Expression Profile, Regulatory Network, and Putative Role of microRNAs in the Developmental Process of Asian Honey Bee Larval Guts
MiRNAs, as a kind of key regulators in gene expression, play vital roles in numerous life activities from cellular proliferation and differentiation to development and immunity. However, little is known about the regulatory manner of miRNAs in the development of Asian honey bee (<i>Apis cerana...
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-05-01
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| Series: | Insects |
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| Online Access: | https://www.mdpi.com/2075-4450/14/5/469 |
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| author | Xiaoxue Fan Wende Zhang Sijia Guo Leran Zhu Yiqiong Zhang Haodong Zhao Xuze Gao Haibin Jiang Tianze Zhang Dafu Chen Rui Guo Qingsheng Niu |
| author_facet | Xiaoxue Fan Wende Zhang Sijia Guo Leran Zhu Yiqiong Zhang Haodong Zhao Xuze Gao Haibin Jiang Tianze Zhang Dafu Chen Rui Guo Qingsheng Niu |
| author_sort | Xiaoxue Fan |
| collection | DOAJ |
| description | MiRNAs, as a kind of key regulators in gene expression, play vital roles in numerous life activities from cellular proliferation and differentiation to development and immunity. However, little is known about the regulatory manner of miRNAs in the development of Asian honey bee (<i>Apis cerana</i>) guts. Here, on basis of our previously gained high-quality transcriptome data, transcriptome-wide identification of miRNAs in the larval guts of <i>Apis cerana cerana</i> was conducted, followed by investigation of the miRNAs’ differential expression profile during the gut development. In addition to the regulatory network, the potential function of differentially expressed miRNAs (DEmiRNAs) was further analyzed. In total, 330, 351, and 321 miRNAs were identified in the 4-, 5-, and 6-day-old larval guts, respectively; among these, 257 miRNAs were shared, while 38, 51, and 36 ones were specifically expressed. Sequences of six miRNAs were confirmed by stem-loop RT-PCR and Sanger sequencing. Additionally, in the “Ac4 vs. Ac5” comparison group, there were seven up-regulated and eight down-regulated miRNAs; these DEmiRNAs could target 5041 mRNAs, involving a series of GO terms and KEGG pathways associated with growth and development, such as cellular process, cell part, Wnt, and Hippo. Comparatively, four up-regulated and six down-regulated miRNAs detected in the “Ac5 vs. Ac6” comparison group and the targets were associated with diverse development-related terms and pathways, including cell, organelle, Notch and Wnt. Intriguingly, it was noticed that miR-6001-y presented a continuous up-regulation trend across the developmental process of larval guts, implying that miR-6001-y may be a potential essential modulator in the development process of larval guts. Further investigation indicated that 43 targets in the “Ac4 vs. Ac5” comparison group and 31 targets in the “Ac5 vs. Ac6” comparison group were engaged in several crucial development-associated signaling pathways such as Wnt, Hippo, and Notch. Ultimately, the expression trends of five randomly selected DEmiRNAs were verified using RT-qPCR. These results demonstrated that dynamic expression and structural alteration of miRNAs were accompanied by the development of <i>A. c. cerana</i> larval guts, and DEmiRNAs were likely to participate in the modulation of growth as well as development of larval guts by affecting several critical pathways via regulation of the expression of target genes. Our data offer a basis for elucidating the developmental mechanism underlying Asian honey bee larval guts. |
| first_indexed | 2024-03-11T03:37:45Z |
| format | Article |
| id | doaj.art-a90df97b16694ceba0eddd8b215328f1 |
| institution | Directory Open Access Journal |
| issn | 2075-4450 |
| language | English |
| last_indexed | 2024-03-11T03:37:45Z |
| publishDate | 2023-05-01 |
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| record_format | Article |
| series | Insects |
| spelling | doaj.art-a90df97b16694ceba0eddd8b215328f12023-11-18T01:50:26ZengMDPI AGInsects2075-44502023-05-0114546910.3390/insects14050469Expression Profile, Regulatory Network, and Putative Role of microRNAs in the Developmental Process of Asian Honey Bee Larval GutsXiaoxue Fan0Wende Zhang1Sijia Guo2Leran Zhu3Yiqiong Zhang4Haodong Zhao5Xuze Gao6Haibin Jiang7Tianze Zhang8Dafu Chen9Rui Guo10Qingsheng Niu11College of Animal Sciences (College of Bee Science), Fujian Agriculture and Forestry University, Fuzhou 350002, ChinaCollege of Animal Sciences (College of Bee Science), Fujian Agriculture and Forestry University, Fuzhou 350002, ChinaCollege of Animal Sciences (College of Bee Science), Fujian Agriculture and Forestry University, Fuzhou 350002, ChinaCollege of Animal Sciences (College of Bee Science), Fujian Agriculture and Forestry University, Fuzhou 350002, ChinaCollege of Animal