Monitoring persistence of the entomopathogenic fungus Metarhizium anisopliae under simulated field conditions with the aim of controlling adult Aedes aegypti (Diptera: Culicidae)
Abstract Background Entomopathogenic fungi are potential candidates for use in integrated vector management, with recent emphasis aimed at developing adult mosquito control methods. Here we investigated the persistence of the fungus Metarhizium anisopliae when tested against female A. aegypti under...
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
BMC
2014-04-01
|
| Series: | Parasites & Vectors |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/1756-3305-7-198 |
| _version_ | 1797811702748676096 |
|---|---|
| author | Aline T Carolino Adriano R Paula Carlos P Silva Tariq M Butt Richard I Samuels |
| author_facet | Aline T Carolino Adriano R Paula Carlos P Silva Tariq M Butt Richard I Samuels |
| author_sort | Aline T Carolino |
| collection | DOAJ |
| description | Abstract Background Entomopathogenic fungi are potential candidates for use in integrated vector management, with recent emphasis aimed at developing adult mosquito control methods. Here we investigated the persistence of the fungus Metarhizium anisopliae when tested against female A. aegypti under field conditions. Methods Black cotton cloths impregnated with M. anisopliae conidia, formulated in vegetable oil + isoparaffin, were maintained on a covered veranda for up to 30 days. At specific times, pieces of the cloths were removed, placed in Tween 80 and the resuspended conidia were sprayed directly onto mosquitoes. The persistence of conidia impregnated on black cloths using three different carriers was evaluated in test rooms. Fifty mosquitoes were released into each room and after a 5 day period, the surviving insects were captured. Another 50 insects were then released into each room. The capacity of the fungus at reducing mosquito survival was evaluated over a total of 35 days. Results Conidia extracted from cloths maintained on the veranda for 2 to 18 days remained virulent, with 28 to 60% mosquito survival observed. Mosquito survival following exposure to fungus impregnated cloths showed that fungus + Tween caused similar reductions to that of fungus + vegetable oil. Mosquitoes exposed to the formulation fungus + vegetable oil had survival rates of 36% over the first 5 days of the experiment. Following the release of the second cohort of mosquitoes (6-11days), survival increased to 50%. The survival of the 12–17 day cohort (78%) was statistically equal to that of the controls (84%). Formulation of the fungus in vegetable oil + isoparaffin increased the persistence of the fungus, with the 18–23 day cohort (64% survival) still showing statistical differences to that of the controls (87% survival). Conclusions The potential of entomopathogenic fungi for the control of adult A. aegypti was confirmed under field conditions. Vegetable oil + isoparaffin formulations of M. anisopliae significantly increased the effectiveness of the fungus, thus reducing the need for frequent changes of black cloths in residences. Our future aim is to obtain effective control of mosquito populations, with cloths only needing to being replaced once a month. |
| first_indexed | 2024-03-13T07:27:39Z |
| format | Article |
| id | doaj.art-a91c6007745d4afb8b7b6fbb6d246964 |
| institution | Directory Open Access Journal |
| issn | 1756-3305 |
| language | English |
| last_indexed | 2024-03-13T07:27:39Z |
| publishDate | 2014-04-01 |
| publisher | BMC |
| record_format | Article |
| series | Parasites & Vectors |
| spelling | doaj.art-a91c6007745d4afb8b7b6fbb6d2469642023-06-04T11:17:56ZengBMCParasites & Vectors1756-33052014-04-01711710.1186/1756-3305-7-198Monitoring persistence of the entomopathogenic fungus Metarhizium anisopliae under simulated field conditions with the aim of controlling adult Aedes aegypti (Diptera: Culicidae)Aline T Carolino0Adriano R Paula1Carlos P Silva2Tariq M Butt3Richard I Samuels4Department of Entomology and Plant Pathology, Universidade Estadual do Norte Fluminense Darcy RibeiroDepartment of Entomology and Plant Pathology, Universidade Estadual do Norte Fluminense Darcy RibeiroDepartamento de Bioquímica, Universidade Federal de Santa CatarinaDepartment of Biosciences, Swansea UniversityDepartment of Entomology and Plant Pathology, Universidade Estadual do Norte Fluminense Darcy RibeiroAbstract Background Entomopathogenic fungi are potential candidates for use in integrated vector management, with recent emphasis aimed at developing adult mosquito control methods. Here we investigated the persistence of the fungus