Assessment of genetic diversity among surian Toona sinensis Roem in progenies test using random amplified polymorphic DNA markers
Surian Toona sinensis Roem is one of the most widely planted species in Indonesia. This study aimed to estimate the genetic diversity between a number of surian populations in a progeny test using RAPD markers, with the goal of proposing management strategies for a surian breeding program. Ninety-si...
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| Format: | Article |
| Language: | English |
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Universitas Gadjah Mada, Yogyakarta
2017-06-01
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| Series: | Indonesian Journal of Biotechnology |
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| Online Access: | https://jurnal.ugm.ac.id/ijbiotech/article/view/25798 |
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| author | Jayusman Jayusman Muhammad Na’iem Sapto Indrioko Eko Bhakti Hardiyanto ILG Nurcahyaningsih |
| author_facet | Jayusman Jayusman Muhammad Na’iem Sapto Indrioko Eko Bhakti Hardiyanto ILG Nurcahyaningsih |
| author_sort | Jayusman Jayusman |
| collection | DOAJ |
| description | Surian Toona sinensis Roem is one of the most widely planted species in Indonesia. This study aimed to estimate the genetic diversity between a number of surian populations in a progeny test using RAPD markers, with the goal of proposing management strategies for a surian breeding program. Ninety-six individual trees from 8 populations of surian were chosen as samples for analysis. Eleven polymorphic primers (OP-B3, OP-B4, OP-B10, OP-H3, OP-Y6, OP-Y7, OP-Y8, OP-Y10, OP-Y11, OP-Y14, and OP-06) producing reproducible bands were analyzed for the 96 trees, with six trees per family sampled. Data were analyzed using GenAlEx 6.3, NTSYS 2.02. The observed percentage of polymorphic loci ranged from 18.2% to 50%. The mean level of genetic diversity among the surian populations was considered to be moderate (He 0.304). Cluster analysis grouped the genotypes into two main clusters, at similarity levels of 0.68 and 0.46. The first two axes of the PCoA explained 46.16% and 25.54% of the total variation, respectively. The grouping of samples into clusters and subclusters did not correspond with family and their distances, but the grouping was in line with the genetic distances of the samples. |
| first_indexed | 2024-04-11T22:49:54Z |
| format | Article |
| id | doaj.art-a923a5d467654217adcb22dc2692b3d1 |
| institution | Directory Open Access Journal |
| issn | 0853-8654 2089-2241 |
| language | English |
| last_indexed | 2024-04-11T22:49:54Z |
| publishDate | 2017-06-01 |
| publisher | Universitas Gadjah Mada, Yogyakarta |
| record_format | Article |
| series | Indonesian Journal of Biotechnology |
| spelling | doaj.art-a923a5d467654217adcb22dc2692b3d12022-12-22T03:58:37ZengUniversitas Gadjah Mada, YogyakartaIndonesian Journal of Biotechnology0853-86542089-22412017-06-01221223010.22146/ijbiotech.2579819164Assessment of genetic diversity among surian Toona sinensis Roem in progenies test using random amplified polymorphic DNA markersJayusman Jayusman0Muhammad Na’iemSapto Indrioko1Eko Bhakti Hardiyanto2ILG Nurcahyaningsih3Centre for Forest Biotechnology and Tree Improvement Research and Development, Jalan Palagan Tentara Pelajar Km. 15, Purwobinangun, Pakem, Sleman, Yogyakarta 55582, IndonesiaFaculty of Forestry, Universitas Gadjah Mada, Jalan Agro No. 1, Bulaksumur, Yogyakarta 55281, IndonesiaFaculty of Forestry, Universitas Gadjah Mada, Jalan Agro No. 1, Bulaksumur, Yogyakarta 55281, IndonesiaCentre for Forest Biotechnology and Tree Improvement Research and Development, Jalan Palagan Tentara Pelajar Km. 15, Purwobinangun, Pakem, Sleman, Yogyakarta 55582, IndonesiaSurian Toona sinensis Roem is one of the most widely planted species in Indonesia. This study aimed to estimate the genetic diversity between a number of surian populations in a progeny test using RAPD markers, with the goal of proposing management strategies for a surian breeding program. Ninety-six individual trees from 8 populations of surian were chosen as samples for analysis. Eleven polymorphic primers (OP-B3, OP-B4, OP-B10, OP-H3, OP-Y6, OP-Y7, OP-Y8, OP-Y10, OP-Y11, OP-Y14, and OP-06) producing reproducible bands were analyzed for the 96 trees, with six trees per family sampled. Data were analyzed using GenAlEx 6.3, NTSYS 2.02. The observed percentage of polymorphic loci ranged from 18.2% to 50%. The mean level of genetic diversity among the surian populations was considered to be moderate (He 0.304). Cluster analysis grouped the genotypes into two main clusters, at similarity levels of 0.68 and 0.46. The first two axes of the PCoA explained 46.16% and 25.54% of the total variation, respectively. The grouping of samples into clusters and subclusters did not correspond with family and their distances, but the grouping was in line with the genetic distances of the samples.https://jurnal.ugm.ac.id/ijbiotech/article/view/25798genetic diversityprogeny testRAPDToona sinensis |
| spellingShingle | Jayusman Jayusman Muhammad Na’iem Sapto Indrioko Eko Bhakti Hardiyanto ILG Nurcahyaningsih Assessment of genetic diversity among surian Toona sinensis Roem in progenies test using random amplified polymorphic DNA markers Indonesian Journal of Biotechnology genetic diversity progeny test RAPD Toona sinensis |
| title | Assessment of genetic diversity among surian Toona sinensis Roem in progenies test using random amplified polymorphic DNA markers |
| title_full | Assessment of genetic diversity among surian Toona sinensis Roem in progenies test using random amplified polymorphic DNA markers |
| title_fullStr | Assessment of genetic diversity among surian Toona sinensis Roem in progenies test using random amplified polymorphic DNA markers |
| title_full_unstemmed | Assessment of genetic diversity among surian Toona sinensis Roem in progenies test using random amplified polymorphic DNA markers |
| title_short | Assessment of genetic diversity among surian Toona sinensis Roem in progenies test using random amplified polymorphic DNA markers |
| title_sort | assessment of genetic diversity among surian toona sinensis roem in progenies test using random amplified polymorphic dna markers |
| topic | genetic diversity progeny test RAPD Toona sinensis |
| url | https://jurnal.ugm.ac.id/ijbiotech/article/view/25798 |
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