Platelet adhesion on commercially pure titanium plates in vitro III: effects of calcium phosphate-blasting on titanium plate biocompatibility
Abstract Background Platelet-rich plasma (PRP) is often used to improve surface biocompatibility. We previously found that platelets rapidly adhere to plain commercially pure titanium (cp-Ti) plates in the absence, but not in the presence, of plasma proteins. To further expand on these findings, in...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
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SpringerOpen
2020-11-01
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| Series: | International Journal of Implant Dentistry |
| Subjects: | |
| Online Access: | http://link.springer.com/article/10.1186/s40729-020-00270-2 |
| _version_ | 1818116377206063104 |
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| author | Masayuki Nakamura Hachidai Aizawa Hideo Kawabata Atsushi Sato Taisuke Watanabe Kazushige Isobe Yutaka Kitamura Takaaki Tanaka Tomoyuki Kawase |
| author_facet | Masayuki Nakamura Hachidai Aizawa Hideo Kawabata Atsushi Sato Taisuke Watanabe Kazushige Isobe Yutaka Kitamura Takaaki Tanaka Tomoyuki Kawase |
| author_sort | Masayuki Nakamura |
| collection | DOAJ |
| description | Abstract Background Platelet-rich plasma (PRP) is often used to improve surface biocompatibility. We previously found that platelets rapidly adhere to plain commercially pure titanium (cp-Ti) plates in the absence, but not in the presence, of plasma proteins. To further expand on these findings, in the present study, we switched titanium plates from a plain surface to a rough surface that is blasted with calcium phosphate (CaP) powder and then examined platelet adhesion and activation. Methods Elemental distribution in CaP-blasted cp-Ti plates was analyzed using energy-dispersive X-ray spectroscopy. PRP samples prepared from anticoagulated blood samples of six healthy, non-smoking adult male donors were loaded on CaP-blasted cp-Ti plates for 1 h and fixed for examination of platelet morphology and visualization of PDGF-B and platelet surface markers (CD62P, CD63) using scanning electron microscopy and fluorescence microscopy. Plain SUS316L stainless steel plates used in injection needles were also examined for comparison. Results Significant amounts of calcium and phosphate were detected on the CaP-blasted cp-Ti surface. Platelets rapidly adhered to this surface, leading to higher activation. Platelets also adhered to the plain stainless surface; however, the levels of adhesion and activation were much lower than those observed on the CaP-blasted cp-Ti plate. Conclusions The CaP-blasted cp-Ti surface efficiently entraps and activates platelets. Biomolecules released from the activated platelets could be retained by the fibrin matrix on the surface to facilitate regeneration of the surrounding tissues. Thus, PRP immersion could not only eliminate surface air bubbles but also improve the biocompatibility of the implant surface. |
| first_indexed | 2024-12-11T04:21:33Z |
| format | Article |
| id | doaj.art-a922298c3888465c9bec12a65308f0bd |
| institution | Directory Open Access Journal |
| issn | 2198-4034 |
| language | English |
| last_indexed | 2024-12-11T04:21:33Z |
| publishDate | 2020-11-01 |
| publisher | SpringerOpen |
| record_format | Article |
| series | International Journal of Implant Dentistry |
| spelling | doaj.art-a922298c3888465c9bec12a65308f0bd2022-12-22T01:21:06ZengSpringerOpenInternational Journal of Implant Dentistry2198-40342020-11-016111410.1186/s40729-020-00270-2Platelet adhesion on commercially pure titanium plates in vitro III: effects of calcium phosphate-blasting on titanium plate biocompatibilityMasayuki Nakamura0Hachidai Aizawa1Hideo Kawabata2Atsushi Sato3Taisuke Watanabe4Kazushige Isobe5Yutaka Kitamura6Takaaki Tanaka7Tomoyuki Kawase8Tokyo Plastic Dental SocietyTokyo Plastic Dental SocietyTokyo Plastic Dental SocietyTokyo Plastic Dental SocietyTokyo Plastic Dental SocietyTokyo Plastic Dental SocietyTokyo Plastic Dental SocietyDepartment of Materials Science and Technology, Niigata UniversityDivision of Oral Bioengineering, Institute of Medicine and Dentistry, Niigata UniversityAbstract Background Platelet-rich plasma (PRP) is often used to improve surface biocompatibility. We previously found that platelets rapidly adhere to plain commercially pure titanium (cp-Ti) plates in the absence, but not in the presence, of plasma proteins. To further expand on these findings, in the present study, we switched titanium plates from a plain surface to a rough surface that is blasted with calcium phosphate (CaP) powder and then examined platelet adhesion and activation. Methods Elemental distribution in CaP-blasted cp-Ti plates was analyzed using energy-dispersive X-ray spectroscopy. PRP samples prepared from anticoagulated blood samples of six healthy, non-smoking adult male donors were loaded on CaP-blasted cp-Ti plates for 1 h and fixed for examination of platelet morphology and visualization of PDGF-B and platelet surface markers (CD62P, CD63) using scanning electron microscopy and fluorescence microscopy. Plain SUS316L stainless steel plates used in injection needles were also examined for comparison. Results Significant amounts of calcium and phosphate were detected on the CaP-blasted cp-Ti surface. Platelets rapidly adhered to this surface, leading to higher activation. Platelets also adhered to the plain stainless surface; however, the levels of adhesion and activation were much lower than those observed on the CaP-blasted cp-Ti plate. Conclusions The CaP-blasted cp-Ti surface efficiently entraps and activates platelets. Biomolecules released from the activated platelets could be retained by the fibrin matrix on the surface to facilitate regeneration of the surrounding tissues. Thus, PRP immersion could not only eliminate surface air bubbles but also improve the biocompatibility of the implant surface.http://link.springer.com/article/10.1186/s40729-020-00270-2Platelet-rich plasmaCD62PCD63TitaniumImplantCalcium phosphates |
| spellingShingle | Masayuki Nakamura Hachidai Aizawa Hideo Kawabata Atsushi Sato Taisuke Watanabe Kazushige Isobe Yutaka Kitamura Takaaki Tanaka Tomoyuki Kawase Platelet adhesion on commercially pure titanium plates in vitro III: effects of calcium phosphate-blasting on titanium plate biocompatibility International Journal of Implant Dentistry Platelet-rich plasma CD62P CD63 Titanium Implant Calcium phosphates |
| title | Platelet adhesion on commercially pure titanium plates in vitro III: effects of calcium phosphate-blasting on titanium plate biocompatibility |
| title_full | Platelet adhesion on commercially pure titanium plates in vitro III: effects of calcium phosphate-blasting on titanium plate biocompatibility |
| title_fullStr | Platelet adhesion on commercially pure titanium plates in vitro III: effects of calcium phosphate-blasting on titanium plate biocompatibility |
| title_full_unstemmed | Platelet adhesion on commercially pure titanium plates in vitro III: effects of calcium phosphate-blasting on titanium plate biocompatibility |
| title_short | Platelet adhesion on commercially pure titanium plates in vitro III: effects of calcium phosphate-blasting on titanium plate biocompatibility |
| title_sort | platelet adhesion on commercially pure titanium plates in vitro iii effects of calcium phosphate blasting on titanium plate biocompatibility |
| topic | Platelet-rich plasma CD62P CD63 Titanium Implant Calcium phosphates |
| url | http://link.springer.com/article/10.1186/s40729-020-00270-2 |
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