Effects of lactic acid bacteria isolated from fermented mustard on lowering cholesterol
Objective: To evaluate the ability of lactic acid bacteria (LAB) strains isolated from fermented mustard to lower the cholesterol in vitro. Methods: The ability of 50 LAB strains isolated from fermented mustard on lowering cholesterol in vitro was determined by modified o-phtshalaldehyde method. The...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Wolters Kluwer Medknow Publications
2014-07-01
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| Series: | Asian Pacific Journal of Tropical Biomedicine |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2221169115301234 |
| _version_ | 1818608124728180736 |
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| author | Shu Chen Wang Chen Kai Chang Shu Chang Chan Jiunn Shiuh Shieh Chih Kwang Chiu Pin-Der Duh |
| author_facet | Shu Chen Wang Chen Kai Chang Shu Chang Chan Jiunn Shiuh Shieh Chih Kwang Chiu Pin-Der Duh |
| author_sort | Shu Chen Wang |
| collection | DOAJ |
| description | Objective: To evaluate the ability of lactic acid bacteria (LAB) strains isolated from fermented mustard to lower the cholesterol in vitro.
Methods: The ability of 50 LAB strains isolated from fermented mustard on lowering cholesterol in vitro was determined by modified o-phtshalaldehyde method. The LAB isolates were analyzed for their resistance to acid and bile salt. Strains with lowering cholesterol activity, were determined adherence to Caco-2 cells.
Results: Strain B0007, B0006 and B0022 assimilated more cholesterol than BCRC10474 and BCRC 17010. The isolated strains showed tolerance to pH 3.0 for 3 h despite variations in the degree of viability and bile-tolerant strains, with more than 108 CFU/mL after incubation for 24 h at 1% oxigall in MRS. In addition, strain B0007 and B0022 identified as Lactobacillus plantarum with 16S rDNA sequences were able to adhere to the Caco-2 cell lines.
Conclusions: These strains B0007 and B0022 may be potential functional sources for cholesterol-lowering activities as well as adhering to Caco-2 cell lines. |
| first_indexed | 2024-12-16T14:37:40Z |
| format | Article |
| id | doaj.art-a92c229c653b4526bc6172fd4219c494 |
| institution | Directory Open Access Journal |
| issn | 2221-1691 |
| language | English |
| last_indexed | 2024-12-16T14:37:40Z |
| publishDate | 2014-07-01 |
| publisher | Wolters Kluwer Medknow Publications |
| record_format | Article |
| series | Asian Pacific Journal of Tropical Biomedicine |
| spelling | doaj.art-a92c229c653b4526bc6172fd4219c4942022-12-21T22:28:03ZengWolters Kluwer Medknow PublicationsAsian Pacific Journal of Tropical Biomedicine2221-16912014-07-014752352810.12980/APJTB.4.201414B54Effects of lactic acid bacteria isolated from fermented mustard on lowering cholesterolShu Chen Wang0Chen Kai Chang1Shu Chang Chan2Jiunn Shiuh Shieh3Chih Kwang Chiu4Pin-Der Duh5Department of Food Science and Technology, Chia Nan University of Pharmacy and Science, 60 Erh-Jen Road, Section 1, Pao-An, Jen-Te District, Tainan, TaiwanDepartment of Food Science and Technology, Chia Nan University of Pharmacy and Science, 60 Erh-Jen Road, Section 1, Pao-An, Jen-Te District, Tainan, TaiwanDepartment of Biotechnology, Chia Nan University of Pharmacy and Science, 60 Erh-Jen Road, Section 1, Pao-An, Jen-Te District, Tainan, TaiwanDepartment of Food Science and Technology, Chia Nan University of Pharmacy and Science, 60 Erh-Jen Road, Section 1, Pao-An, Jen-Te District, Tainan, TaiwanDepartment of Food Science and Technology, Chia Nan University of Pharmacy and Science, 60 Erh-Jen Road, Section 1, Pao-An, Jen-Te District, Tainan, TaiwanDepartment of Food Science and Technology, Chia Nan University of Pharmacy and Science, 60 Erh-Jen Road, Section 1, Pao-An, Jen-Te District, Tainan, TaiwanObjective: To evaluate the ability of lactic acid bacteria (LAB) strains isolated from fermented mustard to lower the cholesterol in vitro. Methods: The ability of 50 LAB strains isolated from fermented mustard on lowering cholesterol in vitro was determined by modified o-phtshalaldehyde method. The LAB isolates were analyzed for their resistance to acid and bile salt. Strains with lowering cholesterol activity, were determined adherence to Caco-2 cells. Results: Strain B0007, B0006 and B0022 assimilated more cholesterol than BCRC10474 and BCRC 17010. The isolated strains showed tolerance to pH 3.0 for 3 h despite variations in the degree of viability and bile-tolerant strains, with more than 108 CFU/mL after incubation for 24 h at 1% oxigall in MRS. In addition, strain B0007 and B0022 identified as Lactobacillus plantarum with 16S rDNA sequences were able to adhere to the Caco-2 cell lines. Conclusions: These strains B0007 and B0022 may be potential functional sources for cholesterol-lowering activities as well as adhering to Caco-2 cell lines.http://www.sciencedirect.com/science/article/pii/S2221169115301234Cholesterol-lowering activityProbioticLactic acid bacteriaAcidBile tolerance |
| spellingShingle | Shu Chen Wang Chen Kai Chang Shu Chang Chan Jiunn Shiuh Shieh Chih Kwang Chiu Pin-Der Duh Effects of lactic acid bacteria isolated from fermented mustard on lowering cholesterol Asian Pacific Journal of Tropical Biomedicine Cholesterol-lowering activity Probiotic Lactic acid bacteria Acid Bile tolerance |
| title | Effects of lactic acid bacteria isolated from fermented mustard on lowering cholesterol |
| title_full | Effects of lactic acid bacteria isolated from fermented mustard on lowering cholesterol |
| title_fullStr | Effects of lactic acid bacteria isolated from fermented mustard on lowering cholesterol |
| title_full_unstemmed | Effects of lactic acid bacteria isolated from fermented mustard on lowering cholesterol |
| title_short | Effects of lactic acid bacteria isolated from fermented mustard on lowering cholesterol |
| title_sort | effects of lactic acid bacteria isolated from fermented mustard on lowering cholesterol |
| topic | Cholesterol-lowering activity Probiotic Lactic acid bacteria Acid Bile tolerance |
| url | http://www.sciencedirect.com/science/article/pii/S2221169115301234 |
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