Biochanin A Regulates Cholesterol Metabolism Further Delays the Progression of Nonalcoholic Fatty Liver Disease
Yan Fan,1,* Long-Teng Yan,2,* Zheng Yao,2 Guang-Yi Xiong2 1Department of Anatomy, Histology, and Embryology, School of Basic Medical Science, Kunming Medical University, Kunming, Yunnan, 650500, People’s Republic of China; 2Key Laboratory of Microcosmic Syndrome Differentiation, Scho...
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Dove Medical Press
2021-07-01
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| Series: | Diabetes, Metabolic Syndrome and Obesity |
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| Online Access: | https://www.dovepress.com/biochanin-a-regulates-cholesterol-metabolism-further-delays-the-progre-peer-reviewed-fulltext-article-DMSO |
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| author | Fan Y Yan LT Yao Z Xiong GY |
| author_facet | Fan Y Yan LT Yao Z Xiong GY |
| author_sort | Fan Y |
| collection | DOAJ |
| description | Yan Fan,1,* Long-Teng Yan,2,* Zheng Yao,2 Guang-Yi Xiong2 1Department of Anatomy, Histology, and Embryology, School of Basic Medical Science, Kunming Medical University, Kunming, Yunnan, 650500, People’s Republic of China; 2Key Laboratory of Microcosmic Syndrome Differentiation, School of Basic Medicine, Yunnan University of Chinese Medicine, Kunming, Yunnan, 650500, People’s Republic of China*These authors contributed equally to this workCorrespondence: Zheng Yao; Guang-Yi XiongKey Laboratory of Microcosmic Syndrome Differentiation, School of Basic Medicine, Yunnan University of Chinese Medicine, No. 1076, Yuhua Road, Chenggong District, Kunming, Yunnan, 650500, People’s Republic of ChinaTel/Fax +86 189 0871 9365Email zhengyaomail@126.com; 307924002@qq.comPurpose: To discover the possible target of biochanin A (BCA) in the lipid metabolism pathway and further explore its mechanism to nonalcoholic fatty liver disease (NAFLD).Methods: We adopted a high-fat and high-glucose diet for 12 weeks to build the NAFLD rat model, which was then treated with different proportions of BCA for 4 weeks. General condition, body weight, Lee index, and liver index were then evaluated. Furthermore, blood lipid level and insulin resistance (IR) were detected. Moreover, hematoxylin and eosin and oil red O staining were used to observe the pathological changes in the liver. Finally, Western blotting was used to detect the protein expression levels of CYP7A1, HMGCR, LDLR, PPAR-α, PPAR-γ, and SREBP-1c in the liver.Results: The vital signs of rats in each group were stable. The treatment with BCA effectively reduced Lee index and liver index (F = 104.781, P < 0.05); however, the weight was not effected in each group. Additionally, BCA effectively reduced the related lipid metabolism indexes of NAFLD, such as total cholesterol (TC), triglyceride (TG), low-density lipoprotein (LDL), blood glucose, insulin, IR (F =12.463 (TC), 6.909 [TG], and 15.3 effected 75 [LDL], P < 0.05), and increased HDL (F = 11.580, P < 0.05). We observed that BCA could significantly improve steatosis and inflammatory cell infiltration in liver slices. Furthermore, BCA significantly increased the CYP7A1, LDLR, and PPAR-α protein expression in the liver and downregulated the HMGCR, SREBP-1c, and PPAR-γ protein expression.Conclusion: BCA could delay the liver damage of NAFLD induced by a high-fat diet, regulate the blood lipid level, and improve the expression of lipid metabolism-related genes in rats.Keywords: biochanin A, nonalcoholic fatty liver disease, NAFLD, cholesterol metabolism |
| first_indexed | 2024-04-10T18:01:53Z |
| format | Article |
| id | doaj.art-a9279746a3b04a009d334bc7b721ceba |
| institution | Directory Open Access Journal |
| issn | 1178-7007 |
| language | English |
| last_indexed | 2024-04-10T18:01:53Z |
| publishDate | 2021-07-01 |
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| series | Diabetes, Metabolic Syndrome and Obesity |
