Unintended Changes in Genetically Modified Rice Expressing the Lysine-Rich Fusion Protein Gene Revealed by a Proteomics Approach
Development of new technologies for evaluating genetically modified (GM) crops has revealed that there are unintended insertions and expression changes in GM crops. Profiling techniques are non-targeted approaches and are capable of detecting more unintended changes in GM crops. Here, we report the...
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| Format: | Article |
| Language: | English |
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Elsevier
2013-11-01
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| Series: | Journal of Integrative Agriculture |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2095311913605390 |
| _version_ | 1818642532291051520 |
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| author | Xiang-xiang ZHAO Tang TANG Fu-xia LIU Chang-li LU Xiao-lan HU Li-lian JI Qiao-quan LIU |
| author_facet | Xiang-xiang ZHAO Tang TANG Fu-xia LIU Chang-li LU Xiao-lan HU Li-lian JI Qiao-quan LIU |
| author_sort | Xiang-xiang ZHAO |
| collection | DOAJ |
| description | Development of new technologies for evaluating genetically modified (GM) crops has revealed that there are unintended insertions and expression changes in GM crops. Profiling techniques are non-targeted approaches and are capable of detecting more unintended changes in GM crops. Here, we report the application of a comparative proteomic approach to investigate the protein profile differences between a GM rice line, which has a lysine-rich protein gene, and its non-transgenic parental line. Proteome analysis by two-dimensional gel electrophoresis (2-DE) and mass spectrum analysis of the seeds identified 22 differentially expressed protein spots. Apart from a number of glutelins that were detected as targeted proteins in the GM line, the majority of the other changed proteins were involved in carbohydrate metabolism, protein synthesis and stress responses. These results indicated that the altered proteins were not associated with plant allergens or toxicity. |
| first_indexed | 2024-12-16T23:44:33Z |
| format | Article |
| id | doaj.art-a916853ff8e849fcb881117a16005182 |
| institution | Directory Open Access Journal |
| issn | 2095-3119 |
| language | English |
| last_indexed | 2024-12-16T23:44:33Z |
| publishDate | 2013-11-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Integrative Agriculture |
| spelling | doaj.art-a916853ff8e849fcb881117a160051822022-12-21T22:11:33ZengElsevierJournal of Integrative Agriculture2095-31192013-11-01121120132021Unintended Changes in Genetically Modified Rice Expressing the Lysine-Rich Fusion Protein Gene Revealed by a Proteomics ApproachXiang-xiang ZHAO0Tang TANG1Fu-xia LIU2Chang-li LU3Xiao-lan HU4Li-lian JI5Qiao-quan LIU6Jiangsu Key Laboratory for Eco-Agricultural Biotechnology Around Hongze Lake, Huaiyin Normal University, Huaian 223300, P.R. ChinaJiangsu Key Laboratory for Eco-Agricultural Biotechnology Around Hongze Lake, Huaiyin Normal University, Huaian 223300, P.R. ChinaJiangsu Key Laboratory for Eco-Agricultural Biotechnology Around Hongze Lake, Huaiyin Normal University, Huaian 223300, P.R. ChinaJiangsu Key Laboratory for Eco-Agricultural Biotechnology Around Hongze Lake, Huaiyin Normal University, Huaian 223300, P.R. ChinaJiangsu Key Laboratory for Eco-Agricultural Biotechnology Around Hongze Lake, Huaiyin Normal University, Huaian 223300, P.R. ChinaJiangsu Key Laboratory for Eco-Agricultural Biotechnology Around Hongze Lake, Huaiyin Normal University, Huaian 223300, P.R. China; Correspondence JI Li-lian, Tel/Fax: +86-517-83525885Key Laboratories of Crop Genetics and Physiology of the Jiangsu Province and Plant Functional Genomics, Ministry of Education/Yangzhou University, Yangzhou 225009, P.R. China; LIU Qiao-quan, Tel/Fax: +86-514-87996648Development of new technologies for evaluating genetically modified (GM) crops has revealed that there are unintended insertions and expression changes in GM crops. Profiling techniques are non-targeted approaches and are capable of detecting more unintended changes in GM crops. Here, we report the application of a comparative proteomic approach to investigate the protein profile differences between a GM rice line, which has a lysine-rich protein gene, and its non-transgenic parental line. Proteome analysis by two-dimensional gel electrophoresis (2-DE) and mass spectrum analysis of the seeds identified 22 differentially expressed protein spots. Apart from a number of glutelins that were detected as targeted proteins in the GM line, the majority of the other changed proteins were involved in carbohydrate metabolism, protein synthesis and stress responses. These results indicated that the altered proteins were not associated with plant allergens or toxicity.http://www.sciencedirect.com/science/article/pii/S2095311913605390transgenic riceproteomeprofiling techniquesunintended changestwo-dimensional gel electrophoresis |
| spellingShingle | Xiang-xiang ZHAO Tang TANG Fu-xia LIU Chang-li LU Xiao-lan HU Li-lian JI Qiao-quan LIU Unintended Changes in Genetically Modified Rice Expressing the Lysine-Rich Fusion Protein Gene Revealed by a Proteomics Approach Journal of Integrative Agriculture transgenic rice proteome profiling techniques unintended changes two-dimensional gel electrophoresis |
| title | Unintended Changes in Genetically Modified Rice Expressing the Lysine-Rich Fusion Protein Gene Revealed by a Proteomics Approach |
| title_full | Unintended Changes in Genetically Modified Rice Expressing the Lysine-Rich Fusion Protein Gene Revealed by a Proteomics Approach |
| title_fullStr | Unintended Changes in Genetically Modified Rice Expressing the Lysine-Rich Fusion Protein Gene Revealed by a Proteomics Approach |
| title_full_unstemmed | Unintended Changes in Genetically Modified Rice Expressing the Lysine-Rich Fusion Protein Gene Revealed by a Proteomics Approach |
| title_short | Unintended Changes in Genetically Modified Rice Expressing the Lysine-Rich Fusion Protein Gene Revealed by a Proteomics Approach |
| title_sort | unintended changes in genetically modified rice expressing the lysine rich fusion protein gene revealed by a proteomics approach |
| topic | transgenic rice proteome profiling techniques unintended changes two-dimensional gel electrophoresis |
| url | http://www.sciencedirect.com/science/article/pii/S2095311913605390 |
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