Identification and characterization of resistance to cowpea aphid (<it>Aphis craccivora</it> Koch) in <it>Medicago truncatula</it>
<p>Abstract</p> <p>Background</p> <p>Cowpea aphid (CPA; <it>Aphis craccivora</it>) is the most important insect pest of cowpea and also causes significant yield losses in other legume crops including alfalfa, beans, chickpea, lentils, lupins and peanuts. In...
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| Format: | Article |
| Language: | English |
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BMC
2012-07-01
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| Series: | BMC Plant Biology |
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| Online Access: | http://www.biomedcentral.com/1471-2229/12/101 |
| _version_ | 1819087060946911232 |
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| author | Kamphuis Lars G Gao Lingling Singh Karam B |
| author_facet | Kamphuis Lars G Gao Lingling Singh Karam B |
| author_sort | Kamphuis Lars G |
| collection | DOAJ |
| description | <p>Abstract</p> <p>Background</p> <p>Cowpea aphid (CPA; <it>Aphis craccivora</it>) is the most important insect pest of cowpea and also causes significant yield losses in other legume crops including alfalfa, beans, chickpea, lentils, lupins and peanuts. In many of these crops there is no natural genetic resistance to this sap-sucking insect or resistance genes have been overcome by newly emerged CPA biotypes.</p> <p>Results</p> <p>In this study, we screened a subset of the <it>Medicago truncatula</it> core collection of the South Australian Research and Development Institute (SARDI) and identified strong resistance to CPA in a <it>M. truncatula</it> accession SA30199, compared to all other <it>M. truncatula</it> accessions tested. The biology of resistance to CPA in SA30199 plants was characterised compared to the highly susceptible accession Borung and showed that resistance occurred at the level of the phloem, required an intact plant and involved a combination of antixenosis and antibiosis. Quantitative trait loci (QTL) analysis using a F<sub>2</sub> population (n = 150) from a cross between SA30199 and Borung revealed that resistance to CPA is controlled in part by a major quantitative trait locus (QTL) on chromosome 2, explaining 39% of the antibiosis resistance.</p> <p>Conclusions</p> <p>The identification of strong CPA resistance in <it>M. truncatula</it> allows for the identification of key regulators and genes important in this model legume to give effective CPA resistance that may have relevance for other legume crops. The identified locus will also facilitate marker assisted breeding of <it>M. truncatula</it> for increased resistance to CPA and potentially other closely related Medicago species such as alfalfa.</p> |
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| institution | Directory Open Access Journal |
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| language | English |
| last_indexed | 2024-12-21T21:30:09Z |
| publishDate | 2012-07-01 |
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| series | BMC Plant Biology |
| spelling | doaj.art-a9107d3667d84c9ba319ff59ad537cc32022-12-21T18:49:38ZengBMCBMC Plant Biology1471-22292012-07-0112110110.1186/1471-2229-12-101Identification and characterization of resistance to cowpea aphid (<it>Aphis craccivora</it> Koch) in <it>Medicago truncatula</it>Kamphuis Lars GGao LinglingSingh Karam B<p>Abstract</p> <p>Background</p> <p>Cowpea aphid (CPA; <it>Aphis craccivora</it>) is the most important insect pest of cowpea and also causes significant yield losses in other legume crops including alfalfa, beans, chickpea, lentils, lupins and peanuts. In many of these crops there is no natural genetic resistance to this sap-sucking insect or resistance genes have been overcome by newly emerged CPA biotypes.</p> <p>Results</p> <p>In this study, we screened a subset of the <it>Medicago truncatula</it> core collection of the South Australian Research and Development Institute (SARDI) and identified strong resistance to CPA in a <it>M. truncatula</it> accession SA30199, compared to all other <it>M. truncatula</it> accessions tested. The biology of resistance to CPA in SA30199 plants was characterised compared to the highly susceptible accession Borung and showed that resistance occurred at the level of the phloem, required an intact plant and involved a combination of antixenosis and antibiosis. Quantitative trait loci (QTL) analysis using a F<sub>2</sub> population (n = 150) from a cross between SA30199 and Borung revealed that resistance to CPA is controlled in part by a major quantitative trait locus (QTL) on chromosome 2, explaining 39% of the antibiosis resistance.</p> <p>Conclusions</p> <p>The identification of strong CPA resistance in <it>M. truncatula</it> allows for the identification of key regulators and genes important in this model legume to give effective CPA resistance that may have relevance for other legume crops. The identified locus will also facilitate marker assisted breeding of <it>M. truncatula</it> for increased resistance to CPA and potentially other closely related Medicago species such as alfalfa.</p>http://www.biomedcentral.com/1471-2229/12/101AntibiosisAntixenosisEPGHerbivorySap-sucking insectPhloem |
| spellingShingle | Kamphuis Lars G Gao Lingling Singh Karam B Identification and characterization of resistance to cowpea aphid (<it>Aphis craccivora</it> Koch) in <it>Medicago truncatula</it> BMC Plant Biology Antibiosis Antixenosis EPG Herbivory Sap-sucking insect Phloem |
| title | Identification and characterization of resistance to cowpea aphid (<it>Aphis craccivora</it> Koch) in <it>Medicago truncatula</it> |
| title_full | Identification and characterization of resistance to cowpea aphid (<it>Aphis craccivora</it> Koch) in <it>Medicago truncatula</it> |
| title_fullStr | Identification and characterization of resistance to cowpea aphid (<it>Aphis craccivora</it> Koch) in <it>Medicago truncatula</it> |
| title_full_unstemmed | Identification and characterization of resistance to cowpea aphid (<it>Aphis craccivora</it> Koch) in <it>Medicago truncatula</it> |
| title_short | Identification and characterization of resistance to cowpea aphid (<it>Aphis craccivora</it> Koch) in <it>Medicago truncatula</it> |
| title_sort | identification and characterization of resistance to cowpea aphid it aphis craccivora it koch in it medicago truncatula it |
| topic | Antibiosis Antixenosis EPG Herbivory Sap-sucking insect Phloem |
| url | http://www.biomedcentral.com/1471-2229/12/101 |
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