Dysregulation of Plasma miR-146a and miR-155 Expression Profile in Mycosis Fungoides Is Associated with rs2910164 and rs767649 Polymorphisms
Diagnosis of Mycosis Fungoides (MF) may be challenging, due to its polymorphic nature. The use of miRNAs as biomarkers to assist in diagnosis has been investigated, mainly in skin lesion biopsies. The purpose of this study is to evaluate the plasma levels of miR-146a and miR-155 in MF patients and t...
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MDPI AG
2022-12-01
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| Series: | International Journal of Molecular Sciences |
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| author | Chrysostomos Avgeros Aikaterini Patsatsi Dimitrios Dimitriadis Andigoni Malousi Triantafyllia Koletsa Despoina Papathemeli Antonia Syrnioti Paraskevi Avgerou Elizabeth Lazaridou Georgios Tzimagiorgis Elisavet Georgiou |
| author_facet | Chrysostomos Avgeros Aikaterini Patsatsi Dimitrios Dimitriadis Andigoni Malousi Triantafyllia Koletsa Despoina Papathemeli Antonia Syrnioti Paraskevi Avgerou Elizabeth Lazaridou Georgios Tzimagiorgis Elisavet Georgiou |
| author_sort | Chrysostomos Avgeros |
| collection | DOAJ |
| description | Diagnosis of Mycosis Fungoides (MF) may be challenging, due to its polymorphic nature. The use of miRNAs as biomarkers to assist in diagnosis has been investigated, mainly in skin lesion biopsies. The purpose of this study is to evaluate the plasma levels of miR-146a and miR-155 in MF patients and to investigate their association with SNPs of their genes. Plasma miRNAs were quantified by RT-qPCR. Genomic DNA was used for SNPs’ genotyping by Sanger sequencing. Plasma levels of miR-146a and miR-155 were significantly higher in patients vs. controls, in early MF patients vs. controls, and in advanced vs. early MF patients. Both miRNAs’ levels were significantly higher in stage IIB vs. early-stage patients. miR-155 plasma levels were significantly higher in patients with skin tumors or erythroderma. CC genotype (rs2910164 C>G) was significantly more frequent in healthy controls and associated with lower MF risk and lower miR-146a levels. The AA genotype (rs767649 T>A) was significantly more frequent in patients and correlated with increased MF risk and increased miR-155 levels. The combination of GG+AA was only detected in patients and was correlated with higher MF susceptibility. Increased mir-146a and mir-155 plasma levels in MF is an important finding to establish putative noninvasive biomarkers. The presence of SNPs is closely associated with miRs’ expression, and possibly with disease susceptibility. |
| first_indexed | 2024-03-11T10:00:07Z |
| format | Article |
| id | doaj.art-a90ebca20216432789616a50c9018569 |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-11T10:00:07Z |
| publishDate | 2022-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-a90ebca20216432789616a50c90185692023-11-16T15:31:00ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672022-12-0124127110.3390/ijms24010271Dysregulation of Plasma miR-146a and miR-155 Expression Profile in Mycosis Fungoides Is Associated with rs2910164 and rs767649 PolymorphismsChrysostomos Avgeros0Aikaterini Patsatsi1Dimitrios Dimitriadis2Andigoni Malousi3Triantafyllia Koletsa4Despoina Papathemeli5Antonia Syrnioti6Paraskevi Avgerou7Elizabeth Lazaridou8Georgios Tzimagiorgis9Elisavet Georgiou10Laboratory of Biological Chemistry, School of Medicine, Faculty of Health Sciences, Aristotle University of Thessaloniki, 54124 Thessaloniki, Greece2nd Dermatology Department, School of Medicine, Faculty of Health Sciences, Aristotle University of Thessaloniki, “Papageorgiou” General Hospital, 56403 Thessaloniki, GreeceSchool of Economics, Aristotle University of Thessaloniki, 54124 Thessaloniki, GreeceLaboratory of Biological Chemistry, School of Medicine, Faculty of Health Sciences, Aristotle University of Thessaloniki, 54124 Thessaloniki, GreeceDepartment of Pathology, School of Medicine, Faculty of Health Sciences, Aristotle University of Thessaloniki, 54124 