Autotrophic vs. Heterotrophic Cultivation of the Marine Diatom <i>Cyclotella cryptica</i> for EPA Production
Recently, the marketable value of ω-3 fatty acid, particularly eicosapentaenoic acid (EPA), increased considering their health effects for human consumption. Microalgae are considered a valuable and “green” source of EPA alternative to fish oils, but considerable efforts are necessary for their expl...
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| Format: | Article |
| Language: | English |
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MDPI AG
2021-06-01
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| Series: | Marine Drugs |
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| Online Access: | https://www.mdpi.com/1660-3397/19/7/355 |
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| author | Adelaide Cupo Simone Landi Salvatore Morra Genoveffa Nuzzo Carmela Gallo Emiliano Manzo Angelo Fontana Giuliana d’Ippolito |
| author_facet | Adelaide Cupo Simone Landi Salvatore Morra Genoveffa Nuzzo Carmela Gallo Emiliano Manzo Angelo Fontana Giuliana d’Ippolito |
| author_sort | Adelaide Cupo |
| collection | DOAJ |
| description | Recently, the marketable value of ω-3 fatty acid, particularly eicosapentaenoic acid (EPA), increased considering their health effects for human consumption. Microalgae are considered a valuable and “green” source of EPA alternative to fish oils, but considerable efforts are necessary for their exploitation at an industrial level. Due to the high operation costs of photoautotrophic microalgae cultivation, heterotrophic growth represents a promising economic solution. Marine diatoms are the major ecological producers of ω-3 fatty acids. Few species of diatoms are capable to grow in the dark using organic carbon sources. The marine diatom <i>Cyclotella cryptica</i> was cultivated for 14 days under photoautotrophic and heterotrophic conditions to define the effects on growth parameters, lipid production, total fatty acids and EPA content. Photoautotrophic conditions led to a total EPA production of 1.6% of dry weight, 12.2 mg L<sup>−1</sup> culture and productivity of 0.9 mg L<sup>−1</sup> day<sup>−1</sup>. The heterotrophy cultures reported a total EPA production of 2.7% of dry cell weight, 18 mg L<sup>−1</sup> culture, a productivity of 1.3 mg L<sup>−1</sup> day<sup>−1</sup>, which are promising values in the prospective of improving culture parameters for the biotechnological exploitation of dark cultivation. <i>C. cryptica</i> could be a potential candidate for the heterotrophic production of EPA, also considering its robustness, capacity to resist to bacterial contaminations and plasticity of lipid metabolism. |
| first_indexed | 2024-03-10T10:09:57Z |
| format | Article |
| id | doaj.art-a921443b24c64614b2a36446eeddc213 |
| institution | Directory Open Access Journal |
| issn | 1660-3397 |
| language | English |
| last_indexed | 2024-03-10T10:09:57Z |
| publishDate | 2021-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Marine Drugs |
| spelling | doaj.art-a921443b24c64614b2a36446eeddc2132023-11-22T01:18:31ZengMDPI AGMarine Drugs1660-33972021-06-0119735510.3390/md19070355Autotrophic vs. Heterotrophic Cultivation of the Marine Diatom <i>Cyclotella cryptica</i> for EPA ProductionAdelaide Cupo0Simone Landi1Salvatore Morra2Genoveffa Nuzzo3Carmela Gallo4Emiliano Manzo5Angelo Fontana6Giuliana d’Ippolito7Institute of Biomolecular Chemistry ICB-CNR, Via Campi Flegrei 34, 80078 Naples, ItalyDepartment of Biology, University of Naples “Federico II”, Via Cinthia, 80126 Napoli, ItalyInstitute of Biomolecular Chemistry ICB-CNR, Via Campi Flegrei 34, 80078 Naples, ItalyInstitute of Biomolecular Chemistry ICB-CNR, Via Campi Flegrei 34, 80078 Naples, ItalyInstitute of Biomolecular Chemistry ICB-CNR, Via Campi Flegrei 34, 80078 Naples, ItalyInstitute of Biomolecular Chemistry ICB-CNR, Via Campi Flegrei 34, 80078 Naples, ItalyInstitute of Biomolecular Chemistry ICB-CNR, Via Campi Flegrei 34, 80078 Naples, ItalyInstitute of Biomolecular Chemistry ICB-CNR, Via Campi Flegrei 34, 80078 Naples, ItalyRecently, the marketable value of ω-3 fatty acid, particularly eicosapentaenoic acid (EPA), increased considering their health effects for human consumption. Microalgae are considered a valuable and “green” source of EPA alternative to fish oils, but considerable efforts are necessary for their exploitation at an industrial level. Due to the high operation costs of photoautotrophic microalgae cultivation, heterotrophic growth represents a promising economic solution. Marine diatoms are the major ecological producers of ω-3 fatty acids. Few species of diatoms are capable to grow in the dark using organic carbon sources. The marine diatom <i>Cyclotella cryptica</i> was cultivated for 14 days under photoautotrophic and heterotrophic conditions to define the effects on growth parameters, lipid production, total fatty acids and EPA content. Photoautotrophic conditions led to a total EPA production of 1.6% of dry weight, 12.2 mg L<sup>−1</sup> culture and productivity of 0.9 mg L<sup>−1</sup> day<sup>−1</sup>. The heterotrophy cultures reported a total EPA production of 2.7% of dry cell weight, 18 mg L<sup>−1</sup> culture, a productivity of 1.3 mg L<sup>−1</sup> day<sup>−1</sup>, which are promising values in the prospective of improving culture parameters for the biotechnological exploitation of dark cultivation. <i>C. cryptica</i> could be a potential candidate for the heterotrophic production of EPA, also considering its robustness, capacity to resist to bacterial contaminations and plasticity of lipid metabolism.https://www.mdpi.com/1660-3397/19/7/355microalgaeomega-3 fatty acidsbiomasslipids |
| spellingShingle | Adelaide Cupo Simone Landi Salvatore Morra Genoveffa Nuzzo Carmela Gallo Emiliano Manzo Angelo Fontana Giuliana d’Ippolito Autotrophic vs. Heterotrophic Cultivation of the Marine Diatom <i>Cyclotella cryptica</i> for EPA Production Marine Drugs microalgae omega-3 fatty acids biomass lipids |
| title | Autotrophic vs. Heterotrophic Cultivation of the Marine Diatom <i>Cyclotella cryptica</i> for EPA Production |
| title_full | Autotrophic vs. Heterotrophic Cultivation of the Marine Diatom <i>Cyclotella cryptica</i> for EPA Production |
| title_fullStr | Autotrophic vs. Heterotrophic Cultivation of the Marine Diatom <i>Cyclotella cryptica</i> for EPA Production |
| title_full_unstemmed | Autotrophic vs. Heterotrophic Cultivation of the Marine Diatom <i>Cyclotella cryptica</i> for EPA Production |
| title_short | Autotrophic vs. Heterotrophic Cultivation of the Marine Diatom <i>Cyclotella cryptica</i> for EPA Production |
| title_sort | autotrophic vs heterotrophic cultivation of the marine diatom i cyclotella cryptica i for epa production |
| topic | microalgae omega-3 fatty acids biomass lipids |
| url | https://www.mdpi.com/1660-3397/19/7/355 |
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