E-Cadherin Expression Distinguishes Mouse from Human Hematopoiesis in the Basophil and Erythroid Lineages
E-cadherin is a key regulator of epithelial cell–cell adhesion, the loss of which accelerates tumor growth and invasion. E-cadherin is also expressed in hematopoietic cells as well as epithelia. The function of hematopoietic E-cadherin is, however, mostly elusive. In this study, we explored the vali...
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-11-01
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| Series: | Biomolecules |
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| Online Access: | https://www.mdpi.com/2218-273X/12/11/1706 |
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| author | Rosa A. Krimpenfort Felix M. Behr Marja Nieuwland Iris de Rink Ron Kerkhoven Marieke von Lindern Micha Nethe |
| author_facet | Rosa A. Krimpenfort Felix M. Behr Marja Nieuwland Iris de Rink Ron Kerkhoven Marieke von Lindern Micha Nethe |
| author_sort | Rosa A. Krimpenfort |
| collection | DOAJ |
| description | E-cadherin is a key regulator of epithelial cell–cell adhesion, the loss of which accelerates tumor growth and invasion. E-cadherin is also expressed in hematopoietic cells as well as epithelia. The function of hematopoietic E-cadherin is, however, mostly elusive. In this study, we explored the validity of mouse models to functionally investigate the role of hematopoietic E-cadherin in human hematopoiesis. We generated a hematopoietic-specific E-cadherin knockout mouse model. In mice, hematopoietic E-cadherin is predominantly expressed within the basophil lineage, the expression of which is dispensable for the generation of basophils. However, neither E-cadherin mRNA nor protein were detected in human basophils. In contrast, human hematopoietic E-cadherin marks the erythroid lineage. E-cadherin expression in hematopoiesis thereby revealed striking evolutionary differences between the basophil and erythroid cell lineage in humans and mice. This is remarkable as E-cadherin expression in epithelia is highly conserved among vertebrates including humans and mice. Our study therefore revealed that the mouse does not represent a suitable model to study the function of E-cadherin in human hematopoiesis and an alternative means to study the role of E-cadherin in human erythropoiesis needs to be developed. |
| first_indexed | 2024-03-09T18:27:38Z |
| format | Article |
| id | doaj.art-a914931423c94e2dab2c0c3a66e2890f |
| institution | Directory Open Access Journal |
| issn | 2218-273X |
| language | English |
| last_indexed | 2024-03-09T18:27:38Z |
| publishDate | 2022-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Biomolecules |
| spelling | doaj.art-a914931423c94e2dab2c0c3a66e2890f2023-11-24T07:48:06ZengMDPI AGBiomolecules2218-273X2022-11-011211170610.3390/biom12111706E-Cadherin Expression Distinguishes Mouse from Human Hematopoiesis in the Basophil and Erythroid LineagesRosa A. Krimpenfort0Felix M. Behr1Marja Nieuwland2Iris de Rink3Ron Kerkhoven4Marieke von Lindern5Micha Nethe6Sanquin Research, Landsteiner Laboratory, Academic Medical Centre, Department of Hematopoiesis, University of Amsterdam, 1066 CX Amsterdam, The NetherlandsSanquin Research, Landsteiner Laboratory, Academic Medical Centre, Department of Hematopoiesis, University of Amsterdam, 1066 CX Amsterdam, The NetherlandsGenomics Core Facility, Netherlands Cancer Institute, 1006 BE Amsterdam, The NetherlandsGenomics Core Facility, Netherlands Cancer Institute, 1006 BE Amsterdam, The NetherlandsGenomics Core Facility, Netherlands Cancer Institute, 1006 BE Amsterdam, The NetherlandsSanquin Research, Landsteiner Laboratory, Academic Medical Centre, Department of Hematopoiesis, University of Amsterdam, 1066 CX Amsterdam, The NetherlandsSanquin Research, Landsteiner Laboratory, Academic Medical Centre, Department of Hematopoiesis, University of Amsterdam, 1066 CX Amsterdam, The NetherlandsE-cadherin is a key regulator of epithelial cell–cell adhesion, the loss of which accelerates tumor growth and invasion. E-cadherin is also expressed in hematopoietic cells as well as epithelia. The function of hematopoietic E-cadherin is, however, mostly elusive. In this study, we explored the validity of mouse models to functionally investigate the role of hematopoietic E-cadherin in human hematopoiesis. We generated a hematopoietic-specific E-cadherin knockout mouse model. In mice, hematopoietic E-cadherin is predominantly expressed within the basophil lineage, the expression of which is dispensable for the generation of basophils. However, neither E-cadherin mRNA nor protein were detected in human basophils. In contrast, human hematopoietic E-cadherin marks the erythroid lineage. E-cadherin expression in hematopoiesis thereby revealed striking evolutionary differences between the basophil and erythroid cell lineage in humans and mice. This is remarkable as E-cadherin expression in epithelia is highly conserved among vertebrates including humans and mice. Our study therefore revealed that the mouse does not represent a suitable model to study the function of E-cadherin in human hematopoiesis and an alternative means to study the role of E-cadherin in human erythropoiesis needs to be developed.https://www.mdpi.com/2218-273X/12/11/1706E-cadherinerythropoiesishematopoiesisbasophilerythroblast |
| spellingShingle | Rosa A. Krimpenfort Felix M. Behr Marja Nieuwland Iris de Rink Ron Kerkhoven Marieke von Lindern Micha Nethe E-Cadherin Expression Distinguishes Mouse from Human Hematopoiesis in the Basophil and Erythroid Lineages Biomolecules E-cadherin erythropoiesis hematopoiesis basophil erythroblast |
| title | E-Cadherin Expression Distinguishes Mouse from Human Hematopoiesis in the Basophil and Erythroid Lineages |
| title_full | E-Cadherin Expression Distinguishes Mouse from Human Hematopoiesis in the Basophil and Erythroid Lineages |
| title_fullStr | E-Cadherin Expression Distinguishes Mouse from Human Hematopoiesis in the Basophil and Erythroid Lineages |
| title_full_unstemmed | E-Cadherin Expression Distinguishes Mouse from Human Hematopoiesis in the Basophil and Erythroid Lineages |
| title_short | E-Cadherin Expression Distinguishes Mouse from Human Hematopoiesis in the Basophil and Erythroid Lineages |
| title_sort | e cadherin expression distinguishes mouse from human hematopoiesis in the basophil and erythroid lineages |
| topic | E-cadherin erythropoiesis hematopoiesis basophil erythroblast |
| url | https://www.mdpi.com/2218-273X/12/11/1706 |
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