Uncovering Phytotoxic Compounds Produced by <i>Colletotrichum</i> spp. Involved in Legume Diseases Using an OSMAC–Metabolomics Approach
Different fungal species belonging to the <i>Colletotrichum</i> genus cause anthracnose disease in a range of major crops, resulting in huge economic losses worldwide. Typical symptoms include dark, sunken lesions on leaves, stems, or fruits. <i>Colletotrichum</i> spp. have s...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2023-05-01
|
| Series: | Journal of Fungi |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2309-608X/9/6/610 |
| _version_ | 1797594015346982912 |
|---|---|
| author | Pierluigi Reveglia Francisco J. Agudo-Jurado Eleonora Barilli Marco Masi Antonio Evidente Diego Rubiales |
| author_facet | Pierluigi Reveglia Francisco J. Agudo-Jurado Eleonora Barilli Marco Masi Antonio Evidente Diego Rubiales |
| author_sort | Pierluigi Reveglia |
| collection | DOAJ |
| description | Different fungal species belonging to the <i>Colletotrichum</i> genus cause anthracnose disease in a range of major crops, resulting in huge economic losses worldwide. Typical symptoms include dark, sunken lesions on leaves, stems, or fruits. <i>Colletotrichum</i> spp. have synthesized, in vitro, a number of biologically active and structurally unusual metabolites that are involved in their host’s infection process. In this study, we applied a one strain many compounds (OSMAC) approach, integrated with targeted and non-targeted metabolomics profiling, to shed light on the secondary phytotoxic metabolite panels produced by pathogenic isolates of <i>Colletotrichum truncatum</i> and <i>Colletotrichum trifolii</i>. The phytotoxicity of the fungal crude extracts was also assessed on their primary hosts and related legumes, and the results correlated with the metabolite profile that arose from the different cultural conditions. To the best of our knowledge, this is the first time that the OSMAC strategy integrated with metabolomics approaches has been applied to <i>Colletotrichum</i> species involved in legume diseases. |
| first_indexed | 2024-03-11T02:16:37Z |
| format | Article |
| id | doaj.art-a92de25a76ad4803a2b60204f8a430eb |
| institution | Directory Open Access Journal |
| issn | 2309-608X |
| language | English |
| last_indexed | 2024-03-11T02:16:37Z |
| publishDate | 2023-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Journal of Fungi |
| spelling | doaj.art-a92de25a76ad4803a2b60204f8a430eb2023-11-18T11:08:21ZengMDPI AGJournal of Fungi2309-608X2023-05-019661010.3390/jof9060610Uncovering Phytotoxic Compounds Produced by <i>Colletotrichum</i> spp. Involved in Legume Diseases Using an OSMAC–Metabolomics ApproachPierluigi Reveglia0Francisco J. Agudo-Jurado1Eleonora Barilli2Marco Masi3Antonio Evidente4Diego Rubiales5Institute for Sustainable Agriculture, CSIC, 14004 Cordoba, SpainInstitute for Sustainable Agriculture, CSIC, 14004 Cordoba, SpainInstitute for Sustainable Agriculture, CSIC, 14004 Cordoba, SpainDepartment of Chemical Sciences, University of Naples Federico II (UNINA), 80126 Naples, ItalyDepartment of Chemical Sciences, University of Naples Federico II (UNINA), 80126 Naples, ItalyInstitute for Sustainable Agriculture, CSIC, 14004 Cordoba, SpainDifferent fungal species belonging to the <i>Colletotrichum</i> genus cause anthracnose disease in a range of major crops, resulting in huge economic losses worldwide. Typical symptoms include dark, sunken lesions on leaves, stems, or fruits. <i>Colletotrichum</i> spp. have synthesized, in vitro, a number of biologically active and structurally unusual metabolites that are involved in their host’s infection process. In this study, we applied a one strain many compounds (OSMAC) approach, integrated with targeted and non-targeted metabolomics profiling, to shed light on the secondary phytotoxic metabolite panels produced by pathogenic isolates of <i>Colletotrichum truncatum</i> and <i>Colletotrichum trifolii</i>. The phytotoxicity of the fungal crude extracts was also assessed on their primary hosts and related legumes, and the results correlated with the metabolite profile that arose from the different cultural conditions. To the best of our knowledge, this is the first time that the OSMAC strategy integrated with metabolomics approaches has been applied to <i>Colletotrichum</i> species involved in legume diseases.https://www.mdpi.com/2309-608X/9/6/610<i>Colletotrichum</i> spp.fungal metabolitesanthracnoselegumesphytotoxinsmetabolomics |
| spellingShingle | Pierluigi Reveglia Francisco J. Agudo-Jurado Eleonora Barilli Marco Masi Antonio Evidente Diego Rubiales Uncovering Phytotoxic Compounds Produced by <i>Colletotrichum</i> spp. Involved in Legume Diseases Using an OSMAC–Metabolomics Approach Journal of Fungi <i>Colletotrichum</i> spp. fungal metabolites anthracnose legumes phytotoxins metabolomics |
| title | Uncovering Phytotoxic Compounds Produced by <i>Colletotrichum</i> spp. Involved in Legume Diseases Using an OSMAC–Metabolomics Approach |
| title_full | Uncovering Phytotoxic Compounds Produced by <i>Colletotrichum</i> spp. Involved in Legume Diseases Using an OSMAC–Metabolomics Approach |
| title_fullStr | Uncovering Phytotoxic Compounds Produced by <i>Colletotrichum</i> spp. Involved in Legume Diseases Using an OSMAC–Metabolomics Approach |
| title_full_unstemmed | Uncovering Phytotoxic Compounds Produced by <i>Colletotrichum</i> spp. Involved in Legume Diseases Using an OSMAC–Metabolomics Approach |
| title_short | Uncovering Phytotoxic Compounds Produced by <i>Colletotrichum</i> spp. Involved in Legume Diseases Using an OSMAC–Metabolomics Approach |
| title_sort | uncovering phytotoxic compounds produced by i colletotrichum i spp involved in legume diseases using an osmac metabolomics approach |
| topic | <i>Colletotrichum</i> spp. fungal metabolites anthracnose legumes phytotoxins metabolomics |
| url | https://www.mdpi.com/2309-608X/9/6/610 |
| work_keys_str_mv | AT pierluigireveglia uncoveringphytotoxiccompoundsproducedbyicolletotrichumisppinvolvedinlegumediseasesusinganosmacmetabolomicsapproach AT franciscojagudojurado uncoveringphytotoxiccompoundsproducedbyicolletotrichumisppinvolvedinlegumediseasesusinganosmacmetabolomicsapproach AT eleonorabarilli uncoveringphytotoxiccompoundsproducedbyicolletotrichumisppinvolvedinlegumediseasesusinganosmacmetabolomicsapproach AT marcomasi uncoveringphytotoxiccompoundsproducedbyicolletotrichumisppinvolvedinlegumediseasesusinganosmacmetabolomicsapproach AT antonioevidente uncoveringphytotoxiccompoundsproducedbyicolletotrichumisppinvolvedinlegumediseasesusinganosmacmetabolomicsapproach AT diegorubiales uncoveringphytotoxiccompoundsproducedbyicolletotrichumisppinvolvedinlegumediseasesusinganosmacmetabolomicsapproach |