The diagnostic combination of serum circulating miR-488 and lncRNA AC018761 as biomarkers for hypopharyngeal squamous cell carcinoma (HPSCC)
Objective: The search for rapid and effective early diagnostic markers of hypotharyngeal carcinoma becomes particularly critical and important. Therefore, we explored the value of circulating expression of non-coding RNAs for the diagnosis of early hypotharyngeal cancer. Methods: After screening HPS...
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| Format: | Article |
| Language: | English |
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Elsevier
2023-08-01
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| Series: | Arabian Journal of Chemistry |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S1878535223003714 |
| _version_ | 1797804011675451392 |
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| author | Jiahong Pei Jinqian Zhang Tianshu Li Cun Feng Yanfei Guan Shunmin Gong Xianbao Cao |
| author_facet | Jiahong Pei Jinqian Zhang Tianshu Li Cun Feng Yanfei Guan Shunmin Gong Xianbao Cao |
| author_sort | Jiahong Pei |
| collection | DOAJ |
| description | Objective: The search for rapid and effective early diagnostic markers of hypotharyngeal carcinoma becomes particularly critical and important. Therefore, we explored the value of circulating expression of non-coding RNAs for the diagnosis of early hypotharyngeal cancer. Methods: After screening HPSCC-related research data in the NCBI-GEO database, the practical report extraction language (Perl) was used to give genes different biotype attributes. The R language limma package was utilized to analyze the differential expression of lncRNAs and mRNAs, respectively.The quantitative RT-PCR (Reverse transcription-polymerase chain reaction) analysis were performed to determine the plasma expression levels of non-coding RNAs Results: Compared with the control groups, the differential expression of 137 lncRNAs and 297 mRNAs in the plasma of HPSCC patients was statistically significant. There were 32 differentially expressed lncRNAs, 19 differentially expressed mRNAs. The score of LncRNA AC018761 + miR-488 was AUC = 0.846, the best cut-off value was ≥ 40 points, the sensitivity was 74.58%, and the specificity was 79.88%. Conclusion: Our results indicated that the over-expression of lncRNA-AC018761 and miR-488 were significantly up-regulated in serum of patients with HPSCC. Therefore, lncRNA-AC018761 and miR-488 maybe the non-invasive independent predictor for early HPSCC with high sensitivity and specificity. |
| first_indexed | 2024-03-13T05:29:41Z |
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| id | doaj.art-a91d96d4cdd14dce91018206cd395201 |
| institution | Directory Open Access Journal |
| issn | 1878-5352 |
| language | English |
| last_indexed | 2024-03-13T05:29:41Z |
| publishDate | 2023-08-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Arabian Journal of Chemistry |
| spelling | doaj.art-a91d96d4cdd14dce91018206cd3952012023-06-15T04:54:53ZengElsevierArabian Journal of Chemistry1878-53522023-08-01168104909The diagnostic combination of serum circulating miR-488 and lncRNA AC018761 as biomarkers for hypopharyngeal squamous cell carcinoma (HPSCC)Jiahong Pei0Jinqian Zhang1Tianshu Li2Cun Feng3Yanfei Guan4Shunmin Gong5Xianbao Cao6Department of Otolaryngology Head and Neck Surgery, The First People’s Hospital of Yunnan Province, The Affiliated Hospital of Kunming University of Science and Technology, Kunming 650032, Yunnan Province, ChinaCorresponding authors.; Department of Otolaryngology Head and Neck Surgery, The First People’s Hospital of Yunnan Province, The Affiliated Hospital of Kunming University of Science and Technology, Kunming 650032, Yunnan Province, ChinaDepartment of Otolaryngology Head and Neck Surgery, The First People’s Hospital of Yunnan Province, The Affiliated Hospital of Kunming University of Science and Technology, Kunming 650032, Yunnan Province, ChinaDepartment of Otolaryngology Head and Neck