Analysis of the Antibacterial Activity and the Total Phenolic and Flavonoid Contents of the Moringa oleifera Leaf Extract as an Antimicrobial Agent against Pseudomonas aeruginosa
Pseudomonas aeruginosa is a bacterium that causes metal deterioration by forming biofilms on metal surfaces. This work was carried out to analyze the antibacterial activity and the phenolic and flavonoid contents of the Moringa oleifera leaf extract against Pseudomonas aeruginosa. M. oleifera leaves...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Hindawi Limited
2023-01-01
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| Series: | Scientifica |
| Online Access: | http://dx.doi.org/10.1155/2023/5782063 |
| _version_ | 1797663757060538368 |
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| author | Ahmad Royani Muhammad Hanafi Puspa Dewi N. Lotulung Heddy Julistiono Achmad Dinoto Azwar Manaf |
| author_facet | Ahmad Royani Muhammad Hanafi Puspa Dewi N. Lotulung Heddy Julistiono Achmad Dinoto Azwar Manaf |
| author_sort | Ahmad Royani |
| collection | DOAJ |
| description | Pseudomonas aeruginosa is a bacterium that causes metal deterioration by forming biofilms on metal surfaces. This work was carried out to analyze the antibacterial activity and the phenolic and flavonoid contents of the Moringa oleifera leaf extract against Pseudomonas aeruginosa. M. oleifera leaves were extracted in a methanol solution at different concentrations. The M. oleifera leaf extract yields were 12.84%, 18.96%, and 19.64% for the 100%, 75%, and 50% methanol ratios, respectively. Extracts of M. oleifera leaves had a minimum inhibiting concentration (MIC) of approximately 6144 μg/mL against P. aeruginosa for a ratio of 100% methanol. In addition, no antibacterial activity was found for the 75% and 50% methanol ratios. The total phenolic levels were 16.26%, 12.73%, and 12.33% for the 100%, 75%, and 50% methanol solvent ratios, respectively. The total amounts of flavonoids were 23.32%, 3.40%, and 0.64% for the 100%, 75%, and 50% methanol solvents, respectively. The chemical structure of M. oleifera consists of kaemferol-3-O-rutinoside, quercimeritrin, kaempferol-3-O-β-D-glucopyranoside, stearidonic acid, trichosanic acid, pyrophaeophorbide A, and stigmastan-3,6-dione. The concentration of the solvent is essential in the extraction of plant constituents. Different concentrations indicate differences in antibacterial activity, phenolic and flavonoid contents, and chemical structure. |
| first_indexed | 2024-03-11T19:19:21Z |
| format | Article |
| id | doaj.art-a9087cb83bbd444c9084c75c775e6668 |
| institution | Directory Open Access Journal |
| issn | 2090-908X |
| language | English |
| last_indexed | 2024-03-11T19:19:21Z |
| publishDate | 2023-01-01 |
| publisher | Hindawi Limited |
| record_format | Article |
| series | Scientifica |
| spelling | doaj.art-a9087cb83bbd444c9084c75c775e66682023-10-08T00:00:02ZengHindawi LimitedScientifica2090-908X2023-01-01202310.1155/2023/5782063Analysis of the Antibacterial Activity and the Total Phenolic and Flavonoid Contents of the Moringa oleifera Leaf Extract as an Antimicrobial Agent against Pseudomonas aeruginosaAhmad Royani0Muhammad Hanafi1Puspa Dewi N. Lotulung2Heddy Julistiono3Achmad Dinoto4Azwar Manaf5Postgraduate Program of Materials Science StudyResearch Center for Pharmaceutical Ingredients and Traditional MedicineResearch Center for Pharmaceutical Ingredients and Traditional MedicineResearch Center of Applied MicrobiologyResearch Center of Applied MicrobiologyPostgraduate Program of Materials Science StudyPseudomonas aeruginosa is a bacterium that causes metal deterioration by forming biofilms on metal surfaces. This work was carried out to analyze the antibacterial activity and the phenolic and flavonoid contents of the Moringa oleifera leaf extract against Pseudomonas aeruginosa. M. oleifera leaves were extracted in a methanol solution at different concentrations. The M. oleifera leaf extract yields were 12.84%, 18.96%, and 19.64% for the 100%, 75%, and 50% methanol ratios, respectively. Extracts of M. oleifera leaves had a minimum inhibiting concentration (MIC) of approximately 6144 μg/mL against P. aeruginosa for a ratio of 100% methanol. In addition, no antibacterial activity was found for the 75% and 50% methanol ratios. The total phenolic levels were 16.26%, 12.73%, and 12.33% for the 100%, 75%, and 50% methanol solvent ratios, respectively. The total amounts of flavonoids were 23.32%, 3.40%, and 0.64% for the 100%, 75%, and 50% methanol solvents, respectively. The chemical structure of M. oleifera consists of kaemferol-3-O-rutinoside, quercimeritrin, kaempferol-3-O-β-D-glucopyranoside, stearidonic acid, trichosanic acid, pyrophaeophorbide A, and stigmastan-3,6-dione. The concentration of the solvent is essential in the extraction of plant constituents. Different concentrations indicate differences in antibacterial activity, phenolic and flavonoid contents, and chemical structure.http://dx.doi.org/10.1155/2023/5782063 |
| spellingShingle | Ahmad Royani Muhammad Hanafi Puspa Dewi N. Lotulung Heddy Julistiono Achmad Dinoto Azwar Manaf Analysis of the Antibacterial Activity and the Total Phenolic and Flavonoid Contents of the Moringa oleifera Leaf Extract as an Antimicrobial Agent against Pseudomonas aeruginosa Scientifica |
| title | Analysis of the Antibacterial Activity and the Total Phenolic and Flavonoid Contents of the Moringa oleifera Leaf Extract as an Antimicrobial Agent against Pseudomonas aeruginosa |
| title_full | Analysis of the Antibacterial Activity and the Total Phenolic and Flavonoid Contents of the Moringa oleifera Leaf Extract as an Antimicrobial Agent against Pseudomonas aeruginosa |
| title_fullStr | Analysis of the Antibacterial Activity and the Total Phenolic and Flavonoid Contents of the Moringa oleifera Leaf Extract as an Antimicrobial Agent against Pseudomonas aeruginosa |
| title_full_unstemmed | Analysis of the Antibacterial Activity and the Total Phenolic and Flavonoid Contents of the Moringa oleifera Leaf Extract as an Antimicrobial Agent against Pseudomonas aeruginosa |
| title_short | Analysis of the Antibacterial Activity and the Total Phenolic and Flavonoid Contents of the Moringa oleifera Leaf Extract as an Antimicrobial Agent against Pseudomonas aeruginosa |
| title_sort | analysis of the antibacterial activity and the total phenolic and flavonoid contents of the moringa oleifera leaf extract as an antimicrobial agent against pseudomonas aeruginosa |
| url | http://dx.doi.org/10.1155/2023/5782063 |
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