Thessaloniki, Greece2nd Dermatology Department, School of Medicine, Faculty of Health Sciences, Aristotle University of Thessaloniki, “Papageorgiou” General Hospital, 56403 Thessaloniki, GreeceDepartment of Pathology, School of Medicine, Faculty of Health Sciences, Aristotle University of Thessaloniki, 54124 Thessaloniki, GreeceLaboratory of Biological Chemistry, School of Medicine, Faculty of Health Sciences, Aristotle University of Thessaloniki, 54124 Thessaloniki, Greece2nd Dermatology Department, School of Medicine, Faculty of Health Sciences, Aristotle University of Thessaloniki, “Papageorgiou” General Hospital, 56403 Thessaloniki, GreeceLaboratory of Biological Chemistry, School of Medicine, Faculty of Health Sciences, Aristotle University of Thessaloniki, 54124 Thessaloniki, GreeceLaboratory of Biological Chemistry, School of Medicine, Faculty of Health Sciences, Aristotle University of Thessaloniki, 54124 Thessaloniki, GreeceDiagnosis of Mycosis Fungoides (MF) may be challenging, due to its polymorphic nature. The use of miRNAs as biomarkers to assist in diagnosis has been investigated, mainly in skin lesion biopsies. The purpose of this study is to evaluate the plasma levels of miR-146a and miR-155 in MF patients and to investigate their association with SNPs of their genes. Plasma miRNAs were quantified by RT-qPCR. Genomic DNA was used for SNPs’ genotyping by Sanger sequencing. Plasma levels of miR-146a and miR-155 were significantly higher in patients vs. controls, in early MF patients vs. controls, and in advanced vs. early MF patients. Both miRNAs’ levels were significantly higher in stage IIB vs. early-stage patients. miR-155 plasma levels were significantly higher in patients with skin tumors or erythroderma. CC genotype (rs2910164 C>G) was significantly more frequent in healthy controls and associated with lower MF risk and lower miR-146a levels. The AA genotype (rs767649 T>A) was significantly more frequent in patients and correlated with increased MF risk and increased miR-155 levels. The combination of GG+AA was only detected in patients and was correlated with higher MF susceptibility. Increased mir-146a and mir-155 plasma levels in MF is an important finding to establish putative noninvasive biomarkers. The presence of SNPs is closely associated with miRs’ expression, and possibly with disease susceptibility.https://www.mdpi.com/1422-0067/24/1/271miRNAsmycosis fungoidessingle-nucleotide polymorphismCTCL |
| spellingShingle | Chrysostomos Avgeros Aikaterini Patsatsi Dimitrios Dimitriadis Andigoni Malousi Triantafyllia Koletsa Despoina Papathemeli Antonia Syrnioti Paraskevi Avgerou Elizabeth Lazaridou Georgios Tzimagiorgis Elisavet Georgiou Dysregulation of Plasma miR-146a and miR-155 Expression Profile in Mycosis Fungoides Is Associated with rs2910164 and rs767649 Polymorphisms International Journal of Molecular Sciences miRNAs mycosis fungoides single-nucleotide polymorphism CTCL |
| title | Dysregulation of Plasma miR-146a and miR-155 Expression Profile in Mycosis Fungoides Is Associated with rs2910164 and rs767649 Polymorphisms |
| title_full | Dysregulation of Plasma miR-146a and miR-155 Expression Profile in Mycosis Fungoides Is Associated with rs2910164 and rs767649 Polymorphisms |
| title_fullStr | Dysregulation of Plasma miR-146a and miR-155 Expression Profile in Mycosis Fungoides Is Associated with rs2910164 and rs767649 Polymorphisms |
| title_full_unstemmed | Dysregulation of Plasma miR-146a and miR-155 Expression Profile in Mycosis Fungoides Is Associated with rs2910164 and rs767649 Polymorphisms |
| title_short | Dysregulation of Plasma miR-146a and miR-155 Expression Profile in Mycosis Fungoides Is Associated with rs2910164 and rs767649 Polymorphisms |
| title_sort | dysregulation of plasma mir 146a and mir 155 expression profile in mycosis fungoides is associated with rs2910164 and rs767649 polymorphisms |
| topic | miRNAs mycosis fungoides single-nucleotide polymorphism CTCL |
| url | https://www.mdpi.com/1422-0067/24/1/271 |
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