Bacterial Diversity and Community Composition Distribution in Cold-Desert Habitats of Qinghai–Tibet Plateau, China
Bacterial communities in cold-desert habitats play an important ecological role. However, the variation in bacterial diversity and community composition of the cold-desert ecosystem in Qinghai–Tibet Plateau remains unknown. To fill this scientific gape, Illumina MiSeq sequencing was performed on 15...
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| Format: | Article |
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MDPI AG
2021-01-01
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| Series: | Microorganisms |
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| Online Access: | https://www.mdpi.com/2076-2607/9/2/262 |
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| author | Wei Zhang Ali Bahadur Wasim Sajjad Gaosen Zhang Fahad Nasir Binglin Zhang Xiukun Wu Guangxiu Liu Tuo Chen |
| author_facet | Wei Zhang Ali Bahadur Wasim Sajjad Gaosen Zhang Fahad Nasir Binglin Zhang Xiukun Wu Guangxiu Liu Tuo Chen |
| author_sort | Wei Zhang |
| collection | DOAJ |
| description | Bacterial communities in cold-desert habitats play an important ecological role. However, the variation in bacterial diversity and community composition of the cold-desert ecosystem in Qinghai–Tibet Plateau remains unknown. To fill this scientific gape, Illumina MiSeq sequencing was performed on 15 soil samples collected from different cold-desert habitats, including human-disturbed, vegetation coverage, desert land, and sand dune. The abundance-based coverage estimator, Shannon, and Chao indices showed that the bacterial diversity and abundance of the cold-desert were high. A significant variation reported in the bacterial diversity and community composition across the study area. Proteobacteria accounted for the largest proportion (12.4–55.7%) of all sequences, followed by Actinobacteria (9.2–39.7%), Bacteroidetes (1.8–21.5%), and Chloroflexi (2.7–12.6%). Furthermore, unclassified genera dominated in human-disturbed habitats. The community profiles of GeErMu, HongLiangHe, and CuoNaHu sites were different and metagenomic biomarkers were higher (22) in CuoNaHu sites. Among the soil physicochemical variables, the total nitrogen and electric conductivity significantly influenced the bacterial community structure. In conclusion, this study provides information regarding variation in diversity and composition of bacterial communities and elucidates the association between bacterial community structures and soil physicochemical variables in cold-desert habitats of Qinghai–Tibet Plateau. |
| first_indexed | 2024-03-09T03:29:49Z |
| format | Article |
| id | doaj.art-a90718d7b7eb412ab9cd1b6e641aff77 |
| institution | Directory Open Access Journal |
| issn | 2076-2607 |
| language | English |
| last_indexed | 2024-03-09T03:29:49Z |
| publishDate | 2021-01-01 |
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| record_format | Article |
| series | Microorganisms |
| spelling | doaj.art-a90718d7b7eb412ab9cd1b6e641aff772023-12-03T14:57:17ZengMDPI AGMicroorganisms2076-26072021-01-019226210.3390/microorganisms9020262Bacterial Diversity and Community Composition Distribution in Cold-Desert Habitats of Qinghai–Tibet Plateau, ChinaWei Zhang0Ali Bahadur1Wasim Sajjad2Gaosen Zhang3Fahad Nasir4Binglin Zhang5Xiukun Wu6Guangxiu Liu7Tuo Chen8Key Laboratory of Desert and Desertification, Northwest Institute of Eco-Environment and Resources, Chinese Academy of Sciences, Lanzhou 730000, ChinaKey Laboratory of Extreme Environmental Microbial Resources and Engineering, Lanzhou 730000, ChinaState Key Laboratory of Cryospheric Science, Northwest Institute of Eco-Environment and Resources, Chinese Academy of Sciences, Lanzhou 730000, ChinaKey Laboratory of Desert and Desertification, Northwest Institute of Eco-Environment and Resources, Chinese Academy of Sciences, Lanzhou 730000, ChinaKey Laboratory of Mollisols Agroecology, Northeast Institute of Geography and Agroecology, Chinese Academy of Sciences, Changchun 130102, ChinaKey Laboratory of Extreme Environmental Microbial Resources and Engineering, Lanzhou 730000, ChinaKey Laboratory of Desert and Desertification, Northwest Institute of Eco-Environment and Resources, Chinese Academy of Sciences, Lanzhou 730000, ChinaKey Laboratory of Desert and Desertification, Northwest Institute of Eco-Environment and Resources, Chinese Academy of Sciences, Lanzhou 730000, ChinaState Key Laboratory of Cryospheric Science, Northwest Institute of Eco-Environment and Resources, Chinese Academy of Sciences, Lanzhou 730000, ChinaBacterial communities in cold-desert habitats play an important ecological role. However, the variation in bacterial diversity and community composition of the cold-desert ecosystem in Qinghai–Tibet Plateau remains unknown. To fill this scientific gape, Illumina MiSeq sequencing was performed on 15 soil samples collected from different cold-desert habitats, including human-disturbed, vegetation coverage, desert land, and sand dune. The abundance-based coverage estimator, Shannon, and Chao indices showed that the bacterial diversity and abundance of the cold-desert were high. A significant variation reported in the bacterial diversity and community composition across the study area. Proteobacteria accounted for the largest proportion (12.4–55.7%) of all sequences, followed by Actinobacteria (9.2–39.7%), Bacteroidetes (1.8–21.5%), and Chloroflexi (2.7–12.6%). Furthermore, unclassified genera dominated in human-disturbed habitats. The community profiles of GeErMu, HongLiangHe, and CuoNaHu sites were different and metagenomic biomarkers were higher (22) in CuoNaHu sites. Among the soil physicochemical variables, the total nitrogen and electric conductivity significantly influenced the bacterial community structure. In conclusion, this study provides information regarding variation in diversity and composition of bacterial communities and elucidates the association between bacterial community structures and soil physicochemical variables in cold-desert habitats of Qinghai–Tibet Plateau.https://www.mdpi.com/2076-2607/9/2/262cold-desertproteobacteriaIllumina MiSeq sequencinggeochemical factorsindicator taxahuman activity interference |
| spellingShingle | Wei Zhang Ali Bahadur Wasim Sajjad Gaosen Zhang Fahad Nasir Binglin Zhang Xiukun Wu Guangxiu Liu Tuo Chen Bacterial Diversity and Community Composition Distribution in Cold-Desert Habitats of Qinghai–Tibet Plateau, China Microorganisms cold-desert proteobacteria Illumina MiSeq sequencing geochemical factors indicator taxa human activity interference |
| title | Bacterial Diversity and Community Composition Distribution in Cold-Desert Habitats of Qinghai–Tibet Plateau, China |
| title_full | Bacterial Diversity and Community Composition Distribution in Cold-Desert Habitats of Qinghai–Tibet Plateau, China |
| title_fullStr | Bacterial Diversity and Community Composition Distribution in Cold-Desert Habitats of Qinghai–Tibet Plateau, China |
| title_full_unstemmed | Bacterial Diversity and Community Composition Distribution in Cold-Desert Habitats of Qinghai–Tibet Plateau, China |
| title_short | Bacterial Diversity and Community Composition Distribution in Cold-Desert Habitats of Qinghai–Tibet Plateau, China |
| title_sort | bacterial diversity and community composition distribution in cold desert habitats of qinghai tibet plateau china |
| topic | cold-desert proteobacteria Illumina MiSeq sequencing geochemical factors indicator taxa human activity interference |
| url | https://www.mdpi.com/2076-2607/9/2/262 |
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