| spelling | doaj.art-a9279746a3b04a009d334bc7b721ceba2023-02-02T15:11:23ZengDove Medical PressDiabetes, Metabolic Syndrome and Obesity1178-70072021-07-01Volume 143161317266802Biochanin A Regulates Cholesterol Metabolism Further Delays the Progression of Nonalcoholic Fatty Liver DiseaseFan YYan LTYao ZXiong GYYan Fan,1,* Long-Teng Yan,2,* Zheng Yao,2 Guang-Yi Xiong2 1Department of Anatomy, Histology, and Embryology, School of Basic Medical Science, Kunming Medical University, Kunming, Yunnan, 650500, People’s Republic of China; 2Key Laboratory of Microcosmic Syndrome Differentiation, School of Basic Medicine, Yunnan University of Chinese Medicine, Kunming, Yunnan, 650500, People’s Republic of China*These authors contributed equally to this workCorrespondence: Zheng Yao; Guang-Yi XiongKey Laboratory of Microcosmic Syndrome Differentiation, School of Basic Medicine, Yunnan University of Chinese Medicine, No. 1076, Yuhua Road, Chenggong District, Kunming, Yunnan, 650500, People’s Republic of ChinaTel/Fax +86 189 0871 9365Email zhengyaomail@126.com; 307924002@qq.comPurpose: To discover the possible target of biochanin A (BCA) in the lipid metabolism pathway and further explore its mechanism to nonalcoholic fatty liver disease (NAFLD).Methods: We adopted a high-fat and high-glucose diet for 12 weeks to build the NAFLD rat model, which was then treated with different proportions of BCA for 4 weeks. General condition, body weight, Lee index, and liver index were then evaluated. Furthermore, blood lipid level and insulin resistance (IR) were detected. Moreover, hematoxylin and eosin and oil red O staining were used to observe the pathological changes in the liver. Finally, Western blotting was used to detect the protein expression levels of CYP7A1, HMGCR, LDLR, PPAR-α, PPAR-γ, and SREBP-1c in the liver.Results: The vital signs of rats in each group were stable. The treatment with BCA effectively reduced Lee index and liver index (F = 104.781, P < 0.05); however, the weight was not effected in each group. Additionally, BCA effectively reduced the related lipid metabolism indexes of NAFLD, such as total cholesterol (TC), triglyceride (TG), low-density lipoprotein (LDL), blood glucose, insulin, IR (F =12.463 (TC), 6.909 [TG], and 15.3 effected 75 [LDL], P < 0.05), and increased HDL (F = 11.580, P < 0.05). We observed that BCA could significantly improve steatosis and inflammatory cell infiltration in liver slices. Furthermore, BCA significantly increased the CYP7A1, LDLR, and PPAR-α protein expression in the liver and downregulated the HMGCR, SREBP-1c, and PPAR-γ protein expression.Conclusion: BCA could delay the liver damage of NAFLD induced by a high-fat diet, regulate the blood lipid level, and improve the expression of lipid metabolism-related genes in rats.Keywords: biochanin A, nonalcoholic fatty liver disease, NAFLD, cholesterol metabolismhttps://www.dovepress.com/biochanin-a-regulates-cholesterol-metabolism-further-delays-the-progre-peer-reviewed-fulltext-article-DMSObiochanin a；nonalcoholic fatty liver diseasenafldcholesterol metabolism |
| spellingShingle | Fan Y Yan LT Yao Z Xiong GY Biochanin A Regulates Cholesterol Metabolism Further Delays the Progression of Nonalcoholic Fatty Liver Disease Diabetes, Metabolic Syndrome and Obesity biochanin a；nonalcoholic fatty liver disease nafld cholesterol metabolism |
| title | Biochanin A Regulates Cholesterol Metabolism Further Delays the Progression of Nonalcoholic Fatty Liver Disease |
| title_full | Biochanin A Regulates Cholesterol Metabolism Further Delays the Progression of Nonalcoholic Fatty Liver Disease |
| title_fullStr | Biochanin A Regulates Cholesterol Metabolism Further Delays the Progression of Nonalcoholic Fatty Liver Disease |
| title_full_unstemmed | Biochanin A Regulates Cholesterol Metabolism Further Delays the Progression of Nonalcoholic Fatty Liver Disease |
| title_short | Biochanin A Regulates Cholesterol Metabolism Further Delays the Progression of Nonalcoholic Fatty Liver Disease |
| title_sort | biochanin a regulates cholesterol metabolism further delays the progression of nonalcoholic fatty liver disease |
| topic | biochanin a；nonalcoholic fatty liver disease nafld cholesterol metabolism |
| url | https://www.dovepress.com/biochanin-a-regulates-cholesterol-metabolism-further-delays-the-progre-peer-reviewed-fulltext-article-DMSO |
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