Optimization of Anthocyanin Production in Tobacco Cells
Plant cell cultures have emerged as a promising tool for producing active molecules due to their numerous advantages over traditional agricultural methods. Flavonols, and anthocyanin pigments in particular, together with other phenolic compounds such as chlorogenic acid, are known for their benefici...
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-09-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/24/18/13711 |
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| author | Andrea Carpi Md Abdur Rahim Angela Marin Marco Armellin Paola Brun Giovanni Miotto Renzo Dal Monte Livio Trainotti |
| author_facet | Andrea Carpi Md Abdur Rahim Angela Marin Marco Armellin Paola Brun Giovanni Miotto Renzo Dal Monte Livio Trainotti |
| author_sort | Andrea Carpi |
| collection | DOAJ |
| description | Plant cell cultures have emerged as a promising tool for producing active molecules due to their numerous advantages over traditional agricultural methods. Flavonols, and anthocyanin pigments in particular, together with other phenolic compounds such as chlorogenic acid, are known for their beneficial health properties, mainly due to their antioxidant, antimicrobial, and anti-inflammatory activities. The synthesis of these molecules is finely regulated in plant cells and controlled at the transcriptional level by specific MYB and bHLH transcription factors that coordinate the transcription of structural biosynthetic genes. The co-expression of peach <i>PpMYB10.1</i> and <i>PpbHLH3</i> in tobacco was used to develop tobacco cell lines showing high expression of both the peach transgenes and the native flavonol structural genes. These cell lines were further selected for fast growth. High production levels of chlorogenic acid, anthocyanins (mainly cyanidin 3-rutinoside), and other phenolics were also achieved in pre-industrial scale-up trials. A single-column-based purification protocol was developed to produce a lyophile called ANT-CA, which was stable over time, showed beneficial effects on cell viability, and had antioxidant, anti-inflammatory, antibacterial, and wound-healing activities. This lyophile could be a valuable ingredient for food or cosmetic applications. |
| first_indexed | 2024-03-10T22:41:27Z |
| format | Article |
| id | doaj.art-a9179d7edae045adbe7d562f987167af |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-10T22:41:27Z |
| publishDate | 2023-09-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-a9179d7edae045adbe7d562f987167af2023-11-19T11:01:59ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672023-09-0124181371110.3390/ijms241813711Optimization of Anthocyanin Production in Tobacco CellsAndrea Carpi0Md Abdur Rahim1Angela Marin2Marco Armellin3Paola Brun4Giovanni Miotto5Renzo Dal Monte6Livio Trainotti7Active Botanicals Research (ABR), 36040 Brendola, ItalyDepartment of Biology, University of Padua, 35131 Padua, ItalyActive Botanicals Research (ABR), 36040 Brendola, ItalyDepartment of Biology, University of Padua, 35131 Padua, ItalyDepartment of Molecular Medicine, University of Padua, 35131 Padua, ItalyDepartment of Molecular Medicine, University of Padua, 35131 Padua, ItalyActive Botanicals Research (ABR), 36040 Brendola, ItalyDepartment of Biology, University of Padua, 35131 Padua, ItalyPlant cell cultures have emerged as a promising tool for producing active molecules due to their numerous advantages over traditional agricultural methods. Flavonols, and anthocyanin pigments in particular, together with other phenolic compounds such as chlorogenic acid, are known for their beneficial health properties, mainly due to their antioxidant, antimicrobial, and anti-inflammatory activities. The synthesis of these molecules is finely regulated in plant cells and controlled at the transcriptional level by specific MYB and bHLH transcription factors that coordinate the transcription of structural biosynthetic genes. The co-expression of peach <i>PpMYB10.1</i> and <i>PpbHLH3</i> in tobacco was used to develop tobacco cell lines showing high expression of both the peach transgenes and the native flavonol structural genes. These cell lines were further selected for fast growth. High production levels of chlorogenic acid, anthocyanins (mainly cyanidin 3-rutinoside), and other phenolics were also achieved in pre-industrial scale-up trials. A single-column-based purification protocol was developed to produce a lyophile called ANT-CA, which was stable over time, showed beneficial effects on cell viability, and had antioxidant, anti-inflammatory, antibacterial, and wound-healing activities. This lyophile could be a valuable ingredient for food or cosmetic applications.https://www.mdpi.com/1422-0067/24/18/13711<i>Nicotiana tabacum</i><i>Prunus persica</i>synthetic biology |
| spellingShingle | Andrea Carpi Md Abdur Rahim Angela Marin Marco Armellin Paola Brun Giovanni Miotto Renzo Dal Monte Livio Trainotti Optimization of Anthocyanin Production in Tobacco Cells International Journal of Molecular Sciences <i>Nicotiana tabacum</i> <i>Prunus persica</i> synthetic biology |
| title | Optimization of Anthocyanin Production in Tobacco Cells |
| title_full | Optimization of Anthocyanin Production in Tobacco Cells |
| title_fullStr | Optimization of Anthocyanin Production in Tobacco Cells |
| title_full_unstemmed | Optimization of Anthocyanin Production in Tobacco Cells |
| title_short | Optimization of Anthocyanin Production in Tobacco Cells |
| title_sort | optimization of anthocyanin production in tobacco cells |
| topic | <i>Nicotiana tabacum</i> <i>Prunus persica</i> synthetic biology |
| url | https://www.mdpi.com/1422-0067/24/18/13711 |
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