Surgery, The First People’s Hospital of Yunnan Province, The Affiliated Hospital of Kunming University of Science and Technology, Kunming 650032, Yunnan Province, ChinaDepartment of Otolaryngology Head and Neck Surgery, The First People’s Hospital of Yunnan Province, The Affiliated Hospital of Kunming University of Science and Technology, Kunming 650032, Yunnan Province, ChinaDepartment of Otolaryngology Head and Neck Surgery, The First People’s Hospital of Yunnan Province, The Affiliated Hospital of Kunming University of Science and Technology, Kunming 650032, Yunnan Province, ChinaCorresponding authors.; Department of Otolaryngology Head and Neck Surgery, The First People’s Hospital of Yunnan Province, The Affiliated Hospital of Kunming University of Science and Technology, Kunming 650032, Yunnan Province, ChinaObjective: The search for rapid and effective early diagnostic markers of hypotharyngeal carcinoma becomes particularly critical and important. Therefore, we explored the value of circulating expression of non-coding RNAs for the diagnosis of early hypotharyngeal cancer. Methods: After screening HPSCC-related research data in the NCBI-GEO database, the practical report extraction language (Perl) was used to give genes different biotype attributes. The R language limma package was utilized to analyze the differential expression of lncRNAs and mRNAs, respectively.The quantitative RT-PCR (Reverse transcription-polymerase chain reaction) analysis were performed to determine the plasma expression levels of non-coding RNAs Results: Compared with the control groups, the differential expression of 137 lncRNAs and 297 mRNAs in the plasma of HPSCC patients was statistically significant. There were 32 differentially expressed lncRNAs, 19 differentially expressed mRNAs. The score of LncRNA AC018761 + miR-488 was AUC = 0.846, the best cut-off value was ≥ 40 points, the sensitivity was 74.58%, and the specificity was 79.88%. Conclusion: Our results indicated that the over-expression of lncRNA-AC018761 and miR-488 were significantly up-regulated in serum of patients with HPSCC. Therefore, lncRNA-AC018761 and miR-488 maybe the non-invasive independent predictor for early HPSCC with high sensitivity and specificity.http://www.sciencedirect.com/science/article/pii/S1878535223003714Circulating non-coding RNAshypopharyngeal squamous cell carcinoma (HPSCC)BiomarkerNon-invasive diagnosis (NID) |
| spellingShingle | Jiahong Pei Jinqian Zhang Tianshu Li Cun Feng Yanfei Guan Shunmin Gong Xianbao Cao The diagnostic combination of serum circulating miR-488 and lncRNA AC018761 as biomarkers for hypopharyngeal squamous cell carcinoma (HPSCC) Arabian Journal of Chemistry Circulating non-coding RNAs hypopharyngeal squamous cell carcinoma (HPSCC) Biomarker Non-invasive diagnosis (NID) |
| title | The diagnostic combination of serum circulating miR-488 and lncRNA AC018761 as biomarkers for hypopharyngeal squamous cell carcinoma (HPSCC) |
| title_full | The diagnostic combination of serum circulating miR-488 and lncRNA AC018761 as biomarkers for hypopharyngeal squamous cell carcinoma (HPSCC) |
| title_fullStr | The diagnostic combination of serum circulating miR-488 and lncRNA AC018761 as biomarkers for hypopharyngeal squamous cell carcinoma (HPSCC) |
| title_full_unstemmed | The diagnostic combination of serum circulating miR-488 and lncRNA AC018761 as biomarkers for hypopharyngeal squamous cell carcinoma (HPSCC) |
| title_short | The diagnostic combination of serum circulating miR-488 and lncRNA AC018761 as biomarkers for hypopharyngeal squamous cell carcinoma (HPSCC) |
| title_sort | diagnostic combination of serum circulating mir 488 and lncrna ac018761 as biomarkers for hypopharyngeal squamous cell carcinoma hpscc |
| topic | Circulating non-coding RNAs hypopharyngeal squamous cell carcinoma (HPSCC) Biomarker Non-invasive diagnosis (NID) |
| url | http://www.sciencedirect.com/science/article/pii/S1878535223003714 |
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