| Summary: | Reactive oxygen species (ROS) produced during stress responses are implicated
in a number of cellular responses including morphogenesis. The present study
was undertaken to study the changes in antioxidative enzymes during in vitro
morphogenesis of groundnut from de-embryonated cotyledon explants cultured on
Murashige and Skoog’s medium supplemented with 5.0 mg l-1 benzyl-adenine and
2.0 mg l-1 2,4-dichlorophenoxyacetic acid. During the early in vitro
ontogenic stages of groundnut, the activity of peroxidase (POD) and
polyphenol oxidase (PPO) increased from stage 0 (0 day) to stage II (14 days)
and decreased during stage III (25 days) and stage IV (45 days). The activity
of superoxide dismutase (SOD) showed an inverse trend. The results could be
correlated with the acquisition of competence, de-differentiation, division
and induction which occurred during shoot organogenesis. Histological studies
also showed that the mode of in vitro morphogenesis from the groundnut
explants was via shoot organogenesis. In light of the above study, it could
be concluded that the change in activity of the antioxidative enzymes studied
could be used as a marker to characterize the mode of plant regeneration.
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