Genomic approach to study floral development genes in Rosa sp.
Cultivated for centuries, the varieties of rose have been selected based on a number of flower traits. Understanding the genetic and molecular basis that contributes to these traits will impact on future improvements for this economically important ornamental plant. In this study, we used scanning e...
| Main Authors: | , , , , , , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2011-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC3237435?pdf=render |
| _version_ | 1818528523180048384 |
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| author | Annick Dubois Arnaud Remay Olivier Raymond Sandrine Balzergue Aurélie Chauvet Marion Maene Yann Pécrix Shu-Hua Yang Julien Jeauffre Tatiana Thouroude Véronique Boltz Marie-Laure Martin-Magniette Stéphane Janczarski Fabrice Legeai Jean-Pierre Renou Philippe Vergne Manuel Le Bris Fabrice Foucher Mohammed Bendahmane |
| author_facet | Annick Dubois Arnaud Remay Olivier Raymond Sandrine Balzergue Aurélie Chauvet Marion Maene Yann Pécrix Shu-Hua Yang Julien Jeauffre Tatiana Thouroude Véronique Boltz Marie-Laure Martin-Magniette Stéphane Janczarski Fabrice Legeai Jean-Pierre Renou Philippe Vergne Manuel Le Bris Fabrice Foucher Mohammed Bendahmane |
| author_sort | Annick Dubois |
| collection | DOAJ |
| description | Cultivated for centuries, the varieties of rose have been selected based on a number of flower traits. Understanding the genetic and molecular basis that contributes to these traits will impact on future improvements for this economically important ornamental plant. In this study, we used scanning electron microscopy and sections of meristems and flowers to establish a precise morphological calendar from early rose flower development stages to senescing flowers. Global gene expression was investigated from floral meristem initiation up to flower senescence in three rose genotypes exhibiting contrasted floral traits including continuous versus once flowering and simple versus double flower architecture, using a newly developed Affymetrix microarray (Rosa1_Affyarray) tool containing sequences representing 4765 unigenes expressed during flower development. Data analyses permitted the identification of genes associated with floral transition, floral organs initiation up to flower senescence. Quantitative real time PCR analyses validated the mRNA accumulation changes observed in microarray hybridizations for a selection of 24 genes expressed at either high or low levels. Our data describe the early flower development stages in Rosa sp, the production of a rose microarray and demonstrate its usefulness and reliability to study gene expression during extensive development phases, from the vegetative meristem to the senescent flower. |
| first_indexed | 2024-12-11T06:50:52Z |
| format | Article |
| id | doaj.art-a9202c5287a0458e9bb8cc2aa52f311b |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-11T06:50:52Z |
| publishDate | 2011-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-a9202c5287a0458e9bb8cc2aa52f311b2022-12-22T01:16:55ZengPublic Library of Science (PLoS)PLoS ONE1932-62032011-01-01612e2845510.1371/journal.pone.0028455Genomic approach to study floral development genes in Rosa sp.Annick DuboisArnaud RemayOlivier RaymondSandrine BalzergueAurélie ChauvetMarion MaeneYann PécrixShu-Hua YangJulien JeauffreTatiana ThouroudeVéronique BoltzMarie-Laure Martin-MagnietteStéphane JanczarskiFabrice LegeaiJean-Pierre RenouPhilippe VergneManuel Le BrisFabrice FoucherMohammed BendahmaneCultivated for centuries, the varieties of rose have been selected based on a number of flower traits. Understanding the genetic and molecular basis that contributes to these traits will impact on future improvements for this economically important ornamental plant. In this study, we used scanning electron microscopy and sections of meristems and flowers to establish a precise morphological calendar from early rose flower development stages to senescing flowers. Global gene expression was investigated from floral meristem initiation up to flower senescence in three rose genotypes exhibiting contrasted floral traits including continuous versus once flowering and simple versus double flower architecture, using a newly developed Affymetrix microarray (Rosa1_Affyarray) tool containing sequences representing 4765 unigenes expressed during flower development. Data analyses permitted the identification of genes associated with floral transition, floral organs initiation up to flower senescence. Quantitative real time PCR analyses validated the mRNA accumulation changes observed in microarray hybridizations for a selection of 24 genes expressed at either high or low levels. Our data describe the early flower development stages in Rosa sp, the production of a rose microarray and demonstrate its usefulness and reliability to study gene expression during extensive development phases, from the vegetative meristem to the senescent flower.http://europepmc.org/articles/PMC3237435?pdf=render |
| spellingShingle | Annick Dubois Arnaud Remay Olivier Raymond Sandrine Balzergue Aurélie Chauvet Marion Maene Yann Pécrix Shu-Hua Yang Julien Jeauffre Tatiana Thouroude Véronique Boltz Marie-Laure Martin-Magniette Stéphane Janczarski Fabrice Legeai Jean-Pierre Renou Philippe Vergne Manuel Le Bris Fabrice Foucher Mohammed Bendahmane Genomic approach to study floral development genes in Rosa sp. PLoS ONE |
| title | Genomic approach to study floral development genes in Rosa sp. |
| title_full | Genomic approach to study floral development genes in Rosa sp. |
| title_fullStr | Genomic approach to study floral development genes in Rosa sp. |
| title_full_unstemmed | Genomic approach to study floral development genes in Rosa sp. |
| title_short | Genomic approach to study floral development genes in Rosa sp. |
| title_sort | genomic approach to study floral development genes in rosa sp |
| url | http://europepmc.org/articles/PMC3237435?pdf=render |
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