Sciences (College of Bee Science), Fujian Agriculture and Forestry University, Fuzhou 350002, ChinaCollege of Animal Sciences (College of Bee Science), Fujian Agriculture and Forestry University, Fuzhou 350002, ChinaCollege of Animal Sciences (College of Bee Science), Fujian Agriculture and Forestry University, Fuzhou 350002, ChinaApiculture Science Institute of Jilin Province, Jilin 132000, ChinaCollege of Animal Sciences (College of Bee Science), Fujian Agriculture and Forestry University, Fuzhou 350002, ChinaCollege of Animal Sciences (College of Bee Science), Fujian Agriculture and Forestry University, Fuzhou 350002, ChinaCollege of Animal Sciences (College of Bee Science), Fujian Agriculture and Forestry University, Fuzhou 350002, ChinaApiculture Science Institute of Jilin Province, Jilin 132000, ChinaMiRNAs, as a kind of key regulators in gene expression, play vital roles in numerous life activities from cellular proliferation and differentiation to development and immunity. However, little is known about the regulatory manner of miRNAs in the development of Asian honey bee (<i>Apis cerana</i>) guts. Here, on basis of our previously gained high-quality transcriptome data, transcriptome-wide identification of miRNAs in the larval guts of <i>Apis cerana cerana</i> was conducted, followed by investigation of the miRNAs’ differential expression profile during the gut development. In addition to the regulatory network, the potential function of differentially expressed miRNAs (DEmiRNAs) was further analyzed. In total, 330, 351, and 321 miRNAs were identified in the 4-, 5-, and 6-day-old larval guts, respectively; among these, 257 miRNAs were shared, while 38, 51, and 36 ones were specifically expressed. Sequences of six miRNAs were confirmed by stem-loop RT-PCR and Sanger sequencing. Additionally, in the “Ac4 vs. Ac5” comparison group, there were seven up-regulated and eight down-regulated miRNAs; these DEmiRNAs could target 5041 mRNAs, involving a series of GO terms and KEGG pathways associated with growth and development, such as cellular process, cell part, Wnt, and Hippo. Comparatively, four up-regulated and six down-regulated miRNAs detected in the “Ac5 vs. Ac6” comparison group and the targets were associated with diverse development-related terms and pathways, including cell, organelle, Notch and Wnt. Intriguingly, it was noticed that miR-6001-y presented a continuous up-regulation trend across the developmental process of larval guts, implying that miR-6001-y may be a potential essential modulator in the development process of larval guts. Further investigation indicated that 43 targets in the “Ac4 vs. Ac5” comparison group and 31 targets in the “Ac5 vs. Ac6” comparison group were engaged in several crucial development-associated signaling pathways such as Wnt, Hippo, and Notch. Ultimately, the expression trends of five randomly selected DEmiRNAs were verified using RT-qPCR. These results demonstrated that dynamic expression and structural alteration of miRNAs were accompanied by the development of <i>A. c. cerana</i> larval guts, and DEmiRNAs were likely to participate in the modulation of growth as well as development of larval guts by affecting several critical pathways via regulation of the expression of target genes. Our data offer a basis for elucidating the developmental mechanism underlying Asian honey bee larval guts.https://www.mdpi.com/2075-4450/14/5/469honey bee<i>Apis cerana cerana</i>miRNAregulatory networklarvagut |
| spellingShingle | Xiaoxue Fan Wende Zhang Sijia Guo Leran Zhu Yiqiong Zhang Haodong Zhao Xuze Gao Haibin Jiang Tianze Zhang Dafu Chen Rui Guo Qingsheng Niu Expression Profile, Regulatory Network, and Putative Role of microRNAs in the Developmental Process of Asian Honey Bee Larval Guts Insects honey bee <i>Apis cerana cerana</i> miRNA regulatory network larva gut |
| title | Expression Profile, Regulatory Network, and Putative Role of microRNAs in the Developmental Process of Asian Honey Bee Larval Guts |
| title_full | Expression Profile, Regulatory Network, and Putative Role of microRNAs in the Developmental Process of Asian Honey Bee Larval Guts |
| title_fullStr | Expression Profile, Regulatory Network, and Putative Role of microRNAs in the Developmental Process of Asian Honey Bee Larval Guts |
| title_full_unstemmed | Expression Profile, Regulatory Network, and Putative Role of microRNAs in the Developmental Process of Asian Honey Bee Larval Guts |
| title_short | Expression Profile, Regulatory Network, and Putative Role of microRNAs in the Developmental Process of Asian Honey Bee Larval Guts |
| title_sort | expression profile regulatory network and putative role of micrornas in the developmental process of asian honey bee larval guts |
| topic | honey bee <i>Apis cerana cerana</i> miRNA regulatory network larva gut |
| url | https://www.mdpi.com/2075-4450/14/5/469 |
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