Re-Emergence of Salmonellosis in Hog Farms: Outbreak and Bacteriological Characterization
Clinical salmonellosis has been increasing significantly in Brazil in recent years. A total of 130 outbreaks distributed among 10 swine-producing states were investigated. One representative <i>Salmonella</i> isolate from each outbreak was characterized through serotyping, antimicrobial...
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MDPI AG
2021-04-01
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| Series: | Microorganisms |
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| Online Access: | https://www.mdpi.com/2076-2607/9/5/947 |
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| author | Mariana Meneguzzi Caroline Pissetti Raquel Rebelatto Julian Trachsel Suzana Satomi Kuchiishi Adrienny Trindade Reis Roberto Maurício Carvalho Guedes Joice Aparecida Leão Caroline Reichen Jalusa Deon Kich |
| author_facet | Mariana Meneguzzi Caroline Pissetti Raquel Rebelatto Julian Trachsel Suzana Satomi Kuchiishi Adrienny Trindade Reis Roberto Maurício Carvalho Guedes Joice Aparecida Leão Caroline Reichen Jalusa Deon Kich |
| author_sort | Mariana Meneguzzi |
| collection | DOAJ |
| description | Clinical salmonellosis has been increasing significantly in Brazil in recent years. A total of 130 outbreaks distributed among 10 swine-producing states were investigated. One representative <i>Salmonella</i> isolate from each outbreak was characterized through serotyping, antimicrobial resistance profiles, PFGE, and WGS. From 130 outbreaks: 50 were enteric, 48 were septicemic, 17 cases were characterized as hepato-biliary invasive, 13 as nodal and two were not classified. The most prevalent serovars were a monophasic variant of <i>S. typhimurium</i> (55/130), Choleraesuis (46/130), and Typhimurium (14/130). Most of the strains (86.92%) demonstrated a high rate of multi-drug resistance. The identification of a major Choleraesuis clonal group in several Brazilian states sharing the same resistance genes suggested that these strains were closely related. Six strains from this clonal group were sequenced, revealing the same ST-145 and 11 to 47 different SNPs. The detected plasmid type showed multiple marker genes as RepA_1_pKPC-CAV1321, the first to be reported in <i>Salmonella</i>. All AMR genes detected in the genomes were likely present on plasmids, and their phenotype was related to genotypic resistance genes. These findings reveal that salmonellosis is endemic in the most important pig-producing states in Brazil, emphasizing the need to make data available to aid in reducing its occurrence. |
| first_indexed | 2024-03-10T11:54:22Z |
| format | Article |
| id | doaj.art-a91944b25190472d999634a703e35568 |
| institution | Directory Open Access Journal |
| issn | 2076-2607 |
| language | English |
| last_indexed | 2024-03-10T11:54:22Z |
| publishDate | 2021-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Microorganisms |
| spelling | doaj.art-a91944b25190472d999634a703e355682023-11-21T17:27:58ZengMDPI AGMicroorganisms2076-26072021-04-019594710.3390/microorganisms9050947Re-Emergence of Salmonellosis in Hog Farms: Outbreak and Bacteriological CharacterizationMariana Meneguzzi0Caroline Pissetti1Raquel Rebelatto2Julian Trachsel3Suzana Satomi Kuchiishi4Adrienny Trindade Reis5Roberto Maurício Carvalho Guedes6Joice Aparecida Leão7Caroline Reichen8Jalusa Deon Kich9Curso de Medicina Veterinária, Instituto Federal Catarinense, Concórdia 89703-720, SC, BrazilDepartamento de Medicina Veterinária Preventiva, Faculdade de Veterinária, Universidade Federal do Rio Grande do Sul, Porto Alegre 91540-000, RS, BrazilEmbrapa Suínos e Aves, Empresa Brasileira de Pesquisa Agropecuária, Concórdia 89715-899, SC, BrazilNational Animal Disease Center, Food Safety and Enteric Pathogens, Ames, IA 50010, USACentro de Diagnóstico de Sanidade Animal, CEDISA, Concórdia 89715-899, SC, BrazilCentro de Diagnóstico e Monitoramento Animal, CDMA, Belo Horizonte 30411-191, MG, BrazilDepartamento de Clínica e Cirurgia Veterinária, Escola de Veterinária, Universidade Federal de Minas Gerais, Belo Horizonte 31270-901, MG, BrazilLaboratório Integralab, Cascavel 85816-430, PR, BrazilCurso de Medicina Veterinária, Instituto Federal Catarinense, Concórdia 89703-720, SC, BrazilEmbrapa Suínos e Aves, Empresa Brasileira de Pesquisa Agropecuária, Concórdia 89715-899, SC, BrazilClinical salmonellosis has been increasing significantly in Brazil in recent years. A total of 130 outbreaks distributed among 10 swine-producing states were investigated. One representative <i>Salmonella</i> isolate from each outbreak was characterized through serotyping, antimicrobial resistance profiles, PFGE, and WGS. From 130 outbreaks: 50 were enteric, 48 were septicemic, 17 cases were characterized as hepato-biliary invasive, 13 as nodal and two were not classified. The most prevalent serovars were a monophasic variant of <i>S. typhimurium</i> (55/130), Choleraesuis (46/130), and Typhimurium (14/130). Most of the strains (86.92%) demonstrated a high rate of multi-drug resistance. The identification of a major Choleraesuis clonal group in several Brazilian states sharing the same resistance genes suggested that these strains were closely related. Six strains from this clonal group were sequenced, revealing the same ST-145 and 11 to 47 different SNPs. The detected plasmid type showed multiple marker genes as RepA_1_pKPC-CAV1321, the first to be reported in <i>Salmonella</i>. All AMR genes detected in the genomes were likely present on plasmids, and their phenotype was related to genotypic resistance genes. These findings reveal that salmonellosis is endemic in the most important pig-producing states in Brazil, emphasizing the need to make data available to aid in reducing its occurrence.https://www.mdpi.com/2076-2607/9/5/947<i>Salmonella</i>swinePFGEMLSTWGS |
| spellingShingle | Mariana Meneguzzi Caroline Pissetti Raquel Rebelatto Julian Trachsel Suzana Satomi Kuchiishi Adrienny Trindade Reis Roberto Maurício Carvalho Guedes Joice Aparecida Leão Caroline Reichen Jalusa Deon Kich Re-Emergence of Salmonellosis in Hog Farms: Outbreak and Bacteriological Characterization Microorganisms <i>Salmonella</i> swine PFGE MLST WGS |
| title | Re-Emergence of Salmonellosis in Hog Farms: Outbreak and Bacteriological Characterization |
| title_full | Re-Emergence of Salmonellosis in Hog Farms: Outbreak and Bacteriological Characterization |
| title_fullStr | Re-Emergence of Salmonellosis in Hog Farms: Outbreak and Bacteriological Characterization |
| title_full_unstemmed | Re-Emergence of Salmonellosis in Hog Farms: Outbreak and Bacteriological Characterization |
| title_short | Re-Emergence of Salmonellosis in Hog Farms: Outbreak and Bacteriological Characterization |
| title_sort | re emergence of salmonellosis in hog farms outbreak and bacteriological characterization |
| topic | <i>Salmonella</i> swine PFGE MLST WGS |
| url | https://www.mdpi.com/2076-2607/9/5/947 |
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