ahl |
| collection | DOAJ |
| description | Background: Gel extracted from the succulent inner leaf mesophyll of species from the plant genus Aloe have been used in traditional medicine for centuries as a topical treatment for a wide range of skin conditions. Aim of the study: The aim of this study was to investigate the potential anti-inflammatory properties of leaf gel from 23 different Aloe species. In this study we have focused on the pathways activated by the toll-like receptors TLR4 (activated by LPS), and TLR2/TLR6 (activated by FSL-1) by measuring transcriptional activity of NF-κB. Materials and methods: Gel extractions from the inner leaf mesophyll material of 23 different Aloe species, pure acemannan obtained from Aloe vera, and pH. Eur. reference material from Aloe vera and Aloe ferox, were tested on the cell line J774-Dual. NF-κB activity was measured after 24 h. Results: We found that 8 out of the 23 tested Aloe gel extracts significantly decreased LPS-induced NF-κB-activity, but no significant effect was seen with FSL-1. Conclusion: Based on these findings we can confirm that extracted gel from some of the tested Aloe species but not all, have anti-inflammatory properties. |
| first_indexed | 2024-04-10T17:24:01Z |
| format | Article |
| id | doaj.art-a906913448114172981feda0abd2736e |
| institution | Directory Open Access Journal |
| issn | 2667-0313 |
| language | English |
| last_indexed | 2024-04-10T17:24:01Z |
| publishDate | 2023-02-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Phytomedicine Plus |
| spelling | doaj.art-a906913448114172981feda0abd2736e2023-02-05T04:17:32ZengElsevierPhytomedicine Plus2667-03132023-02-0131100397Leaf gel from several Aloe species shows anti-inflammatory properties through the inhibition of lipopolysaccharide (LPS) mediated activation of Toll-like receptor 4 (TLR4) signalingLouise Isager Ahl0Mia Prehn Schoeneburg1Lisa Harth2Christopher J. Barnes3Anders Woetmann4Nina Rønsted5Natural History Museum of Denmark, University of Copenhagen, Øster Voldgade 5-7, 2100 Copenhagen, Denmark; Corresponding author at: Natural History Museum of Denmark, University of Copenhagen, Øster Voldgade 5-7, 2100, Copenhagen, Denmark.Natural History Museum of Denmark, University of Copenhagen, Øster Voldgade 5-7, 2100 Copenhagen, DenmarkThe LEO Skin Immunology Research Center, Department of Immunology and Microbiology, University of Copenhagen, Blegdamsvej 3B, 2200 København N, 07 Bygning 7, Building: 07-12-76, DenmarkGlobe Institute, University of Copenhagen, Øster Voldgade 5-7, 1350 København K, 2100, Copenhagen, DenmarkThe LEO Skin Immunology Research Center, Department of Immunology and Microbiology, University of Copenhagen, Blegdamsvej 3B, 2200 København N, 07 Bygning 7, Building: 07-12-76, DenmarkNatural History Museum of Denmark, University of Copenhagen, Øster Voldgade 5-7, 2100 Copenhagen, Denmark; National Tropical Botanical Garden, 3530 Papalina Road, Kalāheo, Hawai‘i, 96741, United States of AmericaBackground: Gel extracted from the succulent inner leaf mesophyll of species from the plant genus Aloe have been used in traditional medicine for centuries as a topical treatment for a wide range of skin conditions. Aim of the study: The aim of this study was to investigate the potential anti-inflammatory properties of leaf gel from 23 different Aloe species. In this study we have focused on the pathways activated by the toll-like receptors TLR4 (activated by LPS), and TLR2/TLR6 (activated by FSL-1) by measuring transcriptional activity of NF-κB. Materials and methods: Gel extractions from the inner leaf mesophyll material of 23 different Aloe species, pure acemannan obtained from Aloe vera, and pH. Eur. reference material from Aloe vera and Aloe ferox, were tested on the cell line J774-Dual. NF-κB activity was measured after 24 h. Results: We found that 8 out of the 23 tested Aloe gel extracts significantly decreased LPS-induced NF-κB-activity, but no significant effect was seen with FSL-1. Conclusion: Based on these findings we can confirm that extracted gel from some of the tested Aloe species but not all, have anti-inflammatory properties.http://www.sciencedirect.com/science/article/pii/S2667031322001762Wound healingTraditional medicineAfricaPolysaccharidesMacrophagesInflammation |
| spellingShingle | Louise Isager Ahl Mia Prehn Schoeneburg Lisa Harth Christopher J. Barnes Anders Woetmann Nina Rønsted Leaf gel from several Aloe species shows anti-inflammatory properties through the inhibition of lipopolysaccharide (LPS) mediated activation of Toll-like receptor 4 (TLR4) signaling Phytomedicine Plus Wound healing Traditional medicine Africa Polysaccharides Macrophages Inflammation |
| title | Leaf gel from several Aloe species shows anti-inflammatory properties through the inhibition of lipopolysaccharide (LPS) mediated activation of Toll-like receptor 4 (TLR4) signaling |
| title_full | Leaf gel from several Aloe species shows anti-inflammatory properties through the inhibition of lipopolysaccharide (LPS) mediated activation of Toll-like receptor 4 (TLR4) signaling |
| title_fullStr | Leaf gel from several Aloe species shows anti-inflammatory properties through the inhibition of lipopolysaccharide (LPS) mediated activation of Toll-like receptor 4 (TLR4) signaling |
| title_full_unstemmed | Leaf gel from several Aloe species shows anti-inflammatory properties through the inhibition of lipopolysaccharide (LPS) mediated activation of Toll-like receptor 4 (TLR4) signaling |
| title_short | Leaf gel from several Aloe species shows anti-inflammatory properties through the inhibition of lipopolysaccharide (LPS) mediated activation of Toll-like receptor 4 (TLR4) signaling |
| title_sort | leaf gel from several aloe species shows anti inflammatory properties through the inhibition of lipopolysaccharide lps mediated activation of toll like receptor 4 tlr4 signaling |
| topic | Wound healing Traditional medicine Africa Polysaccharides Macrophages Inflammation |
| url | http://www.sciencedirect.com/science/article/pii/S2667031322001762 |
| work_keys_str_mv | AT louiseisagerahl leafgelfromseveralaloespeciesshowsantiinflammatorypropertiesthroughtheinhibitionoflipopolysaccharidelpsmediatedactivationoftolllikereceptor4tlr4signaling AT miaprehnschoeneburg leafgelfromseveralaloespeciesshowsantiinflammatorypropertiesthroughtheinhibitionoflipopolysaccharidelpsmediatedactivationoftolllikereceptor4tlr4signaling AT lisaharth leafgelfromseveralaloespeciesshowsantiinflammatorypropertiesthroughtheinhibitionoflipopolysaccharidelpsmediatedactivationoftolllikereceptor4tlr4signaling AT christopherjbarnes leafgelfromseveralaloespeciesshowsantiinflammatorypropertiesthroughtheinhibitionoflipopolysaccharidelpsmediatedactivationoftolllikereceptor4tlr4signaling AT anderswoetmann leafgelfromseveralaloespeciesshowsantiinflammatorypropertiesthroughtheinhibitionoflipopolysaccharidelpsmediatedactivationoftolllikereceptor4tlr4signaling AT ninarønsted leafgelfromseveralaloespeciesshowsantiinflammatorypropertiesthroughtheinhibitionoflipopolysaccharidelpsmediatedactivationoftolllikereceptor4tlr4signaling |