Metarhizium anisopliae when tested against female A. aegypti under field conditions. Methods Black cotton cloths impregnated with M. anisopliae conidia, formulated in vegetable oil + isoparaffin, were maintained on a covered veranda for up to 30 days. At specific times, pieces of the cloths were removed, placed in Tween 80 and the resuspended conidia were sprayed directly onto mosquitoes. The persistence of conidia impregnated on black cloths using three different carriers was evaluated in test rooms. Fifty mosquitoes were released into each room and after a 5 day period, the surviving insects were captured. Another 50 insects were then released into each room. The capacity of the fungus at reducing mosquito survival was evaluated over a total of 35 days. Results Conidia extracted from cloths maintained on the veranda for 2 to 18 days remained virulent, with 28 to 60% mosquito survival observed. Mosquito survival following exposure to fungus impregnated cloths showed that fungus + Tween caused similar reductions to that of fungus + vegetable oil. Mosquitoes exposed to the formulation fungus + vegetable oil had survival rates of 36% over the first 5 days of the experiment. Following the release of the second cohort of mosquitoes (6-11days), survival increased to 50%. The survival of the 12–17 day cohort (78%) was statistically equal to that of the controls (84%). Formulation of the fungus in vegetable oil + isoparaffin increased the persistence of the fungus, with the 18–23 day cohort (64% survival) still showing statistical differences to that of the controls (87% survival). Conclusions The potential of entomopathogenic fungi for the control of adult A. aegypti was confirmed under field conditions. Vegetable oil + isoparaffin formulations of M. anisopliae significantly increased the effectiveness of the fungus, thus reducing the need for frequent changes of black cloths in residences. Our future aim is to obtain effective control of mosquito populations, with cloths only needing to being replaced once a month.https://doi.org/10.1186/1756-3305-7-198PersistenceViabilityVectorDengueOil formulation |
| spellingShingle | Aline T Carolino Adriano R Paula Carlos P Silva Tariq M Butt Richard I Samuels Monitoring persistence of the entomopathogenic fungus Metarhizium anisopliae under simulated field conditions with the aim of controlling adult Aedes aegypti (Diptera: Culicidae) Parasites & Vectors Persistence Viability Vector Dengue Oil formulation |
| title | Monitoring persistence of the entomopathogenic fungus Metarhizium anisopliae under simulated field conditions with the aim of controlling adult Aedes aegypti (Diptera: Culicidae) |
| title_full | Monitoring persistence of the entomopathogenic fungus Metarhizium anisopliae under simulated field conditions with the aim of controlling adult Aedes aegypti (Diptera: Culicidae) |
| title_fullStr | Monitoring persistence of the entomopathogenic fungus Metarhizium anisopliae under simulated field conditions with the aim of controlling adult Aedes aegypti (Diptera: Culicidae) |
| title_full_unstemmed | Monitoring persistence of the entomopathogenic fungus Metarhizium anisopliae under simulated field conditions with the aim of controlling adult Aedes aegypti (Diptera: Culicidae) |
| title_short | Monitoring persistence of the entomopathogenic fungus Metarhizium anisopliae under simulated field conditions with the aim of controlling adult Aedes aegypti (Diptera: Culicidae) |
| title_sort | monitoring persistence of the entomopathogenic fungus metarhizium anisopliae under simulated field conditions with the aim of controlling adult aedes aegypti diptera culicidae |
| topic | Persistence Viability Vector Dengue Oil formulation |
| url | https://doi.org/10.1186/1756-3305-7-198 |
| work_keys_str_mv | AT alinetcarolino monitoringpersistenceoftheentomopathogenicfungusmetarhiziumanisopliaeundersimulatedfieldconditionswiththeaimofcontrollingadultaedesaegyptidipteraculicidae AT adrianorpaula monitoringpersistenceoftheentomopathogenicfungusmetarhiziumanisopliaeundersimulatedfieldconditionswiththeaimofcontrollingadultaedesaegyptidipteraculicidae AT carlospsilva monitoringpersistenceoftheentomopathogenicfungusmetarhiziumanisopliaeundersimulatedfieldconditionswiththeaimofcontrollingadultaedesaegyptidipteraculicidae AT tariqmbutt monitoringpersistenceoftheentomopathogenicfungusmetarhiziumanisopliaeundersimulatedfieldconditionswiththeaimofcontrollingadultaedesaegyptidipteraculicidae AT richardisamuels monitoringpersistenceoftheentomopathogenicfungusmetarhiziumanisopliaeundersimulatedfieldconditionswiththeaimofcontrollingadultaedesaegyptidipteraculicidae |