Neuro-Protection and Neuro-Therapy Effects of Acalypha indica Linn. Water Extract Ex Vivo on Musculus gastrocnemius Frog
The studies of neuro-protection and neuro-therapy effects of Acalypha indica Linn. water extract ex vivo on Musculus gastrocnemius frog have already done at three Departments in Faculty of Medicine, University of Indonesia. The experimental studies were done on 2 groups of frog for neuro-protection...
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Universitas Indonesia
2010-10-01
|
| Series: | Makara Journal of Health Research |
| Subjects: | |
| Online Access: | http://journal.ui.ac.id/index.php/health/article/view/302 |
| _version_ | 1797728845508378624 |
|---|---|
| author | Ernie Hernawati Purwaningsih Nurhadi Ibrahim Hamdani Zain Arjo Tedjo |
| author_facet | Ernie Hernawati Purwaningsih Nurhadi Ibrahim Hamdani Zain Arjo Tedjo |
| author_sort | Ernie Hernawati Purwaningsih |
| collection | DOAJ |
| description | The studies of neuro-protection and neuro-therapy effects of Acalypha indica Linn. water extract ex vivo on Musculus gastrocnemius frog have already done at three Departments in Faculty of Medicine, University of Indonesia. The experimental studies were done on 2 groups of frog for neuro-protection and neuro-therapy effects. Each group of frog was divided into 7 subgroups of application, 4 samples each. There were 5 subgroups of doses: 5; 10; 15; 20; 25 mg and 2 subgroups as control. Pancuronium bromide 0.2%, 4 mg, was used for a positive control as muscle relaxant. Neuro-protection study was done as follow: ringer - extract - pancuronium bromide, and neuro-therapy study was ringer - pancuronium bromide - extract, respectively. The parameters measured in these studies were the electrical activities such as amount and duration (second) of re-polarization; depolarization, resting potential, and the height of spike after electrical stimulation at 5 mV. Neuro-protection effect of extract was determined by the ability of muscle to show the electrical response after incubating with pancuronium bromide for 10 minutes, and after incubating with extract for 10 minutes for neuro-therapy effect. In the dose of 15 mg and 20 mg/mL of A. indica Linn. extract showed better activities than the dose of 25 mg of extract, both as neuro-protection and neuro-therapy effects, but statistically its have not a significant difference. This study should be followed by an in vivo experiment on frog and it would be done in pharmacokinetic and pharmacodynamic studies on other animal models. |
| first_indexed | 2024-03-12T11:19:58Z |
| format | Article |
| id | doaj.art-a913c7f371c041efae37adf229c23c68 |
| institution | Directory Open Access Journal |
| issn | 2356-3664 2356-3656 |
| language | English |
| last_indexed | 2024-03-12T11:19:58Z |
| publishDate | 2010-10-01 |
| publisher | Universitas Indonesia |
| record_format | Article |
| series | Makara Journal of Health Research |
| spelling | doaj.art-a913c7f371c041efae37adf229c23c682023-09-02T00:47:00ZengUniversitas IndonesiaMakara Journal of Health Research2356-36642356-36562010-10-01122707510.7454/msk.v12i2.302294Neuro-Protection and Neuro-Therapy Effects of Acalypha indica Linn. Water Extract Ex Vivo on Musculus gastrocnemius FrogErnie Hernawati Purwaningsih0Nurhadi Ibrahim1Hamdani Zain2Arjo Tedjo3Department of Medical Pharmacy, Faculty of Medicine, University of Indonesia, Jakarta 10430Department of Physiology, Faculty of Medicine, University of Indonesia, Jakarta 10430,Department of Medical Physic, Faculty of Medicine, University of Indonesia, Jakarta 10430,Department of Medical Chemistry, Faculty of Medicine, University of Indonesia, Jakarta 10430The studies of neuro-protection and neuro-therapy effects of Acalypha indica Linn. water extract ex vivo on Musculus gastrocnemius frog have already done at three Departments in Faculty of Medicine, University of Indonesia. The experimental studies were done on 2 groups of frog for neuro-protection and neuro-therapy effects. Each group of frog was divided into 7 subgroups of application, 4 samples each. There were 5 subgroups of doses: 5; 10; 15; 20; 25 mg and 2 subgroups as control. Pancuronium bromide 0.2%, 4 mg, was used for a positive control as muscle relaxant. Neuro-protection study was done as follow: ringer - extract - pancuronium bromide, and neuro-therapy study was ringer - pancuronium bromide - extract, respectively. The parameters measured in these studies were the electrical activities such as amount and duration (second) of re-polarization; depolarization, resting potential, and the height of spike after electrical stimulation at 5 mV. Neuro-protection effect of extract was determined by the ability of muscle to show the electrical response after incubating with pancuronium bromide for 10 minutes, and after incubating with extract for 10 minutes for neuro-therapy effect. In the dose of 15 mg and 20 mg/mL of A. indica Linn. extract showed better activities than the dose of 25 mg of extract, both as neuro-protection and neuro-therapy effects, but statistically its have not a significant difference. This study should be followed by an in vivo experiment on frog and it would be done in pharmacokinetic and pharmacodynamic studies on other animal models.http://journal.ui.ac.id/index.php/health/article/view/302Acalypha indica Linn. ex vivoneuro-protectionneuro-therapy |
| spellingShingle | Ernie Hernawati Purwaningsih Nurhadi Ibrahim Hamdani Zain Arjo Tedjo Neuro-Protection and Neuro-Therapy Effects of Acalypha indica Linn. Water Extract Ex Vivo on Musculus gastrocnemius Frog Makara Journal of Health Research Acalypha indica Linn. ex vivo neuro-protection neuro-therapy |
| title | Neuro-Protection and Neuro-Therapy Effects of Acalypha indica Linn. Water Extract Ex Vivo on Musculus gastrocnemius Frog |
| title_full | Neuro-Protection and Neuro-Therapy Effects of Acalypha indica Linn. Water Extract Ex Vivo on Musculus gastrocnemius Frog |
| title_fullStr | Neuro-Protection and Neuro-Therapy Effects of Acalypha indica Linn. Water Extract Ex Vivo on Musculus gastrocnemius Frog |
| title_full_unstemmed | Neuro-Protection and Neuro-Therapy Effects of Acalypha indica Linn. Water Extract Ex Vivo on Musculus gastrocnemius Frog |
| title_short | Neuro-Protection and Neuro-Therapy Effects of Acalypha indica Linn. Water Extract Ex Vivo on Musculus gastrocnemius Frog |
| title_sort | neuro protection and neuro therapy effects of acalypha indica linn water extract ex vivo on musculus gastrocnemius frog |
| topic | Acalypha indica Linn. ex vivo neuro-protection neuro-therapy |
| url | http://journal.ui.ac.id/index.php/health/article/view/302 |
| work_keys_str_mv | AT erniehernawatipurwaningsih neuroprotectionandneurotherapyeffectsofacalyphaindicalinnwaterextractexvivoonmusculusgastrocnemiusfrog AT nurhadiibrahim neuroprotectionandneurotherapyeffectsofacalyphaindicalinnwaterextractexvivoonmusculusgastrocnemiusfrog AT hamdanizain neuroprotectionandneurotherapyeffectsofacalyphaindicalinnwaterextractexvivoonmusculusgastrocnemiusfrog AT arjotedjo neuroprotectionandneurotherapyeffectsofacalyphaindicalinnwaterextractexvivoonmusculusgastrocnemiusfrog |