Pigment Cell Differentiation in Sea Urchin Blastula-Derived Primary Cell Cultures
The quinone pigments of sea urchins, specifically echinochrome and spinochromes, are known for their effective antioxidant, antibacterial, antifungal, and antitumor activities. We developed in vitro technology for inducing pigment differentiation in cell culture. The intensification of the pigment d...
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| Format: | Article |
| Language: | English |
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MDPI AG
2014-06-01
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| Series: | Marine Drugs |
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| Online Access: | http://www.mdpi.com/1660-3397/12/7/3874 |
| _version_ | 1798006757113462784 |
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| author | Natalya V. Ageenko Konstantin V. Kiselev Pavel S. Dmitrenok Nelly A. Odintsova |
| author_facet | Natalya V. Ageenko Konstantin V. Kiselev Pavel S. Dmitrenok Nelly A. Odintsova |
| author_sort | Natalya V. Ageenko |
| collection | DOAJ |
| description | The quinone pigments of sea urchins, specifically echinochrome and spinochromes, are known for their effective antioxidant, antibacterial, antifungal, and antitumor activities. We developed in vitro technology for inducing pigment differentiation in cell culture. The intensification of the pigment differentiation was accompanied by a simultaneous decrease in cell proliferation. The number of pigment cells was two-fold higher in the cells cultivated in the coelomic fluids of injured sea urchins than in those intact. The possible roles of the specific components of the coelomic fluids in the pigment differentiation process and the quantitative measurement of the production of naphthoquinone pigments during cultivation were examined by MALDI and electrospray ionization mass spectrometry. Echinochrome A and spinochrome E were produced by the cultivated cells of the sand dollar Scaphechinus mirabilis in all tested media, while only spinochromes were found in the cultivated cells of another sea urchin, Strongylocentrotus intermedius. The expression of genes associated with the induction of pigment differentiation was increased in cells cultivated in the presence of shikimic acid, a precursor of naphthoquinone pigments. Our results should contribute to the development of new techniques in marine biotechnology, including the generation of cell cultures producing complex bioactive compounds with therapeutic potential. |
| first_indexed | 2024-04-11T13:00:45Z |
| format | Article |
| id | doaj.art-a90dc1394720415cb384d1afee3d0d0e |
| institution | Directory Open Access Journal |
| issn | 1660-3397 |
| language | English |
| last_indexed | 2024-04-11T13:00:45Z |
| publishDate | 2014-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Marine Drugs |
| spelling | doaj.art-a90dc1394720415cb384d1afee3d0d0e2022-12-22T04:22:58ZengMDPI AGMarine Drugs1660-33972014-06-011273874389110.3390/md12073874md12073874Pigment Cell Differentiation in Sea Urchin Blastula-Derived Primary Cell CulturesNatalya V. Ageenko0Konstantin V. Kiselev1Pavel S. Dmitrenok2Nelly A. Odintsova3Cytotechnology Laboratory, A.V. Zhirmunsky Institute of Marine Biology, FEB RAS, Vladivostok 690041, RussiaLaboratory of Biotechnology, Institute of Biology and Soil Sciences, FEB RAS, Vladivostok 690022, RussiaLaboratory of Instrumental and Radioisotope Methods of Analysis, G.B. Elyakov Pacific Institute of Bioorganic Chemistry, FEB RAS, Vladivostok 690022, RussiaCytotechnology Laboratory, A.V. Zhirmunsky Institute of Marine Biology, FEB RAS, Vladivostok 690041, RussiaThe quinone pigments of sea urchins, specifically echinochrome and spinochromes, are known for their effective antioxidant, antibacterial, antifungal, and antitumor activities. We developed in vitro technology for inducing pigment differentiation in cell culture. The intensification of the pigment differentiation was accompanied by a simultaneous decrease in cell proliferation. The number of pigment cells was two-fold higher in the cells cultivated in the coelomic fluids of injured sea urchins than in those intact. The possible roles of the specific components of the coelomic fluids in the pigment differentiation process and the quantitative measurement of the production of naphthoquinone pigments during cultivation were examined by MALDI and electrospray ionization mass spectrometry. Echinochrome A and spinochrome E were produced by the cultivated cells of the sand dollar Scaphechinus mirabilis in all tested media, while only spinochromes were found in the cultivated cells of another sea urchin, Strongylocentrotus intermedius. The expression of genes associated with the induction of pigment differentiation was increased in cells cultivated in the presence of shikimic acid, a precursor of naphthoquinone pigments. Our results should contribute to the development of new techniques in marine biotechnology, including the generation of cell cultures producing complex bioactive compounds with therapeutic potential.http://www.mdpi.com/1660-3397/12/7/3874cell cultureechinochromegene expressionMALDI TOF MSESI MSmarine biotechnologynaphthoquinone pigmentspigment differentiationproliferationsea urchin |
| spellingShingle | Natalya V. Ageenko Konstantin V. Kiselev Pavel S. Dmitrenok Nelly A. Odintsova Pigment Cell Differentiation in Sea Urchin Blastula-Derived Primary Cell Cultures Marine Drugs cell culture echinochrome gene expression MALDI TOF MS ESI MS marine biotechnology naphthoquinone pigments pigment differentiation proliferation sea urchin |
| title | Pigment Cell Differentiation in Sea Urchin Blastula-Derived Primary Cell Cultures |
| title_full | Pigment Cell Differentiation in Sea Urchin Blastula-Derived Primary Cell Cultures |
| title_fullStr | Pigment Cell Differentiation in Sea Urchin Blastula-Derived Primary Cell Cultures |
| title_full_unstemmed | Pigment Cell Differentiation in Sea Urchin Blastula-Derived Primary Cell Cultures |
| title_short | Pigment Cell Differentiation in Sea Urchin Blastula-Derived Primary Cell Cultures |
| title_sort | pigment cell differentiation in sea urchin blastula derived primary cell cultures |
| topic | cell culture echinochrome gene expression MALDI TOF MS ESI MS marine biotechnology naphthoquinone pigments pigment differentiation proliferation sea urchin |
| url | http://www.mdpi.com/1660-3397/12/7/3874 |
| work_keys_str_mv | AT natalyavageenko pigmentcelldifferentiationinseaurchinblastuladerivedprimarycellcultures AT konstantinvkiselev pigmentcelldifferentiationinseaurchinblastuladerivedprimarycellcultures AT pavelsdmitrenok pigmentcelldifferentiationinseaurchinblastuladerivedprimarycellcultures AT nellyaodintsova pigmentcelldifferentiationinseaurchinblastuladerivedprimarycellcultures |