Surface Plasmon Resonance Microscopy Based on Total Internal Reflection
Surface plasmon resonance microscopy (SPRM) has been widely employed in biological fields because of its high spatial resolution and label-free detection modality. In this study, SPRM based on total internal reflection (TIR) is studied via a home-built SPRM system, and the principle of imaging of a...
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-02-01
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| Series: | Biosensors |
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| Online Access: | https://www.mdpi.com/2079-6374/13/2/261 |
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| author | Teliang Zhang Xueliang Wang Youjun Zeng Songfeng Huang Xiaoqi Dai Weifu Kong Qian Liu Jiajie Chen Junle Qu Yonghong Shao |
| author_facet | Teliang Zhang Xueliang Wang Youjun Zeng Songfeng Huang Xiaoqi Dai Weifu Kong Qian Liu Jiajie Chen Junle Qu Yonghong Shao |
| author_sort | Teliang Zhang |
| collection | DOAJ |
| description | Surface plasmon resonance microscopy (SPRM) has been widely employed in biological fields because of its high spatial resolution and label-free detection modality. In this study, SPRM based on total internal reflection (TIR) is studied via a home-built SPRM system, and the principle of imaging of a single nanoparticle is analyzed as well. By designing a ring filter and combining it with the deconvolution algorithm in Fourier space, the parabolic tail of the nanoparticle image is removed, in which a spatial resolution of 248 nm is obtained. In addition, we also measured the specific binding between the human IgG antigen and goat anti-human IgG antibody using the TIR-based SPRM. The experimental results have proved that the system can image sparse nanoparticles and monitor biomolecular interactions. |
| first_indexed | 2024-03-11T09:05:13Z |
| format | Article |
| id | doaj.art-a907e3e270bc4c15860f52e80f6ab7b5 |
| institution | Directory Open Access Journal |
| issn | 2079-6374 |
| language | English |
| last_indexed | 2024-03-11T09:05:13Z |
| publishDate | 2023-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Biosensors |
| spelling | doaj.art-a907e3e270bc4c15860f52e80f6ab7b52023-11-16T19:26:22ZengMDPI AGBiosensors2079-63742023-02-0113226110.3390/bios13020261Surface Plasmon Resonance Microscopy Based on Total Internal ReflectionTeliang Zhang0Xueliang Wang1Youjun Zeng2Songfeng Huang3Xiaoqi Dai4Weifu Kong5Qian Liu6Jiajie Chen7Junle Qu8Yonghong Shao9Key Laboratory of Optoelectronic Devices and Systems of Ministry of Education and Guangdong Province, College of Physics and Optoelectronic Engineering, Shenzhen University, Shenzhen 518060, ChinaKey Laboratory of Optoelectronic Devices and Systems of Ministry of Education and Guangdong Province, College of Physics and Optoelectronic Engineering, Shenzhen University, Shenzhen 518060, ChinaCollege of Physics and Optoelectronic Engineering, Guangdong University of Technology, Guangzhou 510006, ChinaKey Laboratory of Optoelectronic Devices and Systems of Ministry of Education and Guangdong Province, College of Physics and Optoelectronic Engineering, Shenzhen University, Shenzhen 518060, ChinaKey Laboratory of Optoelectronic Devices and Systems of Ministry of Education and Guangdong Province, College of Physics and Optoelectronic Engineering, Shenzhen University, Shenzhen 518060, ChinaKey Laboratory of Optoelectronic Devices and Systems of Ministry of Education and Guangdong Province, College of Physics and Optoelectronic Engineering, Shenzhen University, Shenzhen 518060, ChinaKey Laboratory of Optoelectronic Devices and Systems of Ministry of Education and Guangdong Province, College of Physics and Optoelectronic Engineering, Shenzhen University, Shenzhen 518060, ChinaKey Laboratory of Optoelectronic Devices and Systems of Ministry of Education and Guangdong Province, College of Physics and Optoelectronic Engineering, Shenzhen University, Shenzhen 518060, ChinaKey Laboratory of Optoelectronic Devices and Systems of Ministry of Education and Guangdong Province, College of Physics and Optoelectronic Engineering, Shenzhen University, Shenzhen 518060, ChinaKey Laboratory of Optoelectronic Devices and Systems of Ministry of Education and Guangdong Province, College of Physics and Optoelectronic Engineering, Shenzhen University, Shenzhen 518060, ChinaSurface plasmon resonance microscopy (SPRM) has been widely employed in biological fields because of its high spatial resolution and label-free detection modality. In this study, SPRM based on total internal reflection (TIR) is studied via a home-built SPRM system, and the principle of imaging of a single nanoparticle is analyzed as well. By designing a ring filter and combining it with the deconvolution algorithm in Fourier space, the parabolic tail of the nanoparticle image is removed, in which a spatial resolution of 248 nm is obtained. In addition, we also measured the specific binding between the human IgG antigen and goat anti-human IgG antibody using the TIR-based SPRM. The experimental results have proved that the system can image sparse nanoparticles and monitor biomolecular interactions.https://www.mdpi.com/2079-6374/13/2/261total internal reflectionsurface plasmon resonancesurface plasmon resonance microscopybiomolecular interaction |
| spellingShingle | Teliang Zhang Xueliang Wang Youjun Zeng Songfeng Huang Xiaoqi Dai Weifu Kong Qian Liu Jiajie Chen Junle Qu Yonghong Shao Surface Plasmon Resonance Microscopy Based on Total Internal Reflection Biosensors total internal reflection surface plasmon resonance surface plasmon resonance microscopy biomolecular interaction |
| title | Surface Plasmon Resonance Microscopy Based on Total Internal Reflection |
| title_full | Surface Plasmon Resonance Microscopy Based on Total Internal Reflection |
| title_fullStr | Surface Plasmon Resonance Microscopy Based on Total Internal Reflection |
| title_full_unstemmed | Surface Plasmon Resonance Microscopy Based on Total Internal Reflection |
| title_short | Surface Plasmon Resonance Microscopy Based on Total Internal Reflection |
| title_sort | surface plasmon resonance microscopy based on total internal reflection |
| topic | total internal reflection surface plasmon resonance surface plasmon resonance microscopy biomolecular interaction |
| url | https://www.mdpi.com/2079-6374/13/2/261 |
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