Microfluidic Device for Microinjection of <i>Caenorhabditis elegans</i>
Microinjection is an established and reliable method to deliver transgenic constructs and other reagents to specific locations in <i>C. elegans</i> worms. Specifically, microinjection of a desired DNA construct into the distal gonad is the most widely used method to generate germ-line tr...
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| Format: | Article |
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MDPI AG
2020-03-01
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| Series: | Micromachines |
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| Online Access: | https://www.mdpi.com/2072-666X/11/3/295 |
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| author | Reza Ghaemi Justin Tong Bhagwati P. Gupta P. Ravi Selvaganapathy |
| author_facet | Reza Ghaemi Justin Tong Bhagwati P. Gupta P. Ravi Selvaganapathy |
| author_sort | Reza Ghaemi |
| collection | DOAJ |
| description | Microinjection is an established and reliable method to deliver transgenic constructs and other reagents to specific locations in <i>C. elegans</i> worms. Specifically, microinjection of a desired DNA construct into the distal gonad is the most widely used method to generate germ-line transformation of <i>C. elegans</i>. Although, current <i>C. elegans</i> microinjection method is effective to produce transgenic worms, it requires expensive multi degree of freedom (DOF) micromanipulator, careful injection alignment procedure and skilled operator, all of which make it slow and not suitable for scaling to high throughput. A few microfabricated microinjectors have been developed recently to address these issues. However, none of them are capable of immobilizing a freely mobile animal such as <i>C. elegans</i> worm using a passive immobilization mechanism. Here, a microfluidic microinjector was developed to passively immobilize a freely mobile animal such as <i>C. elegans</i> and simultaneously perform microinjection by using a simple and fast mechanism for needle actuation. The entire process of the microinjection takes ~30 s which includes 10 s for worm loading and aligning, 5 s needle penetration, 5 s reagent injection and 5 s worm unloading. The device is suitable for high-throughput and can be potentially used for creating transgenic <i>C. elegans</i>. |
| first_indexed | 2024-12-22T03:17:20Z |
| format | Article |
| id | doaj.art-a91f62c488b7446083acbba12d76eafb |
| institution | Directory Open Access Journal |
| issn | 2072-666X |
| language | English |
| last_indexed | 2024-12-22T03:17:20Z |
| publishDate | 2020-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Micromachines |
| spelling | doaj.art-a91f62c488b7446083acbba12d76eafb2022-12-21T18:40:48ZengMDPI AGMicromachines2072-666X2020-03-0111329510.3390/mi11030295mi11030295Microfluidic Device for Microinjection of <i>Caenorhabditis elegans</i>Reza Ghaemi0Justin Tong1Bhagwati P. Gupta2P. Ravi Selvaganapathy3Department of Mechanical Engineering, McMaster University, Hamilton, ON L8S 4L8, CanadaDepartment of Biology, McMaster University, Hamilton, ON L8S 4L8, CanadaDepartment of Biology, McMaster University, Hamilton, ON L8S 4L8, CanadaDepartment of Mechanical Engineering, McMaster University, Hamilton, ON L8S 4L8, CanadaMicroinjection is an established and reliable method to deliver transgenic constructs and other reagents to specific locations in <i>C. elegans</i> worms. Specifically, microinjection of a desired DNA construct into the distal gonad is the most widely used method to generate germ-line transformation of <i>C. elegans</i>. Although, current <i>C. elegans</i> microinjection method is effective to produce transgenic worms, it requires expensive multi degree of freedom (DOF) micromanipulator, careful injection alignment procedure and skilled operator, all of which make it slow and not suitable for scaling to high throughput. A few microfabricated microinjectors have been developed recently to address these issues. However, none of them are capable of immobilizing a freely mobile animal such as <i>C. elegans</i> worm using a passive immobilization mechanism. Here, a microfluidic microinjector was developed to passively immobilize a freely mobile animal such as <i>C. elegans</i> and simultaneously perform microinjection by using a simple and fast mechanism for needle actuation. The entire process of the microinjection takes ~30 s which includes 10 s for worm loading and aligning, 5 s needle penetration, 5 s reagent injection and 5 s worm unloading. The device is suitable for high-throughput and can be potentially used for creating transgenic <i>C. elegans</i>.https://www.mdpi.com/2072-666X/11/3/295<i>c. elegans</i>microinjectionmicrofluidicscompliant mechanism |
| spellingShingle | Reza Ghaemi Justin Tong Bhagwati P. Gupta P. Ravi Selvaganapathy Microfluidic Device for Microinjection of <i>Caenorhabditis elegans</i> Micromachines <i>c. elegans</i> microinjection microfluidics compliant mechanism |
| title | Microfluidic Device for Microinjection of <i>Caenorhabditis elegans</i> |
| title_full | Microfluidic Device for Microinjection of <i>Caenorhabditis elegans</i> |
| title_fullStr | Microfluidic Device for Microinjection of <i>Caenorhabditis elegans</i> |
| title_full_unstemmed | Microfluidic Device for Microinjection of <i>Caenorhabditis elegans</i> |
| title_short | Microfluidic Device for Microinjection of <i>Caenorhabditis elegans</i> |
| title_sort | microfluidic device for microinjection of i caenorhabditis elegans i |
| topic | <i>c. elegans</i> microinjection microfluidics compliant mechanism |
| url | https://www.mdpi.com/2072-666X/11/3/295 |
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